Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen

Detalhes bibliográficos
Autor(a) principal: Nogueira, Sarah Veloso
Data de Publicação: 2013
Outros Autores: Backstedt, Brian T, Smith, Alexis A, Qin, Jin-Hong, Wunder Junior, Elsio Augusto, Ko, Albert Icksang, Pal, Utpal
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20088
Resumo: Ko, Albert Icksang “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Nogueira, Sarah VelosoBackstedt, Brian TSmith, Alexis AQin, Jin-HongWunder Junior, Elsio AugustoKo, Albert IcksangPal, Utpal2017-07-14T14:11:43Z2017-07-14T14:11:43Z2013NOGUEIRA, S. V. et al. Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen. PLoS One, v. 8, n. 10, p. e78150, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/2008810.1371/journal.pone.0078150Ko, Albert Icksang “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.National Institutes of Health (AI080615, AI088752 and AI052473)University of Maryland. Virginia Maryland Regional College of Veterinary Medicine. Department of Veterinary Medicine. Maryland, USAUniversity of Maryland. Virginia Maryland Regional College of Veterinary Medicine. Department of Veterinary Medicine. Maryland, USAUniversity of Maryland. Virginia Maryland Regional College of Veterinary Medicine. Department of Veterinary Medicine. Maryland, USAUniversity of Maryland. Virginia Maryland Regional College of Veterinary Medicine. Department of Veterinary Medicine. Maryland, USAYale University School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, Connecticut, USAYale University School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, Connecticut, USAUniversity of Maryland. Virginia Maryland Regional College of Veterinary Medicine. Department of Veterinary Medicine. Maryland, USALeptospira interrogans is the agent for leptospirosis, an important zoonosis in humans and animals across the globe. Surface proteins of invading pathogens, such as L. interrogans, are thought to be responsible for successful microbial persistence in vivo via interaction with specific host components. In particular, a number of invasive infectious agents exploit host proteolytic pathways, such as one involving plasminogen (Pg), which aid in efficient pathogen dissemination within the host. Here we show that L. interrogans serovar Lai binds host Pg and that the leptospiral gene product LA1951, annotated as enolase, is involved in this interaction. Interestingly, unlike in related pathogenic Spirochetes, such as Borrelia burgdorferi, LA1951 is not readily detectable in the L. interrogans outer membrane. We show that the antigen is indeed secreted extracellularly; however, it can reassociate with the pathogen surface, where it displays Pg-binding and measurable enzymatic activity. Hamsters infected with L. interrogans also develop readily detectable antibody responses against enolase. Taken together, our results suggest that the L. interrogans enolase has evolved to play a role in pathogen interaction with host molecules, which may contribute to the pathogenesis of leptospirosis.engPublic Library of ScienceLeptospira interrogansProteínasPlasminogenioCricetinaeAnimaisLeptospira interrogansBacterial ProteinsPlasminogenCricetinaeAnimalsLeptospira interrogans enolase is secreted extracellularly and interacts with plasminogeninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20088/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALNogueira SV leptospira interrogans enolase....pdfNogueira SV leptospira interrogans 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dc.title.pt_BR.fl_str_mv Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen
title Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen
spellingShingle Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen
Nogueira, Sarah Veloso
Leptospira interrogans
Proteínas
Plasminogenio
Cricetinae
Animais
Leptospira interrogans
Bacterial Proteins
Plasminogen
Cricetinae
Animals
title_short Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen
title_full Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen
title_fullStr Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen
title_full_unstemmed Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen
title_sort Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen
author Nogueira, Sarah Veloso
author_facet Nogueira, Sarah Veloso
Backstedt, Brian T
Smith, Alexis A
Qin, Jin-Hong
Wunder Junior, Elsio Augusto
Ko, Albert Icksang
Pal, Utpal
author_role author
author2 Backstedt, Brian T
Smith, Alexis A
Qin, Jin-Hong
Wunder Junior, Elsio Augusto
Ko, Albert Icksang
Pal, Utpal
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nogueira, Sarah Veloso
Backstedt, Brian T
Smith, Alexis A
Qin, Jin-Hong
Wunder Junior, Elsio Augusto
Ko, Albert Icksang
Pal, Utpal
dc.subject.other.pt_BR.fl_str_mv Leptospira interrogans
Proteínas
Plasminogenio
Cricetinae
Animais
topic Leptospira interrogans
Proteínas
Plasminogenio
Cricetinae
Animais
Leptospira interrogans
Bacterial Proteins
Plasminogen
Cricetinae
Animals
dc.subject.en.pt_BR.fl_str_mv Leptospira interrogans
Bacterial Proteins
Plasminogen
Cricetinae
Animals
description Ko, Albert Icksang “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2017-07-14T14:11:43Z
dc.date.available.fl_str_mv 2017-07-14T14:11:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NOGUEIRA, S. V. et al. Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen. PLoS One, v. 8, n. 10, p. e78150, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20088
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0078150
identifier_str_mv NOGUEIRA, S. V. et al. Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen. PLoS One, v. 8, n. 10, p. e78150, 2013.
1932-6203
10.1371/journal.pone.0078150
url https://www.arca.fiocruz.br/handle/icict/20088
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/20088/3/Nogueira%20SV%20leptospira%20interrogans%20enolase....pdf.txt
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