Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice

Detalhes bibliográficos
Autor(a) principal: Gonzaga, Beatriz Matheus Souza
Data de Publicação: 2022
Outros Autores: Horita, Samuel Iwao Maia, Beghini, Daniela Gois, Gomes, Fabiana, Nisimura, Líndice Mitie, Santos, Isabele Barbieri dos, Estato, Vanessa, Araújo‑Jorge, Tania Cremonini de, Garzoni, Luciana Ribeiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56468
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações Em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.
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spelling Gonzaga, Beatriz Matheus SouzaHorita, Samuel Iwao MaiaBeghini, Daniela GoisGomes, FabianaNisimura, Líndice MitieSantos, Isabele Barbieri dosEstato, VanessaAraújo‑Jorge, Tania Cremonini deGarzoni, Luciana Ribeiro2023-01-15T13:14:09Z2023-01-15T13:14:09Z2022GONZAGA, Beatriz Matheus Souza et al. Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice. Scientific Reports, v. 12, 21048, p. 1 - 11, 2022.2045-2322https://www.arca.fiocruz.br/handle/icict/5646810.1038/s41598-022-25056-xengNature PortfolioEfeito do benznidazolMicrocirculação cerebralDurante a Infecção por Trypanosoma cruziCamundongosEffect of benznidazoleCerebral microcirculationInfection in miceDuring Acute Trypanosoma cruziEffect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações Em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações Em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações Em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa Em Malária. Rio de Janeiro, RJ, Brasil.Instituto Carlos Chagas. Laboratório de Pesquisa Em Apicomplexa. Paraná, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Biotério do Pavilhão Carlos Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações Em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Central nervous system alterations was described in Chagas disease in both human and experimental models, leading to meningoencephalitis, stroke and cognitive impairment. Recently, our group demonstrated that acute infection by Trypanossoma cruzi leads to cerebral microvasculophaty in mice with endothelial dysfunction, capillary rarefaction, increased rolling and leukocyte adhesion. Only benznidazole and nifurtimox are available for clinical treatment, they have an efficiency of 80% in the acute phase and less than 20% in chronic phase. However, the effect of these drugs on brain microcirculation has not yet been evaluated. We hypothesized that early treatment with benznidazole could protect brain microcirculation during acute experimental Chagas disease. Swiss Webster mice were inoculated with 104 trypomastigotes forms of T. cruzi, and after 24 h they were treated with 50 or 100 mg/kg/day of benznidazole for 14 consecutive days. In untreated infected mice, we observed cerebral microvascular rarefaction, increase in leukocyte rolling and adhesion, reduced cerebral blood flow, and increased CD3+ and F4-80+ cells in brain tissue. Early treatment with benznidazole at 100 mg/kg/day and 50 mg/kg/day prevented the occurrence of the alterations mentioned. Here, we show that BZ is able to protect the microcirculation and reduced brain inflammation in acute experimental Chagas disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56468/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFabianaGomes_VanessaEstato_etal_IOC_2022.pdfFabianaGomes_VanessaEstato_etal_IOC_2022.pdfapplication/pdf2144078https://www.arca.fiocruz.br/bitstream/icict/56468/2/FabianaGomes_VanessaEstato_etal_IOC_2022.pdf2d8bd8624aee57054463b704e63826a3MD52icict/564682023-09-14 15:37:31.442oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T18:37:31Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice
title Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice
spellingShingle Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice
Gonzaga, Beatriz Matheus Souza
Efeito do benznidazol
Microcirculação cerebral
Durante a Infecção por Trypanosoma cruzi
Camundongos
Effect of benznidazole
Cerebral microcirculation
Infection in mice
During Acute Trypanosoma cruzi
title_short Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice
title_full Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice
title_fullStr Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice
title_full_unstemmed Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice
title_sort Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice
author Gonzaga, Beatriz Matheus Souza
author_facet Gonzaga, Beatriz Matheus Souza
Horita, Samuel Iwao Maia
Beghini, Daniela Gois
Gomes, Fabiana
Nisimura, Líndice Mitie
Santos, Isabele Barbieri dos
Estato, Vanessa
Araújo‑Jorge, Tania Cremonini de
Garzoni, Luciana Ribeiro
author_role author
author2 Horita, Samuel Iwao Maia
Beghini, Daniela Gois
Gomes, Fabiana
Nisimura, Líndice Mitie
Santos, Isabele Barbieri dos
Estato, Vanessa
Araújo‑Jorge, Tania Cremonini de
Garzoni, Luciana Ribeiro
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gonzaga, Beatriz Matheus Souza
Horita, Samuel Iwao Maia
Beghini, Daniela Gois
Gomes, Fabiana
Nisimura, Líndice Mitie
Santos, Isabele Barbieri dos
Estato, Vanessa
Araújo‑Jorge, Tania Cremonini de
Garzoni, Luciana Ribeiro
dc.subject.other.en_US.fl_str_mv Efeito do benznidazol
Microcirculação cerebral
Durante a Infecção por Trypanosoma cruzi
Camundongos
topic Efeito do benznidazol
Microcirculação cerebral
Durante a Infecção por Trypanosoma cruzi
Camundongos
Effect of benznidazole
Cerebral microcirculation
Infection in mice
During Acute Trypanosoma cruzi
dc.subject.en.en_US.fl_str_mv Effect of benznidazole
Cerebral microcirculation
Infection in mice
During Acute Trypanosoma cruzi
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações Em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-01-15T13:14:09Z
dc.date.available.fl_str_mv 2023-01-15T13:14:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GONZAGA, Beatriz Matheus Souza et al. Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice. Scientific Reports, v. 12, 21048, p. 1 - 11, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56468
dc.identifier.issn.en_US.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-022-25056-x
identifier_str_mv GONZAGA, Beatriz Matheus Souza et al. Effect of benznidazole on cerebral microcirculation during acute Trypanosoma cruzi infection in mice. Scientific Reports, v. 12, 21048, p. 1 - 11, 2022.
2045-2322
10.1038/s41598-022-25056-x
url https://www.arca.fiocruz.br/handle/icict/56468
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Portfolio
publisher.none.fl_str_mv Nature Portfolio
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/56468/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/56468/2/FabianaGomes_VanessaEstato_etal_IOC_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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