Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Gustavo
Data de Publicação: 2021
Outros Autores: Campos, Monique Paiva, Gonçalves, Alessandra Silva, Medeiros, Lia Carolina Soares, Figueiredo, Fabiano Borges
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50787
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
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spelling Gonçalves, GustavoCampos, Monique PaivaGonçalves, Alessandra SilvaMedeiros, Lia Carolina SoaresFigueiredo, Fabiano Borges2022-01-17T16:32:31Z2022-01-17T16:32:31Z2021GONÇALVES, Gustavo et al. Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol. Parasites & Vectors, v. 14, n. 599, p. 1–8, 2021.1756-3305https://www.arca.fiocruz.br/handle/icict/5078710.1186/s13071-021-05100-xporParasites & VectorsMiltefosinaAnfotericina BAmastigoteClinical isolateIn Vitro TechniquesPromastigoteDrug ResistanceResistencia a MedicamentosTechniques in vitroRésistance aux substancesLeishmaniaTécnicas In VitroResistência a MedicamentosIncreased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinolinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Private Practice, Campo Grande, Brazil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Leishmania infantum is the most important etiological agent of visceral leishmaniasis in the Americas and Mediterranean region, and the dog is the main host. Miltefosine was authorized to treat canine leishmaniasis (CanL) in Brazil in 2017, but there is a persistent fear of the emergence of parasites resistant not only to this drug but, through cross-resistance mechanisms, also to meglumine antimoniate and amphotericin B. Additionally, the literature shows that acquisition of resistance is followed by increased parasite ftness, with higher rates of proliferation, infectivity and metacyclogenesis, which are drivers of parasite virulence. In this context, the aim of this study was to analyze the impact of treating a dog with miltefosine and allopurinol on the generation of parasites resistant to miltefosine, amphotericin B and meglumine antimoniate. In vitro susceptibility tests were conducted against miltefosine, amphotericin B and meglumine antimoniate with T0 (parasites isolated from a dog before treatment with miltefosine plus allopurinol), T1 (after 1 course of treatment) and T2 (after 2 courses of treatment) isolates. The rates of cell proliferation, infectivity and metacyclogenesis of the isolates were also evaluated. The results indicate a gradual increase in parasite resistance to miltefosine and amphotericin B with increasing the number of treatment courses. An increasing trend in the metacyclogenesis rate of the parasites was also observed as drug resistance increased. The data indicates an increased L. infantum resistance to miltefosine and amphotericin B after the treatment of a dog with miltefosine plus allopurinol. Further studies with a larger number of L. infantum strains isolated from dogs with varied immune response profles and undergoing diferent treatment regimes, are advocated.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/50787/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL13071_2021_Article_5100OK.pdf13071_2021_Article_5100OK.pdfapplication/pdf1162936https://www.arca.fiocruz.br/bitstream/icict/50787/2/13071_2021_Article_5100OK.pdfd5833a2005b1e8a1f24e50c8aee93361MD52icict/507872022-01-17 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dc.title.pt_BR.fl_str_mv Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol
title Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol
spellingShingle Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol
Gonçalves, Gustavo
Miltefosina
Anfotericina B
Amastigote
Clinical isolate
In Vitro Techniques
Promastigote
Drug Resistance
Resistencia a Medicamentos
Techniques in vitro
Résistance aux substances
Leishmania
Técnicas In Vitro
Resistência a Medicamentos
title_short Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol
title_full Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol
title_fullStr Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol
title_full_unstemmed Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol
title_sort Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol
author Gonçalves, Gustavo
author_facet Gonçalves, Gustavo
Campos, Monique Paiva
Gonçalves, Alessandra Silva
Medeiros, Lia Carolina Soares
Figueiredo, Fabiano Borges
author_role author
author2 Campos, Monique Paiva
Gonçalves, Alessandra Silva
Medeiros, Lia Carolina Soares
Figueiredo, Fabiano Borges
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gonçalves, Gustavo
Campos, Monique Paiva
Gonçalves, Alessandra Silva
Medeiros, Lia Carolina Soares
Figueiredo, Fabiano Borges
dc.subject.other.pt_BR.fl_str_mv Miltefosina
Anfotericina B
topic Miltefosina
Anfotericina B
Amastigote
Clinical isolate
In Vitro Techniques
Promastigote
Drug Resistance
Resistencia a Medicamentos
Techniques in vitro
Résistance aux substances
Leishmania
Técnicas In Vitro
Resistência a Medicamentos
dc.subject.en.pt_BR.fl_str_mv Amastigote
Clinical isolate
In Vitro Techniques
Promastigote
Drug Resistance
dc.subject.es.pt_BR.fl_str_mv Resistencia a Medicamentos
dc.subject.fr.pt_BR.fl_str_mv Techniques in vitro
Résistance aux substances
dc.subject.decs.pt_BR.fl_str_mv Leishmania
Técnicas In Vitro
Resistência a Medicamentos
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-01-17T16:32:31Z
dc.date.available.fl_str_mv 2022-01-17T16:32:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GONÇALVES, Gustavo et al. Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol. Parasites & Vectors, v. 14, n. 599, p. 1–8, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50787
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-021-05100-x
identifier_str_mv GONÇALVES, Gustavo et al. Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol. Parasites & Vectors, v. 14, n. 599, p. 1–8, 2021.
1756-3305
10.1186/s13071-021-05100-x
url https://www.arca.fiocruz.br/handle/icict/50787
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Parasites & Vectors
publisher.none.fl_str_mv Parasites & Vectors
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50787/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50787/2/13071_2021_Article_5100OK.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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