Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/33135 |
Resumo: | Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil. |
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Pires, Weverson LucianoCastro, Onassis Boeri deKayano, Anderson MakotoSetúbal, Sulamita da SilvaPontes, Adriana SilvaNery, Neriane MonteiroPaloschi, Mauro ValentinoPereira, Soraya dos SantosStábeli, Rodrigo GuerinoFernandes, Carla Freire CeledônioSoares, Andreimar MartinsZuliani, Juliana Pavan2019-05-17T20:51:17Z2019-05-17T20:51:17Z2017PIRES, Weverson Luciano et al. Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells. Toxicology in Vitro, v. 41, p. 30-41, 2017.0887-2333https://www.arca.fiocruz.br/handle/icict/3313510.1016/j.tiv.2017.02.0031879-3177engElsevierEffect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil. / Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Engenharia de Anticorpos. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Engenharia de Anticorpos. Porto Velho, RO, Brasil. / Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil. / Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.BjcuL is a C-type lectin with specificity for the binding of β-D-galactose units isolated from Bothrops jararacussu venom. It triggers cellular infiltration in post capillary venules, increases edema and vascular permeability inmurine models, contributes to in vitro neutrophil activation and modulates macrophage functional activation towards an M1 state. The purpose of this study was to investigate the effect of BjcuL on human peripheral blood mononuclear cells (PBMCs) activation with a focus on PBMCs proliferation and inflammatory mediators release. Results showed that BjcuL is not toxic to PBMCs, that BjcuL inhibits PBMCs proliferation and that it stimulates PBMCs to produce superoxide anion and hydrogen peroxide, primarily via lymphocyte stimulation, but does not stimulate the production of nitric oxide and PGE2. These results demonstrate that BjcuL has an immunomodulatory effect on PBMCs. Further studies are needed to confirm the immunomodulatory effect of BjcuL, to elucidate the molecular mechanisms of action responsible for its effects and to determine its potential application as an immunopharmacological and biotechnological tool.Snake venomLectinPBMCsMediatorsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/33135/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEffect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells.pdfEffect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells.pdfapplication/pdf1969117https://www.arca.fiocruz.br/bitstream/icict/33135/2/Effect%20of%20BjcuL%2c%20a%20lectin%20isolated%20from%20Bothrops%20jararacussu%2c%20on%20human%20peripheral%20blood%20mononuclear%20cells.pdf83d69880c202052f691a3820748335b6MD52TEXTEffect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells.pdf.txtEffect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells.pdf.txtExtracted texttext/plain54847https://www.arca.fiocruz.br/bitstream/icict/33135/3/Effect%20of%20BjcuL%2c%20a%20lectin%20isolated%20from%20Bothrops%20jararacussu%2c%20on%20human%20peripheral%20blood%20mononuclear%20cells.pdf.txt1645afae7b536499150025e9b2aa514fMD53icict/331352019-05-18 16:32:31.342oai:www.arca.fiocruz.br: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ório 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| dc.title.pt_BR.fl_str_mv |
Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells |
| title |
Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells |
| spellingShingle |
Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells Pires, Weverson Luciano Snake venom Lectin PBMCs Mediators |
| title_short |
Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells |
| title_full |
Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells |
| title_fullStr |
Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells |
| title_full_unstemmed |
Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells |
| title_sort |
Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells |
| author |
Pires, Weverson Luciano |
| author_facet |
Pires, Weverson Luciano Castro, Onassis Boeri de Kayano, Anderson Makoto Setúbal, Sulamita da Silva Pontes, Adriana Silva Nery, Neriane Monteiro Paloschi, Mauro Valentino Pereira, Soraya dos Santos Stábeli, Rodrigo Guerino Fernandes, Carla Freire Celedônio Soares, Andreimar Martins Zuliani, Juliana Pavan |
| author_role |
author |
| author2 |
Castro, Onassis Boeri de Kayano, Anderson Makoto Setúbal, Sulamita da Silva Pontes, Adriana Silva Nery, Neriane Monteiro Paloschi, Mauro Valentino Pereira, Soraya dos Santos Stábeli, Rodrigo Guerino Fernandes, Carla Freire Celedônio Soares, Andreimar Martins Zuliani, Juliana Pavan |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pires, Weverson Luciano Castro, Onassis Boeri de Kayano, Anderson Makoto Setúbal, Sulamita da Silva Pontes, Adriana Silva Nery, Neriane Monteiro Paloschi, Mauro Valentino Pereira, Soraya dos Santos Stábeli, Rodrigo Guerino Fernandes, Carla Freire Celedônio Soares, Andreimar Martins Zuliani, Juliana Pavan |
| dc.subject.en.pt_BR.fl_str_mv |
Snake venom Lectin PBMCs Mediators |
| topic |
Snake venom Lectin PBMCs Mediators |
| description |
Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil. |
| publishDate |
2017 |
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2017 |
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2019-05-17T20:51:17Z |
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2019-05-17T20:51:17Z |
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PIRES, Weverson Luciano et al. Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells. Toxicology in Vitro, v. 41, p. 30-41, 2017. |
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https://www.arca.fiocruz.br/handle/icict/33135 |
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0887-2333 |
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10.1016/j.tiv.2017.02.003 |
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1879-3177 |
| identifier_str_mv |
PIRES, Weverson Luciano et al. Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells. Toxicology in Vitro, v. 41, p. 30-41, 2017. 0887-2333 10.1016/j.tiv.2017.02.003 1879-3177 |
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