Simvastatin modulates mesenchymal stromal cell proliferation and gene expression

Detalhes bibliográficos
Autor(a) principal: Zanette, Dalila Lucíola
Data de Publicação: 2015
Outros Autores: Lorenzi, Julio Cesar Cetrulo, Panepucci, Rodrigo Alexandre, Palma, Patricia Vianna Bonini, Santos, Daiane Fernanda dos, Prata, Karen Lima, Silva, Wilson Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20518
Resumo: Zanette, Dalila Lucíola “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Zanette, Dalila LucíolaLorenzi, Julio Cesar CetruloPanepucci, Rodrigo AlexandrePalma, Patricia Vianna BoniniSantos, Daiane Fernanda dosPrata, Karen LimaSilva, Wilson Araújo2017-08-04T13:58:55Z2017-08-04T13:58:55Z2015ZANETTE, D. L. et al. Simvastatin modulates mesenchymal stromal cell proliferation and gene expression. PLoS One, v. 10, n. 4, p. e0120137, 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/2051810.1371/journal.pone.0120137Zanette, Dalila Lucíola “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.grants 2009/09443-2 and 2013/08135-2, São Paulo Research Foundation (FAPESP) and CNPqUniversity of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, BrazilCenter for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, BrazilCenter for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, BrazilCenter for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, BrazilStatins are widely used hypocholesterolemic drugs that block the mevalonate pathway, responsible for the biosysnthesis of cholesterol. However, statins also have pleiotropic effects that interfere with several signaling pathways. Mesenchymal stromal cells (MSC) are a heterogeneous mixture of cells that can be isolated from a variety of tissues and are identified by the expression of a panel of surface markers and by their ability to differentiate in vitro into osteocytes, adipocytes and chondrocytes. MSC were isolated from amniotic membranes and bone marrows and characterized based on ISCT (International Society for Cell Therapy) minimal criteria. Simvastatin-treated cells and controls were directly assayed by CFSE (Carboxyfluorescein diacetate succinimidyl ester) staining to assess their cell proliferation and their RNA was used for microarray analyses and quantitative PCR (qPCR). These MSC were also evaluated for their ability to inhibit PBMC (peripheral blood mononuclear cells) proliferation. We show here that simvastatin negatively modulates MSC proliferation in a dose-dependent way and regulates the expression of proliferation-related genes. Importantly, we observed that simvastatin increased the percentage of a subset of smaller MSC, which also were actively proliferating. The association of MSC decreased size with increased pluripotency and the accumulating evidence that statins may prevent cellular senescence led us to hypothesize that simvastatin induces a smaller subpopulation that may have increased ability to maintain the entire pool of MSC and also to protect them from cellular senescence induced by long-term cultures/passages in vitro. These results may be important to better understand the pleiotropic effects of statins and its effects on the biology of cells with regenerative potential.engPublic Library of ScienceSimvastatinaCélulas estromais mesenquimaisGeneticaProliferação celularLeucócitos mononuclearesCélulas cultivadasHumanosSimvastatinMesenchymal Stromal CellsGene ExpressionCell ProliferationLeukocytes, MononuclearCells, CulturedHumansSimvastatin modulates mesenchymal stromal cell proliferation and gene expressioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20518/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALZanette DL Simvastatin modulates....pdfZanette DL Simvastatin modulates....pdfapplication/pdf2603177https://www.arca.fiocruz.br/bitstream/icict/20518/2/Zanette%20DL%20Simvastatin%20modulates....pdf82bfaf4d3ecc0951b7f4623b9e337719MD52TEXTZanette DL Simvastatin modulates....pdf.txtZanette DL Simvastatin modulates....pdf.txtExtracted texttext/plain50966https://www.arca.fiocruz.br/bitstream/icict/20518/3/Zanette%20DL%20Simvastatin%20modulates....pdf.txtada1abef70672237e8aaa9387504c516MD53icict/205182023-03-15 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dc.title.pt_BR.fl_str_mv Simvastatin modulates mesenchymal stromal cell proliferation and gene expression
title Simvastatin modulates mesenchymal stromal cell proliferation and gene expression
spellingShingle Simvastatin modulates mesenchymal stromal cell proliferation and gene expression
Zanette, Dalila Lucíola
Simvastatina
Células estromais mesenquimais
Genetica
Proliferação celular
Leucócitos mononucleares
Células cultivadas
Humanos
Simvastatin
Mesenchymal Stromal Cells
Gene Expression
Cell Proliferation
Leukocytes, Mononuclear
Cells, Cultured
Humans
title_short Simvastatin modulates mesenchymal stromal cell proliferation and gene expression
title_full Simvastatin modulates mesenchymal stromal cell proliferation and gene expression
title_fullStr Simvastatin modulates mesenchymal stromal cell proliferation and gene expression
title_full_unstemmed Simvastatin modulates mesenchymal stromal cell proliferation and gene expression
title_sort Simvastatin modulates mesenchymal stromal cell proliferation and gene expression
author Zanette, Dalila Lucíola
author_facet Zanette, Dalila Lucíola
Lorenzi, Julio Cesar Cetrulo
Panepucci, Rodrigo Alexandre
Palma, Patricia Vianna Bonini
Santos, Daiane Fernanda dos
Prata, Karen Lima
Silva, Wilson Araújo
author_role author
author2 Lorenzi, Julio Cesar Cetrulo
Panepucci, Rodrigo Alexandre
Palma, Patricia Vianna Bonini
Santos, Daiane Fernanda dos
Prata, Karen Lima
Silva, Wilson Araújo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Zanette, Dalila Lucíola
Lorenzi, Julio Cesar Cetrulo
Panepucci, Rodrigo Alexandre
Palma, Patricia Vianna Bonini
Santos, Daiane Fernanda dos
Prata, Karen Lima
Silva, Wilson Araújo
dc.subject.other.pt_BR.fl_str_mv Simvastatina
Células estromais mesenquimais
Genetica
Proliferação celular
Leucócitos mononucleares
Células cultivadas
Humanos
topic Simvastatina
Células estromais mesenquimais
Genetica
Proliferação celular
Leucócitos mononucleares
Células cultivadas
Humanos
Simvastatin
Mesenchymal Stromal Cells
Gene Expression
Cell Proliferation
Leukocytes, Mononuclear
Cells, Cultured
Humans
dc.subject.en.pt_BR.fl_str_mv Simvastatin
Mesenchymal Stromal Cells
Gene Expression
Cell Proliferation
Leukocytes, Mononuclear
Cells, Cultured
Humans
description Zanette, Dalila Lucíola “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2017-08-04T13:58:55Z
dc.date.available.fl_str_mv 2017-08-04T13:58:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ZANETTE, D. L. et al. Simvastatin modulates mesenchymal stromal cell proliferation and gene expression. PLoS One, v. 10, n. 4, p. e0120137, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20518
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0120137
identifier_str_mv ZANETTE, D. L. et al. Simvastatin modulates mesenchymal stromal cell proliferation and gene expression. PLoS One, v. 10, n. 4, p. e0120137, 2015.
1932-6203
10.1371/journal.pone.0120137
url https://www.arca.fiocruz.br/handle/icict/20518
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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