Analysis of Schistosoma mansoni population structure using total fecal egg sampling
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7521 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil. |
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Blank, Walter A.Reis, Eliana Almeida GomesThiong'o, Fred W.Braghiroli, João F.Santos, Jarbas M.Melo, Paulo Roberto Santana deGuimarães, Isabel C. S.Silva, Luciano KalabricCarmo, Theomira Mauadie de AzevedoReis, Mitermayer Galvão dosBlanton, Ronald Edward2014-04-14T18:58:05Z2014-04-14T18:58:05Z2009BLANK, W. A. et al. Analysis of Schistosoma mansoni population structure using total fecal egg sampling. Journal of Parasitolofy, v. 95, n. 4, p. 881-889, 2009.1937-2345https://www.arca.fiocruz.br/handle/icict/752110.1645/GE-1895.1engNational Institute of HealthAnalysis of Schistosoma mansoni population structure using total fecal egg samplinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.University of Nairobi. Nairobi, Kenya / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Center for Global Health and Diseases. Case Western Reserve University. Cleveland, Ohio.Many parasite populations are difficult to sample because they are not uniformly distributed between several host species and are often not easily collected from the living host, thereby limiting sample size and possibly distorting the representation of the population. For the parasite Schistosoma mansoni, we investigated the use of eggs, in aggregate, from the stools of infected individuals as a simple and representative sample. Previously, we demonstrated that microsatellite allele frequencies can be accurately estimated from pooled DNA of cloned S. mansoni adults. Here, we show that genotyping of parasite populations from reproductively isolated laboratory strains can be used to identify these specific populations based on characteristic patterns of allele frequencies, as observed by polyacrylamide gel electrophoresis and automated sequencer analysis of fluorescently labeled PCR products. Microsatellites used to genotype aggregates of eggs collected from stools of infected individuals produced results consistent with the geographic distribution of the samples. Preferential amplification of smaller alleles, and stutter PCR products, had negligible effect on measurement of genetic differentiation. Direct analysis of total stool eggs can be an important approach to questions of population genetics for this parasite by increasing the sample size to thousands per infected individual and by reducing bias.Schistosoma MansoniFecal egg samplingDNA de Helmintos/químicaFezes/parasitologiaRepetições de Microssatélites/genéticaSchistosoma mansoni/isolamento & purificaçãoEsquistossomose mansoni/parasitologiaEletroforese em Gel de PoliacrilamidaAnimaisBrasilFemininoFrequência do GeneGenótipoHumanosQuêniaMasculinoÓvuloReação em Cadeia da PolimeraseSchistosoma mansoni/classificaçãoSchistosoma mansoni/genéticaAnálise de Sequênciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBlank W A Direct analysis of .....pdfBlank W A Direct analysis of .....pdfapplication/pdf616884https://www.arca.fiocruz.br/bitstream/icict/7521/1/Blank%20W%20A%20Direct%20analysis%20of%20.....pdf11d9b6dea1cdf018a1bac91058c6ecdfMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7521/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBlank W A Direct analysis of .....pdf.txtBlank W A Direct analysis of .....pdf.txtExtracted texttext/plain38538https://www.arca.fiocruz.br/bitstream/icict/7521/5/Blank%20W%20A%20Direct%20analysis%20of%20.....pdf.txtae8857339164d03216b6ee8ced883e08MD55THUMBNAILBlank W A Direct analysis of .....pdf.jpgBlank W A Direct analysis of .....pdf.jpgGenerated Thumbnailimage/jpeg2399https://www.arca.fiocruz.br/bitstream/icict/7521/4/Blank%20W%20A%20Direct%20analysis%20of%20.....pdf.jpg6cb726215775f41b5e7b58cb40784d71MD54icict/75212023-03-15 14:33:01.941oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Analysis of Schistosoma mansoni population structure using total fecal egg sampling |
title |
