Analysis of Schistosoma mansoni population structure using total fecal egg sampling

Detalhes bibliográficos
Autor(a) principal: Blank, Walter A.
Data de Publicação: 2009
Outros Autores: Reis, Eliana Almeida Gomes, Thiong'o, Fred W., Braghiroli, João F., Santos, Jarbas M., Melo, Paulo Roberto Santana de, Guimarães, Isabel C. S., Silva, Luciano Kalabric, Carmo, Theomira Mauadie de Azevedo, Reis, Mitermayer Galvão dos, Blanton, Ronald Edward
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7521
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
id CRUZ_31b32b825ca6ac71539ab0120d737ed1
oai_identifier_str oai:www.arca.fiocruz.br:icict/7521
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Blank, Walter A.Reis, Eliana Almeida GomesThiong'o, Fred W.Braghiroli, João F.Santos, Jarbas M.Melo, Paulo Roberto Santana deGuimarães, Isabel C. S.Silva, Luciano KalabricCarmo, Theomira Mauadie de AzevedoReis, Mitermayer Galvão dosBlanton, Ronald Edward2014-04-14T18:58:05Z2014-04-14T18:58:05Z2009BLANK, W. A. et al. Analysis of Schistosoma mansoni population structure using total fecal egg sampling. Journal of Parasitolofy, v. 95, n. 4, p. 881-889, 2009.1937-2345https://www.arca.fiocruz.br/handle/icict/752110.1645/GE-1895.1engNational Institute of HealthAnalysis of Schistosoma mansoni population structure using total fecal egg samplinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.University of Nairobi. Nairobi, Kenya / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Center for Global Health and Diseases. Case Western Reserve University. Cleveland, Ohio.Many parasite populations are difficult to sample because they are not uniformly distributed between several host species and are often not easily collected from the living host, thereby limiting sample size and possibly distorting the representation of the population. For the parasite Schistosoma mansoni, we investigated the use of eggs, in aggregate, from the stools of infected individuals as a simple and representative sample. Previously, we demonstrated that microsatellite allele frequencies can be accurately estimated from pooled DNA of cloned S. mansoni adults. Here, we show that genotyping of parasite populations from reproductively isolated laboratory strains can be used to identify these specific populations based on characteristic patterns of allele frequencies, as observed by polyacrylamide gel electrophoresis and automated sequencer analysis of fluorescently labeled PCR products. Microsatellites used to genotype aggregates of eggs collected from stools of infected individuals produced results consistent with the geographic distribution of the samples. Preferential amplification of smaller alleles, and stutter PCR products, had negligible effect on measurement of genetic differentiation. Direct analysis of total stool eggs can be an important approach to questions of population genetics for this parasite by increasing the sample size to thousands per infected individual and by reducing bias.Schistosoma MansoniFecal egg samplingDNA de Helmintos/químicaFezes/parasitologiaRepetições de Microssatélites/genéticaSchistosoma mansoni/isolamento & purificaçãoEsquistossomose mansoni/parasitologiaEletroforese em Gel de PoliacrilamidaAnimaisBrasilFemininoFrequência do GeneGenótipoHumanosQuêniaMasculinoÓvuloReação em Cadeia da PolimeraseSchistosoma mansoni/classificaçãoSchistosoma mansoni/genéticaAnálise de Sequênciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBlank W A Direct analysis of .....pdfBlank W A Direct analysis of .....pdfapplication/pdf616884https://www.arca.fiocruz.br/bitstream/icict/7521/1/Blank%20W%20A%20Direct%20analysis%20of%20.....pdf11d9b6dea1cdf018a1bac91058c6ecdfMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7521/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBlank W A Direct analysis of .....pdf.txtBlank W A Direct analysis of .....pdf.txtExtracted texttext/plain38538https://www.arca.fiocruz.br/bitstream/icict/7521/5/Blank%20W%20A%20Direct%20analysis%20of%20.....pdf.txtae8857339164d03216b6ee8ced883e08MD55THUMBNAILBlank W A Direct analysis of .....pdf.jpgBlank W A Direct analysis of .....pdf.jpgGenerated Thumbnailimage/jpeg2399https://www.arca.fiocruz.br/bitstream/icict/7521/4/Blank%20W%20A%20Direct%20analysis%20of%20.....pdf.jpg6cb726215775f41b5e7b58cb40784d71MD54icict/75212023-03-15 14:33:01.941oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Analysis of Schistosoma mansoni population structure using total fecal egg sampling
title Analysis of Schistosoma mansoni population structure using total fecal egg sampling
spellingShingle Analysis of Schistosoma mansoni population structure using total fecal egg sampling
Blank, Walter A.
