Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni worms

Detalhes bibliográficos
Autor(a) principal: Moraes, Vanessa Silva
Data de Publicação: 2013
Outros Autores: Couto, Flávia Fernanda Bubula, Vasconcelos, Marah Mileib, Araújo, Neusa, Coelho, Paulo Marcos Zech, Katz, Naftale, Grenfell, Rafaella Fortini Queiroz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59432
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Universidade Federal de São João Del Rey. Divinópolis, MG, Brasil.
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spelling Moraes, Vanessa SilvaCouto, Flávia Fernanda BubulaVasconcelos, Marah MileibAraújo, NeusaCoelho, Paulo Marcos ZechKatz, NaftaleGrenfell, Rafaella Fortini Queiroz2023-07-06T11:24:33Z2023-07-06T11:24:33Z2013MORAES, Vanessa Silva et al. Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni Worms. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 5, p. 600-604, 2013.0074-0276https://www.arca.fiocruz.br/handle/icict/5943210.1590/0074-0276108052013011engFiocruz/IOCAntischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni wormsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Universidade Federal de São João Del Rey. Divinópolis, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.Universidade de Itaúna. Escola de Farmácia. Itaúna, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Universidade de Itaúna. Escola de Farmácia. Itaúna, MG, Brasil.Current schistosomiasis control strategies are largely based on chemotherapeutic agents and a limited number of drugs are available today. Praziquantel (PZQ) is the only drug currently used in schistosomiasis control programs. Unfortunately, this drug shows poor efficacy in patients during the earliest infection phases. The effects of PZQ appear to operate on the voltage-operated Ca2+ channels, which are located on the external Schistosoma mansoni membrane. Because some Ca2+ channels have dihydropyridine drug class (a class that includes nifedipine) sensitivity, an in vitro analysis using a calcium channel antagonist (clinically used for cardiovascular hypertension) was performed to determine the antischistosomal effects of nifedipine on schistosomula and adult worm cultures. Nifedipine demonstrated antischistosomal activity against schistosomula and significantly reduced viability at all of the concentrations used alone or in combination with PZQ. In contrast, PZQ did not show significant efficacy when used alone. Adult worms were also affected by nifedipine after a 24 h incubation and exhibited impaired motility, several lesions on the tegument and intense contractility. These data support the idea of Ca2+ channels subunits as drug targets and favour alternative therapeutic schemes when drug resistance has been reported. In this paper, strong arguments encouraging drug research are presented, with a focus on exploring schistosomal Ca2+ channels.Schistosoma mansoniAntischistosomal drugsCalcium channels antagonistsL-type calcium channelsNifedipineinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL0074-0276-mioc-108-05-600.pdf0074-0276-mioc-108-05-600.pdfapplication/pdf410048https://www.arca.fiocruz.br/bitstream/icict/59432/2/0074-0276-mioc-108-05-600.pdf9891e4093facd52fdca12d0cff9a3eb9MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59432/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/594322023-07-06 22:07:47.939oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-07T01:07:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni worms
title Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni worms
spellingShingle Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni worms
Moraes, Vanessa Silva
Schistosoma mansoni
Antischistosomal drugs
Calcium channels antagonists
L-type calcium channels
Nifedipine
title_short Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni worms
title_full Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni worms
title_fullStr Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni worms
title_full_unstemmed Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni worms
title_sort Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni worms
author Moraes, Vanessa Silva
author_facet Moraes, Vanessa Silva
Couto, Flávia Fernanda Bubula
Vasconcelos, Marah Mileib
Araújo, Neusa
Coelho, Paulo Marcos Zech
Katz, Naftale
Grenfell, Rafaella Fortini Queiroz
author_role author
author2 Couto, Flávia Fernanda Bubula
Vasconcelos, Marah Mileib
Araújo, Neusa
Coelho, Paulo Marcos Zech
Katz, Naftale
Grenfell, Rafaella Fortini Queiroz
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moraes, Vanessa Silva
Couto, Flávia Fernanda Bubula
Vasconcelos, Marah Mileib
Araújo, Neusa
Coelho, Paulo Marcos Zech
Katz, Naftale
Grenfell, Rafaella Fortini Queiroz
dc.subject.en.en_US.fl_str_mv Schistosoma mansoni
Antischistosomal drugs
Calcium channels antagonists
L-type calcium channels
Nifedipine
topic Schistosoma mansoni
Antischistosomal drugs
Calcium channels antagonists
L-type calcium channels
Nifedipine
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Universidade Federal de São João Del Rey. Divinópolis, MG, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2023-07-06T11:24:33Z
dc.date.available.fl_str_mv 2023-07-06T11:24:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MORAES, Vanessa Silva et al. Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni Worms. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 5, p. 600-604, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59432
dc.identifier.issn.en_US.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-0276108052013011
identifier_str_mv MORAES, Vanessa Silva et al. Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni Worms. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 5, p. 600-604, 2013.
0074-0276
10.1590/0074-0276108052013011
url https://www.arca.fiocruz.br/handle/icict/59432
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fiocruz/IOC
publisher.none.fl_str_mv Fiocruz/IOC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/59432/2/0074-0276-mioc-108-05-600.pdf
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