Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles

Detalhes bibliográficos
Autor(a) principal: Santos, Ethel Bayer
Data de Publicação: 2014
Outros Autores: Lima, Fábio Mitsuo, Ruiz, Jeronimo Conceição, Almeida, Igor Correa, Silveira, José Franco da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9398
Resumo: Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil/University of Texas at El Paso. Border Biomedical Research Center. Department of Biological Sciences. El Paso, TX, USA
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spelling Santos, Ethel BayerLima, Fábio MitsuoRuiz, Jeronimo ConceiçãoAlmeida, Igor CorreaSilveira, José Franco da2015-01-28T15:44:27Z2015-01-28T15:44:27Z2014SANTOS, Ethel Bayer et al. Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles. Molecular & Biochemical Parasitology; v. 193, n. 2. p. 71–74, 20140166-6851https://www.arca.fiocruz.br/handle/icict/939810.1016/j.molbiopara.2014.02.004engElsevier LtdaCharacterization of the small RNA content of Trypanosoma cruzi extracellular vesiclesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil/University of Texas at El Paso. Border Biomedical Research Center. Department of Biological Sciences. El Paso, TX, USAUniversidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, BrazilUniversity of Texas at El Paso. Border Biomedical Research Center. Department of Biological Sciences. El Paso, TX, USAUniversidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilA growing body of evidence in mammalian cells indicates that secreted vesicles can be used to mediate intercellular communication processes by transferring various bioactive molecules, including mRNAs and microRNAs. Based on these findings, we decided to analyze whether Trypanosoma cruzi-derived extracellular vesicles contain RNA molecules and performed a deep sequencing and genome-wide analysis of a size-fractioned cDNA library (16–40 nt) from extracellular vesicles secreted by noninfective epimastigote and infective metacyclic trypomastigote forms. Our data show that the small RNAs contained in these extracellular vesicles originate from multiple sources, including tRNAs. In addition, our results reveal that the variety and expression of small RNAs are different between parasite stages, suggesting diverse functions. Taken together, these observations call attention to the potential regulatory functions that these RNAs might play once transferred between parasites and/or to mammalian host cells.Trypanosoma cruziExtracellular vesiclesSmall noncoding RNAHigh-throughput data analysisComparative transcriptomeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9398/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_005.pdf2014_005.pdfapplication/pdf462903https://www.arca.fiocruz.br/bitstream/icict/9398/2/2014_005.pdf08098e0afd7f7f5dc2ed76e7a63f1c78MD52TEXT2014_005.pdf.txt2014_005.pdf.txtExtracted texttext/plain25627https://www.arca.fiocruz.br/bitstream/icict/9398/3/2014_005.pdf.txtac69d07e9de143842daaf4ed3d910a05MD53icict/93982019-06-19 10:06:35.277oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:06:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
title Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
spellingShingle Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
Santos, Ethel Bayer
Trypanosoma cruzi
Extracellular vesicles
Small noncoding RNA
High-throughput data analysis
Comparative transcriptome
title_short Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
title_full Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
title_fullStr Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
title_full_unstemmed Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
title_sort Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
author Santos, Ethel Bayer
author_facet Santos, Ethel Bayer
Lima, Fábio Mitsuo
Ruiz, Jeronimo Conceição
Almeida, Igor Correa
Silveira, José Franco da
author_role author
author2 Lima, Fábio Mitsuo
Ruiz, Jeronimo Conceição
Almeida, Igor Correa
Silveira, José Franco da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santos, Ethel Bayer
Lima, Fábio Mitsuo
Ruiz, Jeronimo Conceição
Almeida, Igor Correa
Silveira, José Franco da
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Extracellular vesicles
Small noncoding RNA
High-throughput data analysis
Comparative transcriptome
topic Trypanosoma cruzi
Extracellular vesicles
Small noncoding RNA
High-throughput data analysis
Comparative transcriptome
description Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil/University of Texas at El Paso. Border Biomedical Research Center. Department of Biological Sciences. El Paso, TX, USA
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-01-28T15:44:27Z
dc.date.available.fl_str_mv 2015-01-28T15:44:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Ethel Bayer et al. Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles. Molecular & Biochemical Parasitology; v. 193, n. 2. p. 71–74, 2014
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9398
dc.identifier.issn.none.fl_str_mv 0166-6851
dc.identifier.doi.none.fl_str_mv 10.1016/j.molbiopara.2014.02.004
identifier_str_mv SANTOS, Ethel Bayer et al. Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles. Molecular & Biochemical Parasitology; v. 193, n. 2. p. 71–74, 2014
0166-6851
10.1016/j.molbiopara.2014.02.004
url https://www.arca.fiocruz.br/handle/icict/9398
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Ltda
publisher.none.fl_str_mv Elsevier Ltda
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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