In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.

Detalhes bibliográficos
Autor(a) principal: Costa, Marcela Pereira
Data de Publicação: 2015
Outros Autores: Oliveira, Victor Fernandes de, Pereira, Roberta Verciano, Abreu, Fabiano Carlos Pinto de, Passos, Liana konovaloff Jannotti, Borges, William de Castro, Cota, Renata Guerra de Sá
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15742
Resumo: 2019-01-01
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spelling Costa, Marcela PereiraOliveira, Victor Fernandes dePereira, Roberta VercianoAbreu, Fabiano Carlos Pinto dePassos, Liana konovaloff JannottiBorges, William de CastroCota, Renata Guerra de Sá2016-09-09T14:26:51Z2016-09-09T14:26:51Z2015COSTA, Marcela Pereira et al. In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni. Parasitol Res, v. 114, p. 1769–1777, 20150932-0113https://www.arca.fiocruz.br/handle/icict/15742DOI 10.1007/s00436-015-4362-xengSpringer Berlin HeidelbergIn silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2019-01-01Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Fiocruz, Belo Horizonte, MG, BrasilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUbiquitin-conjugating enzymes (Ub-E2) perform the second step of ubiquitination and, consequently, are essential for regulating proteolysis and for modulating protein function, interactions and trafficking. Previously, our group demonstrated the crucial role of ubiquitination and the Ubproteasome pathway during the Schistosoma mansoni life cycle. In the present investigation, we used a homology-based genome-wide bioinformatics approach to identify and molecularly characterise the Ub-E2 enzymes in S. mansoni. The putative functions were further investigated through molecular phylogenetic and expression profile analyses using cercariae, adult worms, eggs and mechanically transformed schistosomula (MTS) cultured in vitro for 3.5 h or 1 or 3 days. We identified, via in silico analysis, 17 Ub-E2 enzymes with conserved structural characteristics: the beta-sheet and the helix-2 form a central core bordered by helix-1 at one side and helix-3 and helix-4 at the other. The observed quantitative differences in the steady-state transcript levels between the cercariae and adult worms may contribute to the differential protein ubiquitination observed during the parasite ’s life cycle. This study is the first to identify and characterise the E2 ubiquitin conjugation family in S. mansoni and provides fundamental information regarding their molecular phylogenetics and developmental expression during intra-mammalian stages.Schistosoma mansoniUbiquitinationUbiquitin-conjugating enzymeE2Differential expressioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15742/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Marcela_Costa_InSilico_CPqRR_2015.pdfve_Marcela_Costa_InSilico_CPqRR_2015.pdfapplication/pdf7208171https://www.arca.fiocruz.br/bitstream/icict/15742/2/ve_Marcela_Costa_InSilico_CPqRR_2015.pdf9f3c630a6faed5ed3e47376377c7af8cMD52TEXTve_Marcela_Costa_InSilico_CPqRR_2015.pdf.txtve_Marcela_Costa_InSilico_CPqRR_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.
title In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.
spellingShingle In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.
Costa, Marcela Pereira
Schistosoma mansoni
Ubiquitination
Ubiquitin-conjugating enzyme
E2
Differential expression
title_short In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.
title_full In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.
title_fullStr In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.
title_full_unstemmed In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.
title_sort In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.
author Costa, Marcela Pereira
author_facet Costa, Marcela Pereira
Oliveira, Victor Fernandes de
Pereira, Roberta Verciano
Abreu, Fabiano Carlos Pinto de
Passos, Liana konovaloff Jannotti
Borges, William de Castro
Cota, Renata Guerra de Sá
author_role author
author2 Oliveira, Victor Fernandes de
Pereira, Roberta Verciano
Abreu, Fabiano Carlos Pinto de
Passos, Liana konovaloff Jannotti
Borges, William de Castro
Cota, Renata Guerra de Sá
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Marcela Pereira
Oliveira, Victor Fernandes de
Pereira, Roberta Verciano
Abreu, Fabiano Carlos Pinto de
Passos, Liana konovaloff Jannotti
Borges, William de Castro
Cota, Renata Guerra de Sá
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
Ubiquitination
Ubiquitin-conjugating enzyme
E2
Differential expression
topic Schistosoma mansoni
Ubiquitination
Ubiquitin-conjugating enzyme
E2
Differential expression
description 2019-01-01
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-09-09T14:26:51Z
dc.date.available.fl_str_mv 2016-09-09T14:26:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Marcela Pereira et al. In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni. Parasitol Res, v. 114, p. 1769–1777, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15742
dc.identifier.issn.pt_BR.fl_str_mv 0932-0113
dc.identifier.doi.none.fl_str_mv DOI 10.1007/s00436-015-4362-x
identifier_str_mv COSTA, Marcela Pereira et al. In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni. Parasitol Res, v. 114, p. 1769–1777, 2015
0932-0113
DOI 10.1007/s00436-015-4362-x
url https://www.arca.fiocruz.br/handle/icict/15742
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Berlin Heidelberg
publisher.none.fl_str_mv Springer Berlin Heidelberg
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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