Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents

Detalhes bibliográficos
Autor(a) principal: Verissimo-Villela, Érika
Data de Publicação: 2016
Outros Autores: Kitahara-Oliveira, Yoko, Reis, Ana Beatriz de Bragança dos, Albano, Rodolpho Mattos, Da-Cruz, Alda Maria, Bello, Alexandre Ribeiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14631
Resumo: Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
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spelling Verissimo-Villela, ÉrikaKitahara-Oliveira, YokoReis, Ana Beatriz de Bragança dosAlbano, Rodolpho MattosDa-Cruz, Alda MariaBello, Alexandre Ribeiro2016-06-23T18:13:32Z2016-07-07T11:47:51Z2016-06-23T18:13:32Z2016-07-07T11:47:51Z2016VERÍSSIMO-VILLELA, Erika; et al. Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents. Mem Inst Oswaldo Cruz, v.111, n.5, p. 349-354, May 2016.0074-0276https://www.arca.fiocruz.br/handle/icict/1463110.1590/0074-027601504121678-8080engFundação Oswaldo CruzFunctional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agentsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Laboratório de Genoma. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.During its life cycle Leishmania spp. face several stress conditions that can cause DNA damages. Base Excision Repair plays an important role in DNA maintenance and it is one of the most conserved mechanisms in all living organisms. DNA repair in trypanosomatids has been reported only for Old World Leishmania species. Here the AP endonuclease from Leishmania (L.) amazonensis was cloned, expressed in Escherichia coli mutants defective on the DNA repair machinery, that were submitted to different stress conditions, showing ability to survive in comparison to the triple null mutant parental strain BW535. Phylogenetic and multiple sequence analyses also confirmed that LAMAP belongs to the AP endonuclease class of proteins.AP endonucleaseLeishmania amazonensislamapBase Excision RepairDNA repairReparo do DNAEndonucleoseLeishmaniainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTmilene_oliveira_etal_IOC_2016.pdf.txtmilene_oliveira_etal_IOC_2016.pdf.txtExtracted texttext/plain28934https://www.arca.fiocruz.br/bitstream/icict/14631/4/milene_oliveira_etal_IOC_2016.pdf.txtd36de3026603b493f09fedfb89ef99a0MD54ORIGINALmilene_oliveira_etal_IOC_2016.pdfapplication/pdf2331961https://www.arca.fiocruz.br/bitstream/icict/14631/2/milene_oliveira_etal_IOC_2016.pdf5fa155fc65b069851c1f52de01101bfdMD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14631/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/146312022-06-24 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dc.title.pt_BR.fl_str_mv Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
title Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
spellingShingle Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Verissimo-Villela, Érika
AP endonuclease
Leishmania amazonensis
lamap
Base Excision Repair
DNA repair
Reparo do DNA
Endonucleose
Leishmania
title_short Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
title_full Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
title_fullStr Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
title_full_unstemmed Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
title_sort Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
author Verissimo-Villela, Érika
author_facet Verissimo-Villela, Érika
Kitahara-Oliveira, Yoko
Reis, Ana Beatriz de Bragança dos
Albano, Rodolpho Mattos
Da-Cruz, Alda Maria
Bello, Alexandre Ribeiro
author_role author
author2 Kitahara-Oliveira, Yoko
Reis, Ana Beatriz de Bragança dos
Albano, Rodolpho Mattos
Da-Cruz, Alda Maria
Bello, Alexandre Ribeiro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Verissimo-Villela, Érika
Kitahara-Oliveira, Yoko
Reis, Ana Beatriz de Bragança dos
Albano, Rodolpho Mattos
Da-Cruz, Alda Maria
Bello, Alexandre Ribeiro
dc.subject.en.pt_BR.fl_str_mv AP endonuclease
Leishmania amazonensis
lamap
Base Excision Repair
DNA repair
topic AP endonuclease
Leishmania amazonensis
lamap
Base Excision Repair
DNA repair
Reparo do DNA
Endonucleose
Leishmania
dc.subject.decs.pt_BR.fl_str_mv Reparo do DNA
Endonucleose
Leishmania
description Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-06-23T18:13:32Z
2016-07-07T11:47:51Z
dc.date.available.fl_str_mv 2016-06-23T18:13:32Z
2016-07-07T11:47:51Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VERÍSSIMO-VILLELA, Erika; et al. Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents. Mem Inst Oswaldo Cruz, v.111, n.5, p. 349-354, May 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14631
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/0074-02760150412
dc.identifier.eissn.pt_BR.fl_str_mv 1678-8080
identifier_str_mv VERÍSSIMO-VILLELA, Erika; et al. Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents. Mem Inst Oswaldo Cruz, v.111, n.5, p. 349-354, May 2016.
0074-0276
10.1590/0074-02760150412
1678-8080
url https://www.arca.fiocruz.br/handle/icict/14631
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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