Analysis of Schistosoma mansoni population structure using total fecal egg sampling |
spellingShingle |
Analysis of Schistosoma mansoni population structure using total fecal egg sampling Blank, Walter A. Schistosoma Mansoni Fecal egg sampling DNA de Helmintos/química Fezes/parasitologia Repetições de Microssatélites/genética Schistosoma mansoni/isolamento & purificação Esquistossomose mansoni/parasitologia Eletroforese em Gel de Poliacrilamida Animais Brasil Feminino Frequência do Gene Genótipo Humanos Quênia Masculino Óvulo Reação em Cadeia da Polimerase Schistosoma mansoni/classificação Schistosoma mansoni/genética Análise de Sequência |
title_short |
Analysis of Schistosoma mansoni population structure using total fecal egg sampling |
title_full |
Analysis of Schistosoma mansoni population structure using total fecal egg sampling |
title_fullStr |
Analysis of Schistosoma mansoni population structure using total fecal egg sampling |
title_full_unstemmed |
Analysis of Schistosoma mansoni population structure using total fecal egg sampling |
title_sort |
Analysis of Schistosoma mansoni population structure using total fecal egg sampling |
author |
Blank, Walter A. |
author_facet |
Blank, Walter A. Reis, Eliana Almeida Gomes Thiong'o, Fred W. Braghiroli, João F. Santos, Jarbas M. Melo, Paulo Roberto Santana de Guimarães, Isabel C. S. Silva, Luciano Kalabric Carmo, Theomira Mauadie de Azevedo Reis, Mitermayer Galvão dos Blanton, Ronald Edward |
author_role |
author |
author2 |
Reis, Eliana Almeida Gomes Thiong'o, Fred W. Braghiroli, João F. Santos, Jarbas M. Melo, Paulo Roberto Santana de Guimarães, Isabel C. S. Silva, Luciano Kalabric Carmo, Theomira Mauadie de Azevedo Reis, Mitermayer Galvão dos Blanton, Ronald Edward |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Blank, Walter A. Reis, Eliana Almeida Gomes Thiong'o, Fred W. Braghiroli, João F. Santos, Jarbas M. Melo, Paulo Roberto Santana de Guimarães, Isabel C. S. Silva, Luciano Kalabric Carmo, Theomira Mauadie de Azevedo Reis, Mitermayer Galvão dos Blanton, Ronald Edward |
dc.subject.en.pt_BR.fl_str_mv |
Schistosoma Mansoni Fecal egg sampling |
topic |
Schistosoma Mansoni Fecal egg sampling DNA de Helmintos/química Fezes/parasitologia Repetições de Microssatélites/genética Schistosoma mansoni/isolamento & purificação Esquistossomose mansoni/parasitologia Eletroforese em Gel de Poliacrilamida Animais Brasil Feminino Frequência do Gene Genótipo Humanos Quênia Masculino Óvulo Reação em Cadeia da Polimerase Schistosoma mansoni/classificação Schistosoma mansoni/genética Análise de Sequência |
dc.subject.decs.pt_BR.fl_str_mv |
DNA de Helmintos/química Fezes/parasitologia Repetições de Microssatélites/genética Schistosoma mansoni/isolamento & purificação Esquistossomose mansoni/parasitologia Eletroforese em Gel de Poliacrilamida Animais Brasil Feminino Frequência do Gene Genótipo Humanos Quênia Masculino Óvulo Reação em Cadeia da Polimerase Schistosoma mansoni/classificação Schistosoma mansoni/genética Análise de Sequência |
description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2014-04-14T18:58:05Z |
dc.date.available.fl_str_mv |
2014-04-14T18:58:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BLANK, W. A. et al. Analysis of Schistosoma mansoni population structure using total fecal egg sampling. Journal of Parasitolofy, v. 95, n. 4, p. 881-889, 2009. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/7521 |
dc.identifier.issn.none.fl_str_mv |
1937-2345 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1645/GE-1895.1 |
identifier_str_mv |
BLANK, W. A. et al. Analysis of Schistosoma mansoni population structure using total fecal egg sampling. Journal of Parasitolofy, v. 95, n. 4, p. 881-889, 2009. 1937-2345 10.1645/GE-1895.1 |
url |
https://www.arca.fiocruz.br/handle/icict/7521 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
National Institute of Health |
publisher.none.fl_str_mv |
National Institute of Health |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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