De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing

Detalhes bibliográficos
Autor(a) principal: Santos, Leonardo Nascimento
Data de Publicação: 2016
Outros Autores: Silva, Eduardo Santos da, Santos, André S, Sá, Pablo Henrique Caracciolo Gomes de, Ramos, Rommel Thiago Juca, Silva, Artur Luiz da Costa da, Cooper, Philip J, Barreto, Maurício Lima, Loureiro, Sebastião, Pinheiro, Carina da Silva, Neves, Neuza Maria Alcântara, Pacheco, Luis Gustavo Carvalho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17959
Resumo: INCT/MCT/CNPq Programme – Contract no. 5737862008; MCTI/CNPq/FNDCT Ação Transversal N◦79/2013 (RENORBIO); FAPESB (PET0005/2010 and JCB017/2013); and CAPES (projectn◦077 2012).
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spelling Santos, Leonardo NascimentoSilva, Eduardo Santos daSantos, André SSá, Pablo Henrique Caracciolo Gomes deRamos, Rommel Thiago JucaSilva, Artur Luiz da Costa daCooper, Philip JBarreto, Maurício LimaLoureiro, SebastiãoPinheiro, Carina da SilvaNeves, Neuza Maria AlcântaraPacheco, Luis Gustavo Carvalho2017-03-07T14:08:55Z2017-03-07T14:08:55Z2016SANTOS, L. N. et al. De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing. Acta Tropica, v. 159, p. 132–141, 2016.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/1795910.1016/j.actatropica.2016.03.036INCT/MCT/CNPq Programme – Contract no. 5737862008; MCTI/CNPq/FNDCT Ação Transversal N◦79/2013 (RENORBIO); FAPESB (PET0005/2010 and JCB017/2013); and CAPES (projectn◦077 2012).Federal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilFederal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilFederal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilFederal University of Pará. Institute of Biological Sciences. Belém, PA, BrazilFederal University of Pará. Institute of Biological Sciences. Belém, PA, BrazilFederal University of Pará. Institute of Biological Sciences. Belém, PA, BrazilSt. George’s University of London. Institute of Infection and Immunity. London, UK / Pontificia Universidad Catolica del Ecuador. Centro de Investigacion en Enfermedades Infecciosas y Cronicas. Quito, EcuadorFederal University of Bahia. Institute of Public Health. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Institute of Public Health. Salvador, BA, BrazilFederal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilFederal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilFederal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilInfection with helminthic parasites, including the soil-transmitted helminth Trichuris trichiura (human whipworm), has been shown to modulate host immune responses and, consequently, to have an impact on the development and manifestation of chronic human inflammatory diseases. De novo derivation of helminth proteomes from sequencing of transcriptomes will provide valuable data to aid identification of parasite proteins that could be evaluated as potential immunotherapeutic molecules in near future. Herein, we characterized the transcriptome of the adult stage of the human whipworm T. trichiura, using next-generation sequencing technology and a de novo assembly strategy. Nearly 17.6 million high-quality clean reads were assembled into 6414 contiguous sequences, with an N50 of 1606bp. In total, 5673 protein-encoding sequences were confidentially identified in the T. trichiura adult worm transcriptome; of these, 1013 sequences represent potential newly discovered proteins for the species, most of which presenting orthologs already annotated in the related species T. suis. A number of transcripts representing probable novel non-coding transcripts for the species T. trichiura were also identified. Among the most abundant transcripts, we found sequences that code for proteins involved in lipid transport, such as vitellogenins, and several chitin-binding proteins. Through a cross-species expression analysis of gene orthologs shared by T. trichiura and the closely related parasites T. suis and T. muris it was possible to find twenty-six protein-encoding genes that are consistently highly expressed in the adult stages of the three helminth species. Additionally, twenty transcripts could be identified that code for proteins previously detected by mass spectrometry analysis of protein fractions of the whipworm somatic extract that present immunomodulatory activities. Five of these transcripts were amongst the most highly expressed protein-encoding sequences in the T. trichiura adult worm. Besides, orthologs of proteins demonstrated to have potent immunomodulatory properties in related parasitic helminths were also predicted from the T. trichiura de novo assembled transcriptome.engElsevierTrichuris trichiuraTranscriptomeSeqüenciamento da próxima geraçãoGenômica funcionalTrichuris trichiuraTranscriptomeNext-generation sequencingFunctional genomicsDe novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17959/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos LN De novo assembly....pdfSantos LN De novo 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dc.title.pt_BR.fl_str_mv De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing
title De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing
spellingShingle De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing
Santos, Leonardo Nascimento
Trichuris trichiura
Transcriptome
Seqüenciamento da próxima geração
Genômica funcional
Trichuris trichiura
Transcriptome
Next-generation sequencing
Functional genomics
title_short De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing
title_full De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing
title_fullStr De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing
title_full_unstemmed De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing
title_sort De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing
author Santos, Leonardo Nascimento
author_facet Santos, Leonardo Nascimento
Silva, Eduardo Santos da
Santos, André S
Sá, Pablo Henrique Caracciolo Gomes de
Ramos, Rommel Thiago Juca
Silva, Artur Luiz da Costa da
Cooper, Philip J
Barreto, Maurício Lima
Loureiro, Sebastião
Pinheiro, Carina da Silva
Neves, Neuza Maria Alcântara
Pacheco, Luis Gustavo Carvalho
author_role author
author2 Silva, Eduardo Santos da
Santos, André S
Sá, Pablo Henrique Caracciolo Gomes de
Ramos, Rommel Thiago Juca
Silva, Artur Luiz da Costa da
Cooper, Philip J
Barreto, Maurício Lima
Loureiro, Sebastião
Pinheiro, Carina da Silva
Neves, Neuza Maria Alcântara
Pacheco, Luis Gustavo Carvalho
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Leonardo Nascimento
Silva, Eduardo Santos da
Santos, André S
Sá, Pablo Henrique Caracciolo Gomes de
Ramos, Rommel Thiago Juca
Silva, Artur Luiz da Costa da
Cooper, Philip J
Barreto, Maurício Lima
Loureiro, Sebastião
Pinheiro, Carina da Silva
Neves, Neuza Maria Alcântara
Pacheco, Luis Gustavo Carvalho
dc.subject.other.pt_BR.fl_str_mv Trichuris trichiura
Transcriptome
Seqüenciamento da próxima geração
Genômica funcional
topic Trichuris trichiura
Transcriptome
Seqüenciamento da próxima geração
Genômica funcional
Trichuris trichiura
Transcriptome
Next-generation sequencing
Functional genomics
dc.subject.en.pt_BR.fl_str_mv Trichuris trichiura
Transcriptome
Next-generation sequencing
Functional genomics
description INCT/MCT/CNPq Programme – Contract no. 5737862008; MCTI/CNPq/FNDCT Ação Transversal N◦79/2013 (RENORBIO); FAPESB (PET0005/2010 and JCB017/2013); and CAPES (projectn◦077 2012).
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-07T14:08:55Z
dc.date.available.fl_str_mv 2017-03-07T14:08:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, L. N. et al. De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing. Acta Tropica, v. 159, p. 132–141, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17959
dc.identifier.issn.pt_BR.fl_str_mv 0001-706X
dc.identifier.doi.none.fl_str_mv 10.1016/j.actatropica.2016.03.036
identifier_str_mv SANTOS, L. N. et al. De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing. Acta Tropica, v. 159, p. 132–141, 2016.
0001-706X
10.1016/j.actatropica.2016.03.036
url https://www.arca.fiocruz.br/handle/icict/17959
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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