Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.

Detalhes bibliográficos
Autor(a) principal: Pereira, Roberta Verciano
Data de Publicação: 2015
Outros Autores: Gomes, Matheus de Souza, Olmo, Roenick Proveti, Souza, Daniel M., Cabral, Fernanda J., Passos, Liana konovaloff Jannotti, Baba, Elio Hideo, Andreolli, Andressa B. P., Rodrigues, Vanderlei, Borges, William Castro, Sá, Renata Guerra
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12742
Resumo: Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brasil
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spelling Pereira, Roberta VercianoGomes, Matheus de SouzaOlmo, Roenick ProvetiSouza, Daniel M.Cabral, Fernanda J.Passos, Liana konovaloff JannottiBaba, Elio HideoAndreolli, Andressa B. P.Rodrigues, VanderleiBorges, William CastroSá, Renata Guerra2016-02-16T18:15:37Z2016-02-16T18:15:37Z2015PEREIRA, Roberta Verciano et al. Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle. Parasites & Vectors, vol. 8, p. 349, 20151756-3305https://www.arca.fiocruz.br/handle/icict/1274210.1186/s13071-015-0957-4porSpringerUbiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Patos de Minas, MG, Brasil.Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilInstituto de Ciências Biomédicas Butantã.. Departamento de Fisiologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. São Paulo, Brasil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. São Paulo, Brasil.Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brasil/Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Departamento de Ciências Biológicas. Ouro Preto, MG, Brasil.Background: The ubiquitination process can be reversed by deubiquitinating enzymes (DUBs). These proteases are involved in ubiquitin processing, in the recovery of modified ubiquitin trapped in inactive forms, and in the recycling of ubiquitin monomers from polyubiquitinated chains. The diversity of DUB functions is illustrated by their number and variety of their catalytic domains with specific 3D architectures. DUBs can be divided into five subclasses: ubiquitin C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs or UBPs), ovarian tumour proteases (OTUs), Machado-Joseph disease proteases (MJDs) and JAB1/MPN/Mov34 metalloenzymes (JAMMs). Methods: Considering the role that the ubiquitin-proteasome system has been shown to play during the development ofSchistosoma mansoni, our main goal was to identify and characterize SmUSPs. Here, we showed the identification of putative ubiquitin-specific proteases using bioinformatic approaches. We also evaluated the gene expression profile of representative USP family members using qRT-PCR. Results: We reported 17 USP family members in S. mansoni that present a conservation of UCH domains. Furthermore, the putativeSmUSP transcripts analysed were detected in all investigated stages, showing distinct expression during S. mansonidevelopment. The SmUSPs exhibiting high expression profiles were SmUSP7, SmUSP8, SmUSP9x and SmUSP24. Conclusion: S. mansoni USPs showed changes in expression levels for different life cycle stages indicating their involvement in cellular processes required for S. mansoni development. These data will serve as a basis for future functional studies of USPs in this parasite.UbiquitinationeubiquitinationDDeubiquitinating enzymes (DUBs)Differential expressionSchistosome developmentinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12742/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALUbiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.pdfUbiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.pdfapplication/pdf10854490https://www.arca.fiocruz.br/bitstream/icict/12742/2/Ubiquitin-specific%20proteases%20are%20differentially%20expressed%20throughout%20the%20Schistosoma%20mansoni%20life%20cycle.pdfea309d46076c53adba1eaaafffeccdb4MD52TEXTUbiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.pdf.txtUbiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.pdf.txtExtracted texttext/plain11https://www.arca.fiocruz.br/bitstream/icict/12742/3/Ubiquitin-specific%20proteases%20are%20differentially%20expressed%20throughout%20the%20Schistosoma%20mansoni%20life%20cycle.pdf.txtb308b7fe5c1c2bbdc0cb686d451b84aaMD53icict/127422022-02-01 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dc.title.pt_BR.fl_str_mv Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.
title Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.
spellingShingle Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.
Pereira, Roberta Verciano
Ubiquitination
eubiquitination
DDeubiquitinating enzymes (DUBs)
Differential expression
Schistosome development
title_short Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.
title_full Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.
title_fullStr Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.
title_full_unstemmed Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.
title_sort Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle.
author Pereira, Roberta Verciano
author_facet Pereira, Roberta Verciano
Gomes, Matheus de Souza
Olmo, Roenick Proveti
Souza, Daniel M.
Cabral, Fernanda J.
Passos, Liana konovaloff Jannotti
Baba, Elio Hideo
Andreolli, Andressa B. P.
Rodrigues, Vanderlei
Borges, William Castro
Sá, Renata Guerra
author_role author
author2 Gomes, Matheus de Souza
Olmo, Roenick Proveti
Souza, Daniel M.
Cabral, Fernanda J.
Passos, Liana konovaloff Jannotti
Baba, Elio Hideo
Andreolli, Andressa B. P.
Rodrigues, Vanderlei
Borges, William Castro
Sá, Renata Guerra
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Roberta Verciano
Gomes, Matheus de Souza
Olmo, Roenick Proveti
Souza, Daniel M.
Cabral, Fernanda J.
Passos, Liana konovaloff Jannotti
Baba, Elio Hideo
Andreolli, Andressa B. P.
Rodrigues, Vanderlei
Borges, William Castro
Sá, Renata Guerra
dc.subject.en.pt_BR.fl_str_mv Ubiquitination
eubiquitination
DDeubiquitinating enzymes (DUBs)
Differential expression
Schistosome development
topic Ubiquitination
eubiquitination
DDeubiquitinating enzymes (DUBs)
Differential expression
Schistosome development
description Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-02-16T18:15:37Z
dc.date.available.fl_str_mv 2016-02-16T18:15:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, Roberta Verciano et al. Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle. Parasites & Vectors, vol. 8, p. 349, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12742
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-015-0957-4
identifier_str_mv PEREIRA, Roberta Verciano et al. Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle. Parasites & Vectors, vol. 8, p. 349, 2015
1756-3305
10.1186/s13071-015-0957-4
url https://www.arca.fiocruz.br/handle/icict/12742
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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