Serological and molecular evidence of hepadnavirus infection in swine
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12110 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. |
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Vieira, Yasmine R.Silva, Marcelle F. M.Santos, Débora R. L.Vieira, Antônio A.Zanella, Janice R. CiacciBarquero, GonzaloLago, Bárbara V. doGomes, Selma A.Pinto, Marcelo A.Paula, Vanessa S. de2015-11-03T15:49:16Z2015-11-03T15:49:16Z2015VIEIRA, Yasmine R. et al. Serological and molecular evidence of hepadnavirus infection in swine. Annals of Agricultural and Environmental Medicine, v. 22, n.1, p.11–16, 2015.1232-1966https://www.arca.fiocruz.br/handle/icict/1211010.5604/12321966.11413611898-2263engNational Center for Biotechnology InformationSerological and molecular evidence of hepadnavirus infection in swineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Departamento de Microbiologia e Imunobiologia Veterinária. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Ciência Aninmal. Departamento de Produção Animal. Seropédica, RJ, Brasil.EMBRAPA Suínos e Aves. Centro de Pesquisa Nacional. Santa Catarina, Brasil.Instituto Tropical de Sustentabilidade. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Abstract Introduction and objective. Recently, investigations in a swine herd identified evidence of the existence of a novel member of the Hepadnavirus family endemic in swine. The aim of this study was to investigate the serological and molecular markers of Hepadnavirus circulation in Brazilian domestic swine and wild boar herds, and to evaluate the identity with HBV and other Hepadnaviruses reported previously. Materials and methods. For the study, 376 swine were screened for hepatitis B virus serological markers. Analyses were performed in serum samples using commercial enzyme-linked immunosorbent assay (ELISA) kits (DiaSorin®) for anti-HBc, HBsAg and anti-HBs. Reactive and undetermined swine serum samples were selected to perform DNA viral extraction (QIAamp DNA Mini Kit, Qiagen®), partial genome amplification and genome sequencing. Results. From 376 swine samples analysed, 28 (7.45%) were reactive to anti-HBc, 3 (0.80%) to HBsAg and 6 (1.6%) to anti-HBs. Besides, more 17 (4.52%) swine samples analyzed were classified in the grey zone of the EIA test to anti-HBc and 2 (0.53%) to HBsAg. From 49 samples molecularly analyzed after serological trial, 4 samples showed a positive result for the qualitative PCR for Hepadnavirus. Phylogenetic reconstruction using partial genome sequencing (360 bp) of 3 samples showed similarity with HBV with 90.8–96.3% of identity. Conclusions. Serological and molecular data showed evidence of the circulation of a virus similar to hepatitis B virus in swine.DiagnosticHepadnavirusSwineSuínosDiagnósticoVírus da Hepatite Binfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/12110/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALyaseminie_vieira_etal_IOC_2015.pdfyaseminie_vieira_etal_IOC_2015.pdfapplication/pdf658834https://www.arca.fiocruz.br/bitstream/icict/12110/2/yaseminie_vieira_etal_IOC_2015.pdfbb6a8ae842ad68daf8084f57bc745b08MD52TEXTyaseminie_vieira_etal_IOC_2015.pdf.txtyaseminie_vieira_etal_IOC_2015.pdf.txtExtracted texttext/plain35671https://www.arca.fiocruz.br/bitstream/icict/12110/3/yaseminie_vieira_etal_IOC_2015.pdf.txt37d0faa68ab9ecfc1ff96e55163e83eaMD53icict/121102023-09-05 10:36:30.023oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:36:30Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Serological and molecular evidence of hepadnavirus infection in swine |
| title |
Serological and molecular evidence of hepadnavirus infection in swine |
| spellingShingle |
Serological and molecular evidence of hepadnavirus infection in swine Vieira, Yasmine R. Diagnostic Hepadnavirus Swine Suínos Diagnóstico Vírus da Hepatite B |
| title_short |
Serological and molecular evidence of hepadnavirus infection in swine |
| title_full |
Serological and molecular evidence of hepadnavirus infection in swine |
| title_fullStr |
Serological and molecular evidence of hepadnavirus infection in swine |
| title_full_unstemmed |
Serological and molecular evidence of hepadnavirus infection in swine |
| title_sort |
Serological and molecular evidence of hepadnavirus infection in swine |
| author |
Vieira, Yasmine R. |
| author_facet |
Vieira, Yasmine R. Silva, Marcelle F. M. Santos, Débora R. L. Vieira, Antônio A. Zanella, Janice R. Ciacci Barquero, Gonzalo Lago, Bárbara V. do Gomes, Selma A. Pinto, Marcelo A. Paula, Vanessa S. de |
| author_role |
author |
| author2 |
Silva, Marcelle F. M. Santos, Débora R. L. Vieira, Antônio A. Zanella, Janice R. Ciacci Barquero, Gonzalo Lago, Bárbara V. do Gomes, Selma A. Pinto, Marcelo A. Paula, Vanessa S. de |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vieira, Yasmine R. Silva, Marcelle F. M. Santos, Débora R. L. Vieira, Antônio A. Zanella, Janice R. Ciacci Barquero, Gonzalo Lago, Bárbara V. do Gomes, Selma A. Pinto, Marcelo A. Paula, Vanessa S. de |
| dc.subject.en.pt_BR.fl_str_mv |
Diagnostic Hepadnavirus Swine |
| topic |
Diagnostic Hepadnavirus Swine Suínos Diagnóstico Vírus da Hepatite B |
| dc.subject.decs.pt_BR.fl_str_mv |
Suínos Diagnóstico Vírus da Hepatite B |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. |
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2015 |
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2015-11-03T15:49:16Z |
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2015-11-03T15:49:16Z |
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2015 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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VIEIRA, Yasmine R. et al. Serological and molecular evidence of hepadnavirus infection in swine. Annals of Agricultural and Environmental Medicine, v. 22, n.1, p.11–16, 2015. |
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https://www.arca.fiocruz.br/handle/icict/12110 |
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1232-1966 |
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10.5604/12321966.1141361 |
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1898-2263 |
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VIEIRA, Yasmine R. et al. Serological and molecular evidence of hepadnavirus infection in swine. Annals of Agricultural and Environmental Medicine, v. 22, n.1, p.11–16, 2015. 1232-1966 10.5604/12321966.1141361 1898-2263 |
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National Center for Biotechnology Information |
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National Center for Biotechnology Information |
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