Phenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/41854 |
Resumo: | Research Ethics Committee of Instituto Gonçalo Moniz (IGM-FIOCRUZ, license nos. 004/2013 and 017/2015) |
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Melo, Caroline Vilas Boas deHermida, Micely D'El-ReiMesquita, Bianca RFontes, Jonathan L MKoning, Jasper JSolcà, Manuela da SilvaBenevides, Bruno BMota, Girlândia B SFreitas, Luiz Antonio Rodrigues deMebius, Reina EdosSantos, Washington Luis Conrado2020-06-24T17:23:04Z2020-06-24T17:23:04Z2020MELO, Caroline Vilas Boas de et al. Phenotypical characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis. Frontiers in Immunology, abr. 2020.1664-3224https://www.arca.fiocruz.br/handle/icict/4185410.3389/fimmu.2020.00653Research Ethics Committee of Instituto Gonçalo Moniz (IGM-FIOCRUZ, license nos. 004/2013 and 017/2015)Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.University Medical Center. Amsterdam Infection and Immunity Institute. Department of Molecular Cell Biology and Immunology. Amsterdam, Netherlands.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.University Medical Center. Amsterdam Infection and Immunity Institute. Department of Molecular Cell Biology and Immunology. Amsterdam, Netherlands.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Visceral leishmaniasis (VL) is caused by Leishmania infantum or L. donovani infection. One of the main problems related to this disease is the emergence of severe clinical forms with a lethality of 5-20%, even while under specific treatment. In humans and other species susceptible to fatal VL, such as dogs and hamsters, the disruption of splenic white pulp (WP) is accompanied by disease progression. Control of VL progression is seen in BALB/c mice, as evidenced by a mild clinical presentation and controlled parasite replication in the liver and spleen. In this study, we investigated the features involved in the morphological remodeling of splenic compartments associated with the control of VL progression to death. Methods: We evaluated cohorts of BALB/c mice after 30, 60, and 90 days of infection by L. infantum. Spleen morphology, cell population subsets and cytokine production were studied in the spleen using flow- and histo-cytometry. Results: Intraperitoneal infection with 108 promastigotes of L. infantum led to progressive increases in spleen size at 60 and 90 days after infection. Splenomegaly was the only clinical sign of disease observed. At 30 days after infection, hyperplasia in the WP and decreased numbers of plasmacytoid dendritic cells were observed. The WP hyperplasia subsided at 60 days post-infection. However, the splenomegaly remained in association with increased numbers of macrophages, B and T lymphocytes and plasma cells. An increased number of lymphoid tissue inducer (LTi) cells was observed; these were distributed around the periarteriolar lymphoid sheath in control mice and scattered throughout the red pulp in the Leishmania-infected mice. After 90 days of infection, increased IL-6 and IFN-γ production was seen in the spleen, as well as higher frequencies of follicular and plasmacytoid dendritic cells. Conclusion: The data presented herein emphasizes the potential role of spleen remodeling in the control of severe forms of VL and highlights features potentially involved in this process.engFrontiers MediaLeishmaniose VisceralRemodelaçãoBaçoTecido linfóidePatologia do baçoVisceral leishmaniasisWhite pulp remodelingSpleen disorganizationLymphoid tissue inducer cellsSpleen pathologyPhenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41854/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMelo, C. V.B. Phenotipical....pdfMelo, C. V.B. Phenotipical....pdfapplication/pdf2524726https://www.arca.fiocruz.br/bitstream/icict/41854/2/Melo%2c%20C.%20V.B.%20Phenotipical....pdff942d8cc0b18f5541c2b94bf63d6745eMD52TEXTMelo, C. V.B. Phenotipical....pdf.txtMelo, C. V.B. Phenotipical....pdf.txtExtracted texttext/plain54368https://www.arca.fiocruz.br/bitstream/icict/41854/3/Melo%2c%20C.%20V.B.%20Phenotipical....pdf.txtc520b16549d9b7e5bf06910f25013315MD53icict/418542023-03-15 14:34:25.816oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Phenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis |
| title |
Phenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis |
| spellingShingle |
Phenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis Melo, Caroline Vilas Boas de Leishmaniose Visceral Remodelação Baço Tecido linfóide Patologia do baço Visceral leishmaniasis White pulp remodeling Spleen disorganization Lymphoid tissue inducer cells Spleen pathology |
| title_short |
Phenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis |
| title_full |
Phenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis |
| title_fullStr |
Phenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis |
| title_full_unstemmed |
Phenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis |
| title_sort |
Phenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis |
| author |
Melo, Caroline Vilas Boas de |
| author_facet |
Melo, Caroline Vilas Boas de Hermida, Micely D'El-Rei Mesquita, Bianca R Fontes, Jonathan L M Koning, Jasper J Solcà, Manuela da Silva Benevides, Bruno B Mota, Girlândia B S Freitas, Luiz Antonio Rodrigues de Mebius, Reina E dosSantos, Washington Luis Conrado |
| author_role |
author |
| author2 |
Hermida, Micely D'El-Rei Mesquita, Bianca R Fontes, Jonathan L M Koning, Jasper J Solcà, Manuela da Silva Benevides, Bruno B Mota, Girlândia B S Freitas, Luiz Antonio Rodrigues de Mebius, Reina E dosSantos, Washington Luis Conrado |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Melo, Caroline Vilas Boas de Hermida, Micely D'El-Rei Mesquita, Bianca R Fontes, Jonathan L M Koning, Jasper J Solcà, Manuela da Silva Benevides, Bruno B Mota, Girlândia B S Freitas, Luiz Antonio Rodrigues de Mebius, Reina E dosSantos, Washington Luis Conrado |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose Visceral Remodelação Baço Tecido linfóide Patologia do baço |
| topic |
Leishmaniose Visceral Remodelação Baço Tecido linfóide Patologia do baço Visceral leishmaniasis White pulp remodeling Spleen disorganization Lymphoid tissue inducer cells Spleen pathology |
| dc.subject.en.pt_BR.fl_str_mv |
Visceral leishmaniasis White pulp remodeling Spleen disorganization Lymphoid tissue inducer cells Spleen pathology |
| description |
Research Ethics Committee of Instituto Gonçalo Moniz (IGM-FIOCRUZ, license nos. 004/2013 and 017/2015) |
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2020 |
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2020-06-24T17:23:04Z |
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2020-06-24T17:23:04Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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MELO, Caroline Vilas Boas de et al. Phenotypical characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis. Frontiers in Immunology, abr. 2020. |
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https://www.arca.fiocruz.br/handle/icict/41854 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2020.00653 |
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MELO, Caroline Vilas Boas de et al. Phenotypical characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis. Frontiers in Immunology, abr. 2020. 1664-3224 10.3389/fimmu.2020.00653 |
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