Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest

Detalhes bibliográficos
Autor(a) principal: Melo, Caroline Vilas Boas de
Data de Publicação: 2021
Outros Autores: Torres, Felipe Guimarães, Hermida, Micely D’El-Rei, Fontes, Jonathan L. M., Mesquita, Bianca Ramos, Brito, Reginaldo, Ramos, Pablo Ivan P., Fernandes, Gabriel R., Freitas, Luiz Antônio Rodrigues, Khouri, Antonio Ricardo, Costa, Carlos Henrique Nery, dos-Santos, Washington Luis Conrado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50425
Resumo: Conselho Nacional deDesenvolvimento Cientı́fico e Tecnológico – CNPq (grant no. 400905/2013-2) and the research productivity scholarship provided to WS. We are also thankful for financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior – CAPES (grant no. 2072_2013) and the Fundação de Amparo à Pesquisa do Estado da Bahia – FAPESB (no. PET0053_2013).
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spelling Melo, Caroline Vilas Boas deTorres, Felipe GuimarãesHermida, Micely D’El-ReiFontes, Jonathan L. M.Mesquita, Bianca RamosBrito, ReginaldoRamos, Pablo Ivan P.Fernandes, Gabriel R.Freitas, Luiz Antônio RodriguesKhouri, Antonio RicardoCosta, Carlos Henrique Nerydos-Santos, Washington Luis Conrado2021-12-14T18:22:02Z2021-12-14T18:22:02Z2021MELO, Caroline Vilas Boas de et al. Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest. Frontiers in Immunology, 2021.1664-3224https://www.arca.fiocruz.br/handle/icict/5042510.3389/fimmu.2021.716314Conselho Nacional deDesenvolvimento Cientı́fico e Tecnológico – CNPq (grant no. 400905/2013-2) and the research productivity scholarship provided to WS. We are also thankful for financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior – CAPES (grant no. 2072_2013) and the Fundação de Amparo à Pesquisa do Estado da Bahia – FAPESB (no. PET0053_2013).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Enfermidades Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Centro de Integração de Dados e Conhecimento para Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Rene´ Rachou. Informática de Biossistemas. Minas Gerais, BH, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Enfermidades Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil.Universidade Federal do Piauí. Instituto de Doenças Tropicais Natan Portella. Departamento de Medicina Comunitária. Teresina, PI, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Structural changes in the spleen have been reported in several infectious diseases. In visceral leishmaniasis (VL), a severe parasitic disease caused by Leishmania spp., the loss of white pulp accompanies a severe clinical presentation. Hamster model reproduces aspects of human VL progression. In the early stages, a transcriptomic signature of leukocyte recruitment was associated with white pulp hyperplasia. Subsequently, impaired leukocyte chemotaxis with loss of T lymphocytes in the periarteriolar lymphoid sheath occurred. This differential gene expression was subsequently corroborated by transcriptomic profiling of spleens in severe human VL. At the latest stage, spleen disorganization was associated with increasing clinical signs of VL. White pulp disruption was accompanied by decreased DLK1 expression. The expression of CXCL13, CCR5, CCL19, CCR6, CCR7 and LTA decreased, likely regulated by CDKN2A overexpression. Our findings enlighten a pathway implying cell cycle arrest and decreased gene expression involved in spleen organization.engFrontiers MediaLeishmaniose visceralLeucócitosBaçoRNA-SeqBaço patologiaHiperplasiaVisceral leishmanaisisWhite pulp remodelingSpleen disorganizationTranscriptomic (RNA-Seq)Spleen pathology, hamsterSplenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrestinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/50425/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALMelo, Caroline Vilas Boas Splenic....pdfMelo, Caroline Vilas Boas Splenic....pdfapplication/pdf39628867https://www.arca.fiocruz.br/bitstream/icict/50425/2/Melo%2c%20Caroline%20Vilas%20Boas%20Splenic....pdf42e58e3596211ff13577c208f37968baMD52icict/504252023-03-15 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dc.title.pt_BR.fl_str_mv Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest
title Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest
spellingShingle Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest
Melo, Caroline Vilas Boas de
Leishmaniose visceral
Leucócitos
Baço
RNA-Seq
Baço patologia
Hiperplasia
Visceral leishmanaisis
White pulp remodeling
Spleen disorganization
Transcriptomic (RNA-Seq)
Spleen pathology, hamster
title_short Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest
title_full Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest
title_fullStr Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest
title_full_unstemmed Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest
title_sort Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest
author Melo, Caroline Vilas Boas de
author_facet Melo, Caroline Vilas Boas de
Torres, Felipe Guimarães
Hermida, Micely D’El-Rei
Fontes, Jonathan L. M.
Mesquita, Bianca Ramos
Brito, Reginaldo
Ramos, Pablo Ivan P.
Fernandes, Gabriel R.
Freitas, Luiz Antônio Rodrigues
Khouri, Antonio Ricardo
Costa, Carlos Henrique Nery
dos-Santos, Washington Luis Conrado
author_role author
author2 Torres, Felipe Guimarães
Hermida, Micely D’El-Rei
Fontes, Jonathan L. M.
Mesquita, Bianca Ramos
Brito, Reginaldo
Ramos, Pablo Ivan P.
Fernandes, Gabriel R.
Freitas, Luiz Antônio Rodrigues
Khouri, Antonio Ricardo
Costa, Carlos Henrique Nery
dos-Santos, Washington Luis Conrado
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Melo, Caroline Vilas Boas de
Torres, Felipe Guimarães
Hermida, Micely D’El-Rei
Fontes, Jonathan L. M.
Mesquita, Bianca Ramos
Brito, Reginaldo
Ramos, Pablo Ivan P.
Fernandes, Gabriel R.
Freitas, Luiz Antônio Rodrigues
Khouri, Antonio Ricardo
Costa, Carlos Henrique Nery
dos-Santos, Washington Luis Conrado
dc.subject.other.pt_BR.fl_str_mv Leishmaniose visceral
Leucócitos
Baço
RNA-Seq
Baço patologia
Hiperplasia
topic Leishmaniose visceral
Leucócitos
Baço
RNA-Seq
Baço patologia
Hiperplasia
Visceral leishmanaisis
White pulp remodeling
Spleen disorganization
Transcriptomic (RNA-Seq)
Spleen pathology, hamster
dc.subject.en.pt_BR.fl_str_mv Visceral leishmanaisis
White pulp remodeling
Spleen disorganization
Transcriptomic (RNA-Seq)
Spleen pathology, hamster
description Conselho Nacional deDesenvolvimento Cientı́fico e Tecnológico – CNPq (grant no. 400905/2013-2) and the research productivity scholarship provided to WS. We are also thankful for financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior – CAPES (grant no. 2072_2013) and the Fundação de Amparo à Pesquisa do Estado da Bahia – FAPESB (no. PET0053_2013).
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-12-14T18:22:02Z
dc.date.available.fl_str_mv 2021-12-14T18:22:02Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MELO, Caroline Vilas Boas de et al. Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest. Frontiers in Immunology, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50425
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
dc.identifier.eissn.none.fl_str_mv 10.3389/fimmu.2021.716314
identifier_str_mv MELO, Caroline Vilas Boas de et al. Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest. Frontiers in Immunology, 2021.
1664-3224
10.3389/fimmu.2021.716314
url https://www.arca.fiocruz.br/handle/icict/50425
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Frontiers Media
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bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50425/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50425/2/Melo%2c%20Caroline%20Vilas%20Boas%20Splenic....pdf
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