Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations

Detalhes bibliográficos
Autor(a) principal: Moreira, Douglas de Souza
Data de Publicação: 2018
Outros Autores: Duarte, Ana Paula, Pais, Fabiano Sviatopolk Mirsky, Pereira, Rosiane Aparecida da Silva, Romanha, Alvaro José, Schenkman, Sergio, Murta, Silvane Maria Fonseca
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32939
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
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spelling Moreira, Douglas de SouzaDuarte, Ana PaulaPais, Fabiano Sviatopolk MirskyPereira, Rosiane Aparecida da SilvaRomanha, Alvaro JoséSchenkman, SergioMurta, Silvane Maria Fonseca2019-05-06T12:19:13Z2019-05-06T12:19:13Z2018MOREIRA, Douglas de Souza et al. Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 9, p. 1-5, 2018.0074-0276https://www.arca.fiocruz.br/handle/icict/3293910.1590/0074-02760180162engFundação Oswaldo CruzTrypanosoma cruziSusceptibilidadeResistênciaTrypanosoma cruziDrug resistanceBenznidazoleEukaryotic initiation factor 5A (eIF5A)Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Eukaryotic initiation factor 5A (eIF5A) is a conserved protein with an essential role in translation elongation. Using one and two-dimensional western blotting, we showed that the eIF5A protein level was 2-fold lower in benznidazole (BZ)-resistant (BZR and 17LER) Trypanosoma cruzi populations than in their respective susceptible counterparts (BZS and 17WTS). To confirm the role of eIF5A in BZ resistance, we transfected BZS and 17WTS with the wild-type eIF5A or mutant eIF5A-S2A (in which serine 2 was replaced by alanine). Upon overexpressing eIF5A, both susceptible lines became approximately 3- and 5-fold more sensitive to BZ. In contrast, the eIF5A-S2A mutant did not alter its susceptibility to BZ. These data suggest that BZ resistance might arise from either decreasing the translation of proteins that require eIF5A, or as a consequence of differential levels of precursors for the hypusination reactions (e.g., spermidine and trypanothione), both of which alter BZ’s effects in the parasite.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/32939/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALOverexpression of eukaryotic initiation .pdfOverexpression of eukaryotic initiation .pdfapplication/pdf5219171https://www.arca.fiocruz.br/bitstream/icict/32939/2/Overexpression%20of%20eukaryotic%20initiation%20.pdf3c5ac18003adf1d210c2c5cb05ef4af4MD52TEXTOverexpression of eukaryotic initiation .pdf.txtOverexpression of eukaryotic initiation .pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations
title Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations
spellingShingle Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations
Moreira, Douglas de Souza
Trypanosoma cruzi
Susceptibilidade
Resistência
Trypanosoma cruzi
Drug resistance
Benznidazole
Eukaryotic initiation factor 5A (eIF5A)
title_short Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations
title_full Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations
title_fullStr Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations
title_full_unstemmed Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations
title_sort Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations
author Moreira, Douglas de Souza
author_facet Moreira, Douglas de Souza
Duarte, Ana Paula
Pais, Fabiano Sviatopolk Mirsky
Pereira, Rosiane Aparecida da Silva
Romanha, Alvaro José
Schenkman, Sergio
Murta, Silvane Maria Fonseca
author_role author
author2 Duarte, Ana Paula
Pais, Fabiano Sviatopolk Mirsky
Pereira, Rosiane Aparecida da Silva
Romanha, Alvaro José
Schenkman, Sergio
Murta, Silvane Maria Fonseca
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moreira, Douglas de Souza
Duarte, Ana Paula
Pais, Fabiano Sviatopolk Mirsky
Pereira, Rosiane Aparecida da Silva
Romanha, Alvaro José
Schenkman, Sergio
Murta, Silvane Maria Fonseca
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Susceptibilidade
Resistência
topic Trypanosoma cruzi
Susceptibilidade
Resistência
Trypanosoma cruzi
Drug resistance
Benznidazole
Eukaryotic initiation factor 5A (eIF5A)
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Drug resistance
Benznidazole
Eukaryotic initiation factor 5A (eIF5A)
description Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-05-06T12:19:13Z
dc.date.available.fl_str_mv 2019-05-06T12:19:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOREIRA, Douglas de Souza et al. Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 9, p. 1-5, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32939
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760180162
identifier_str_mv MOREIRA, Douglas de Souza et al. Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 9, p. 1-5, 2018.
0074-0276
10.1590/0074-02760180162
url https://www.arca.fiocruz.br/handle/icict/32939
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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