Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/32939 |
Resumo: | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. |
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Moreira, Douglas de SouzaDuarte, Ana PaulaPais, Fabiano Sviatopolk MirskyPereira, Rosiane Aparecida da SilvaRomanha, Alvaro JoséSchenkman, SergioMurta, Silvane Maria Fonseca2019-05-06T12:19:13Z2019-05-06T12:19:13Z2018MOREIRA, Douglas de Souza et al. Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 9, p. 1-5, 2018.0074-0276https://www.arca.fiocruz.br/handle/icict/3293910.1590/0074-02760180162engFundação Oswaldo CruzTrypanosoma cruziSusceptibilidadeResistênciaTrypanosoma cruziDrug resistanceBenznidazoleEukaryotic initiation factor 5A (eIF5A)Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Eukaryotic initiation factor 5A (eIF5A) is a conserved protein with an essential role in translation elongation. Using one and two-dimensional western blotting, we showed that the eIF5A protein level was 2-fold lower in benznidazole (BZ)-resistant (BZR and 17LER) Trypanosoma cruzi populations than in their respective susceptible counterparts (BZS and 17WTS). To confirm the role of eIF5A in BZ resistance, we transfected BZS and 17WTS with the wild-type eIF5A or mutant eIF5A-S2A (in which serine 2 was replaced by alanine). Upon overexpressing eIF5A, both susceptible lines became approximately 3- and 5-fold more sensitive to BZ. In contrast, the eIF5A-S2A mutant did not alter its susceptibility to BZ. These data suggest that BZ resistance might arise from either decreasing the translation of proteins that require eIF5A, or as a consequence of differential levels of precursors for the hypusination reactions (e.g., spermidine and trypanothione), both of which alter BZ’s effects in the parasite.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/32939/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALOverexpression of eukaryotic initiation .pdfOverexpression of eukaryotic initiation .pdfapplication/pdf5219171https://www.arca.fiocruz.br/bitstream/icict/32939/2/Overexpression%20of%20eukaryotic%20initiation%20.pdf3c5ac18003adf1d210c2c5cb05ef4af4MD52TEXTOverexpression of eukaryotic initiation .pdf.txtOverexpression of eukaryotic initiation .pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations |
title |
Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations |
spellingShingle |
Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations Moreira, Douglas de Souza Trypanosoma cruzi Susceptibilidade Resistência Trypanosoma cruzi Drug resistance Benznidazole Eukaryotic initiation factor 5A (eIF5A) |
title_short |
Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations |
title_full |
Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations |
title_fullStr |
Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations |
title_full_unstemmed |
Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations |
title_sort |
Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations |
author |
Moreira, Douglas de Souza |
author_facet |
Moreira, Douglas de Souza Duarte, Ana Paula Pais, Fabiano Sviatopolk Mirsky Pereira, Rosiane Aparecida da Silva Romanha, Alvaro José Schenkman, Sergio Murta, Silvane Maria Fonseca |
author_role |
author |
author2 |
Duarte, Ana Paula Pais, Fabiano Sviatopolk Mirsky Pereira, Rosiane Aparecida da Silva Romanha, Alvaro José Schenkman, Sergio Murta, Silvane Maria Fonseca |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Moreira, Douglas de Souza Duarte, Ana Paula Pais, Fabiano Sviatopolk Mirsky Pereira, Rosiane Aparecida da Silva Romanha, Alvaro José Schenkman, Sergio Murta, Silvane Maria Fonseca |
dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi Susceptibilidade Resistência |
topic |
Trypanosoma cruzi Susceptibilidade Resistência Trypanosoma cruzi Drug resistance Benznidazole Eukaryotic initiation factor 5A (eIF5A) |
dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Drug resistance Benznidazole Eukaryotic initiation factor 5A (eIF5A) |
description |
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-05-06T12:19:13Z |
dc.date.available.fl_str_mv |
2019-05-06T12:19:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MOREIRA, Douglas de Souza et al. Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 9, p. 1-5, 2018. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/32939 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
10.1590/0074-02760180162 |
identifier_str_mv |
MOREIRA, Douglas de Souza et al. Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 9, p. 1-5, 2018. 0074-0276 10.1590/0074-02760180162 |
url |
https://www.arca.fiocruz.br/handle/icict/32939 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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Fundação Oswaldo Cruz |
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Fundação Oswaldo Cruz |
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