Schistosoma Mansoni
Fecal egg sampling
DNA de Helmintos/química
Fezes/parasitologia
Repetições de Microssatélites/genética
Schistosoma mansoni/isolamento & purificação
Esquistossomose mansoni/parasitologia
Eletroforese em Gel de Poliacrilamida
Animais
Brasil
Feminino
Frequência do Gene
Genótipo
Humanos
Quênia
Masculino
Óvulo
Reação em Cadeia da Polimerase
Schistosoma mansoni/classificação
Schistosoma mansoni/genética
Análise de Sequência
title_short Analysis of Schistosoma mansoni population structure using total fecal egg sampling
title_full Analysis of Schistosoma mansoni population structure using total fecal egg sampling
title_fullStr Analysis of Schistosoma mansoni population structure using total fecal egg sampling
title_full_unstemmed Analysis of Schistosoma mansoni population structure using total fecal egg sampling
title_sort Analysis of Schistosoma mansoni population structure using total fecal egg sampling
author Blank, Walter A.
author_facet Blank, Walter A.
Reis, Eliana Almeida Gomes
Thiong'o, Fred W.
Braghiroli, João F.
Santos, Jarbas M.
Melo, Paulo Roberto Santana de
Guimarães, Isabel C. S.
Silva, Luciano Kalabric
Carmo, Theomira Mauadie de Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
author_role author
author2 Reis, Eliana Almeida Gomes
Thiong'o, Fred W.
Braghiroli, João F.
Santos, Jarbas M.
Melo, Paulo Roberto Santana de
Guimarães, Isabel C. S.
Silva, Luciano Kalabric
Carmo, Theomira Mauadie de Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Blank, Walter A.
Reis, Eliana Almeida Gomes
Thiong'o, Fred W.
Braghiroli, João F.
Santos, Jarbas M.
Melo, Paulo Roberto Santana de
Guimarães, Isabel C. S.
Silva, Luciano Kalabric
Carmo, Theomira Mauadie de Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
dc.subject.en.pt_BR.fl_str_mv Schistosoma Mansoni
Fecal egg sampling
topic Schistosoma Mansoni
Fecal egg sampling
DNA de Helmintos/química
Fezes/parasitologia
Repetições de Microssatélites/genética
Schistosoma mansoni/isolamento & purificação
Esquistossomose mansoni/parasitologia
Eletroforese em Gel de Poliacrilamida
Animais
Brasil
Feminino
Frequência do Gene
Genótipo
Humanos
Quênia
Masculino
Óvulo
Reação em Cadeia da Polimerase
Schistosoma mansoni/classificação
Schistosoma mansoni/genética
Análise de Sequência
dc.subject.decs.pt_BR.fl_str_mv DNA de Helmintos/química
Fezes/parasitologia
Repetições de Microssatélites/genética
Schistosoma mansoni/isolamento & purificação
Esquistossomose mansoni/parasitologia
Eletroforese em Gel de Poliacrilamida
Animais
Brasil
Feminino
Frequência do Gene
Genótipo
Humanos
Quênia
Masculino
Óvulo
Reação em Cadeia da Polimerase
Schistosoma mansoni/classificação
Schistosoma mansoni/genética
Análise de Sequência
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2014-04-14T18:58:05Z
dc.date.available.fl_str_mv 2014-04-14T18:58:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BLANK, W. A. et al. Analysis of Schistosoma mansoni population structure using total fecal egg sampling. Journal of Parasitolofy, v. 95, n. 4, p. 881-889, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7521
dc.identifier.issn.none.fl_str_mv 1937-2345
dc.identifier.doi.pt_BR.fl_str_mv 10.1645/GE-1895.1
identifier_str_mv BLANK, W. A. et al. Analysis of Schistosoma mansoni population structure using total fecal egg sampling. Journal of Parasitolofy, v. 95, n. 4, p. 881-889, 2009.
1937-2345
10.1645/GE-1895.1
url https://www.arca.fiocruz.br/handle/icict/7521
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Institute of Health
publisher.none.fl_str_mv National Institute of Health
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/7521/1/Blank%20W%20A%20Direct%20analysis%20of%20.....pdf
https://www.arca.fiocruz.br/bitstream/icict/7521/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/7521/5/Blank%20W%20A%20Direct%20analysis%20of%20.....pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/7521/4/Blank%20W%20A%20Direct%20analysis%20of%20.....pdf.jpg
bitstream.checksum.fl_str_mv 11d9b6dea1cdf018a1bac91058c6ecdf
7d48279ffeed55da8dfe2f8e81f3b81f
ae8857339164d03216b6ee8ced883e08
6cb726215775f41b5e7b58cb40784d71
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324942701330432

Registros relacionados