Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20085 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Instituto Nacional de Ciência e Tecnologia (INCT) de Informação Genético-Sanitária da Pecuária Brasileira |
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Cosate, Maria Raquel VenturimSakamoto, TetsuMendes, Tiago Antônio de OliveiraMoreira, Élvio CarlosSilva, Carlos Gustavo Regis daBrasil, Bruno dos Santos Alves FigueiredoOliveira, Camila Stefanie Fonseca deAzevedo, Vasco Ariston de CarvalhoOrtega, José MiguelLeite, Rômulo CerqueiraHaddad, João Paulo2017-07-13T19:18:37Z2017-07-13T19:18:37Z2017COSATE, M. R. V. et al. Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock. BMC Veterinary Research, v. 13, p. 177, 2017.1746-6148https://www.arca.fiocruz.br/handle/icict/2008510.1186/s12917-017-1081-9Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Instituto Nacional de Ciência e Tecnologia (INCT) de Informação Genético-Sanitária da Pecuária BrasileiraUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Biologia Parasitária. Salvador, BA, BrasilEmbrapa Agroenergia. Brasília, DF, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas de Minas Gerais. Departamento de Genética. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, BrasilLeptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serovar Hardjo remain important species associated with this reproductive disease in livestock production. Previous studies on Brazilian livestock have reported that L. interrogans serovar Hardjo is the most prevalent leptospiral agent in this country and is related to clinical signs of leptospirosis, which lead to economic losses in production. Here, we described the isolation of three clinical strains (Norma, Lagoa and Bolivia) obtained from leptospirosis outbreaks that occurred in Minas Gerais state in 1994 and 2008. Results: Serological and molecular typing using housekeeping (secY and 16SrRNA) and rfb locus (ORF22 and ORF36) genes were applied for the identification and comparative analysis of Leptospira spp. Our results identified the three isolates as L. interrogans serogroup Sejroe serovar Hardjo and confirmed the occurrence of this bacterial strain in Brazilian livestock. Genetic analysis using ORF22 and ORF36 grouped the Leptospira into serogroup Sejroe and subtype Hardjoprajitno. Genetic approaches were also applied to compare distinct serovars of L. interrogans strains by verifying the copy numbers of the IS1500 and IS1533 insertion sequences (ISs). The IS1500 copy number varied among the analyzed L. interrogans strains. Conclusion: This study provides evidence that L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjoprajitno causes bovine leptospirosis in Brazilian production. The molecular results suggested that rfb locus (ORF22 and ORF36) could improve epidemiological studies by allowing the identification of Leptospira spp. at the serogroup level. Additionally, the IS1500 and IS1533 IS copy number analysis suggested distinct genomic features among closely related leptospiral strains.engBioMed CentralLeptospira interrogansLeptospiroseGadoAnimaisLeptospira interrogansLeptospirosisLivestockAnimalsMolecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestockinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20085/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCosate MRV Molecular typing....pdfCosate MRV Molecular typing....pdfapplication/pdf815693https://www.arca.fiocruz.br/bitstream/icict/20085/2/Cosate%20MRV%20Molecular%20typing....pdf4116997851dfd9b8efdc5a97f3658e8fMD52TEXTCosate MRV Molecular 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dc.title.pt_BR.fl_str_mv |
Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
title |
Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
spellingShingle |
Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock Cosate, Maria Raquel Venturim Leptospira interrogans Leptospirose Gado Animais Leptospira interrogans Leptospirosis Livestock Animals |
title_short |
Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
title_full |
Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
title_fullStr |
Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
title_full_unstemmed |
Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
title_sort |
Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
author |
Cosate, Maria Raquel Venturim |
author_facet |
Cosate, Maria Raquel Venturim Sakamoto, Tetsu Mendes, Tiago Antônio de Oliveira Moreira, Élvio Carlos Silva, Carlos Gustavo Regis da Brasil, Bruno dos Santos Alves Figueiredo Oliveira, Camila Stefanie Fonseca de Azevedo, Vasco Ariston de Carvalho Ortega, José Miguel Leite, Rômulo Cerqueira Haddad, João Paulo |
author_role |
author |
author2 |
Sakamoto, Tetsu Mendes, Tiago Antônio de Oliveira Moreira, Élvio Carlos Silva, Carlos Gustavo Regis da Brasil, Bruno dos Santos Alves Figueiredo Oliveira, Camila Stefanie Fonseca de Azevedo, Vasco Ariston de Carvalho Ortega, José Miguel Leite, Rômulo Cerqueira Haddad, João Paulo |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cosate, Maria Raquel Venturim Sakamoto, Tetsu Mendes, Tiago Antônio de Oliveira Moreira, Élvio Carlos Silva, Carlos Gustavo Regis da Brasil, Bruno dos Santos Alves Figueiredo Oliveira, Camila Stefanie Fonseca de Azevedo, Vasco Ariston de Carvalho Ortega, José Miguel Leite, Rômulo Cerqueira Haddad, João Paulo |
dc.subject.other.pt_BR.fl_str_mv |
Leptospira interrogans Leptospirose Gado Animais |
topic |
Leptospira interrogans Leptospirose Gado Animais Leptospira interrogans Leptospirosis Livestock Animals |
dc.subject.en.pt_BR.fl_str_mv |
Leptospira interrogans Leptospirosis Livestock Animals |
description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Instituto Nacional de Ciência e Tecnologia (INCT) de Informação Genético-Sanitária da Pecuária Brasileira |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-07-13T19:18:37Z |
dc.date.available.fl_str_mv |
2017-07-13T19:18:37Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSATE, M. R. V. et al. Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock. BMC Veterinary Research, v. 13, p. 177, 2017. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/20085 |
dc.identifier.issn.pt_BR.fl_str_mv |
1746-6148 |
dc.identifier.doi.none.fl_str_mv |
10.1186/s12917-017-1081-9 |
identifier_str_mv |
COSATE, M. R. V. et al. Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock. BMC Veterinary Research, v. 13, p. 177, 2017. 1746-6148 10.1186/s12917-017-1081-9 |
url |
https://www.arca.fiocruz.br/handle/icict/20085 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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BioMed Central |
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BioMed Central |
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