Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/4190 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. |
id |
CRUZ_3c1d666f1e9cca697547f8d92ab35166 |
---|---|
oai_identifier_str |
oai:www.arca.fiocruz.br:icict/4190 |
network_acronym_str |
CRUZ |
network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
repository_id_str |
2135 |
spelling |
Soares, Milena Botelho PereiraBellintani, Moema CortizoRibeiro, Ivone MariaTomassini, Therezinha Coelho BarbosaSantos, Ricardo Ribeiro dos2012-07-18T18:18:01Z2012-07-18T18:18:01Z2003SOARES, M .B. et al. Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. European Journal of Pharmacology, v. 459, n. 1, p. 107-112, jan. 20030014-2999S0014-2999(02)02829-7https://www.arca.fiocruz.br/handle/icict/4190engInhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2003Centro de Pesquisas Gonçalo MonizFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo MonizSalvadorFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Physalis angulata L. is an annual herb widely used in popular medicine for the treatment of a variety of pathologies. Here, we tested immunomodulatory activities of physalins, seco-steroids purified from P. angulata extracts. Addition of physalins B, F or G, but not D, caused a reduction in nitric oxide production by macrophages stimulated with lipopolysaccaride and interferon-g. In the presence of physalin B, macrophages stimulated with lipopolysaccaride, alone or in combination with interferon-g, produced lower levels of tumour necrosis factor (TNF)-a, interleukin-6 and interleukin-12. The inhibitory activity of physalin B, unlike that of dexamethasone, was not reversed by RU486 [(4-dimethylamino) phenyl-17h-hydroxy-17-(1-propynyl)estra-4,9-dien-3-one], an antiglucocorticoid. Physalin B-treated mice had lower levels of serum TNF-a than control mice after lipopolysaccaride challenge. More importantly, mice injected with physalins B, F or G survived after a lethal lipopolysaccaride challenge. These results demonstrate that seco-steroids from P. angulata are potent immunomodulatory substances and act through a mechanism distinct from that of dexamethasone.SteroidImmunomodulation(Physalis angulata L.)Endotoxic shockLipopolissacarídeos/farmacologiaAtivação de Macrófagos/efeitos de drogasPhysalis/químicaFitosteróis/farmacologiaSobrevivência Celular/efeitos de drogasCélulas CultivadasCitocinas/sangueCitocinas/metabolismoRelação Dose-Resposta a DrogaAntagonistas de Hormônios/farmacologiaLactonas/farmacologiaMacrófagos Peritoneais/citologiaCamundongos Endogâmicos BALB CMifepristona/farmacologiaOxido Nítrico/sangueExtratos Vegetais/químicainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSoares MB Inhibition of macrophage activation....pdfSoares MB Inhibition of macrophage activation....pdfapplication/pdf194748https://www.arca.fiocruz.br/bitstream/icict/4190/1/Soares%20MB%20Inhibition%20of%20macrophage%20activation....pdf42b363a4b5df1780f7b7e551ff9992e6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81990https://www.arca.fiocruz.br/bitstream/icict/4190/2/license.txtb6ead4607e393dee1190c21ba08397beMD52TEXTSoares MB Inhibition of macrophage activation....pdf.txtSoares MB Inhibition of macrophage activation....pdf.txtExtracted texttext/plain25878https://www.arca.fiocruz.br/bitstream/icict/4190/5/Soares%20MB%20Inhibition%20of%20macrophage%20activation....pdf.txt759ae2b03d483b345d99a5670d74c7d6MD55THUMBNAILSoares MB Inhibition of macrophage activation....pdf.jpgSoares MB Inhibition of macrophage activation....pdf.jpgGenerated Thumbnailimage/jpeg2291https://www.arca.fiocruz.br/bitstream/icict/4190/4/Soares%20MB%20Inhibition%20of%20macrophage%20activation....pdf.jpg1c71651d4321b266c787fff168a54784MD54icict/41902023-03-15 14:32:57.203oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. |
dc.title.original.pt_BR.fl_str_mv |
Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. |
title |
Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. |
spellingShingle |
Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. Soares, Milena Botelho Pereira Steroid Immunomodulation (Physalis angulata L.) Endotoxic shock Lipopolissacarídeos/farmacologia Ativação de Macrófagos/efeitos de drogas Physalis/química Fitosteróis/farmacologia Sobrevivência Celular/efeitos de drogas Células Cultivadas Citocinas/sangue Citocinas/metabolismo Relação Dose-Resposta a Droga Antagonistas de Hormônios/farmacologia Lactonas/farmacologia Macrófagos Peritoneais/citologia Camundongos Endogâmicos BALB C Mifepristona/farmacologia Oxido Nítrico/sangue Extratos Vegetais/química |
title_short |
Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. |
title_full |
Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. |
title_fullStr |
Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. |
title_full_unstemmed |
Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. |
title_sort |
Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. |
author |
Soares, Milena Botelho Pereira |
author_facet |
Soares, Milena Botelho Pereira Bellintani, Moema Cortizo Ribeiro, Ivone Maria Tomassini, Therezinha Coelho Barbosa Santos, Ricardo Ribeiro dos |
author_role |
author |
author2 |
Bellintani, Moema Cortizo Ribeiro, Ivone Maria Tomassini, Therezinha Coelho Barbosa Santos, Ricardo Ribeiro dos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Soares, Milena Botelho Pereira Bellintani, Moema Cortizo Ribeiro, Ivone Maria Tomassini, Therezinha Coelho Barbosa Santos, Ricardo Ribeiro dos |
dc.subject.en.pt_BR.fl_str_mv |
Steroid Immunomodulation (Physalis angulata L.) Endotoxic shock |
topic |
Steroid Immunomodulation (Physalis angulata L.) Endotoxic shock Lipopolissacarídeos/farmacologia Ativação de Macrófagos/efeitos de drogas Physalis/química Fitosteróis/farmacologia Sobrevivência Celular/efeitos de drogas Células Cultivadas Citocinas/sangue Citocinas/metabolismo Relação Dose-Resposta a Droga Antagonistas de Hormônios/farmacologia Lactonas/farmacologia Macrófagos Peritoneais/citologia Camundongos Endogâmicos BALB C Mifepristona/farmacologia Oxido Nítrico/sangue Extratos Vegetais/química |
dc.subject.decs.pt_BR.fl_str_mv |
Lipopolissacarídeos/farmacologia Ativação de Macrófagos/efeitos de drogas Physalis/química Fitosteróis/farmacologia Sobrevivência Celular/efeitos de drogas Células Cultivadas Citocinas/sangue Citocinas/metabolismo Relação Dose-Resposta a Droga Antagonistas de Hormônios/farmacologia Lactonas/farmacologia Macrófagos Peritoneais/citologia Camundongos Endogâmicos BALB C Mifepristona/farmacologia Oxido Nítrico/sangue Extratos Vegetais/química |
description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003 |
dc.date.accessioned.fl_str_mv |
2012-07-18T18:18:01Z |
dc.date.available.fl_str_mv |
2012-07-18T18:18:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOARES, M .B. et al. Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. European Journal of Pharmacology, v. 459, n. 1, p. 107-112, jan. 2003 |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/4190 |
dc.identifier.issn.none.fl_str_mv |
0014-2999 |
dc.identifier.other.none.fl_str_mv |
S0014-2999(02)02829-7 |
identifier_str_mv |
SOARES, M .B. et al. Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. European Journal of Pharmacology, v. 459, n. 1, p. 107-112, jan. 2003 0014-2999 S0014-2999(02)02829-7 |
url |
https://www.arca.fiocruz.br/handle/icict/4190 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
collection |
Repositório Institucional da FIOCRUZ (ARCA) |
bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/4190/1/Soares%20MB%20Inhibition%20of%20macrophage%20activation....pdf https://www.arca.fiocruz.br/bitstream/icict/4190/2/license.txt https://www.arca.fiocruz.br/bitstream/icict/4190/5/Soares%20MB%20Inhibition%20of%20macrophage%20activation....pdf.txt https://www.arca.fiocruz.br/bitstream/icict/4190/4/Soares%20MB%20Inhibition%20of%20macrophage%20activation....pdf.jpg |
bitstream.checksum.fl_str_mv |
42b363a4b5df1780f7b7e551ff9992e6 b6ead4607e393dee1190c21ba08397be 759ae2b03d483b345d99a5670d74c7d6 1c71651d4321b266c787fff168a54784 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
_version_ |
1798324950079111168 |