Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells

Detalhes bibliográficos
Autor(a) principal: Cestari, Igor
Data de Publicação: 2010
Outros Autores: Ramirez, Marcel I
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20980
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil.
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spelling Cestari, IgorRamirez, Marcel I2017-09-12T17:40:57Z2017-09-12T17:40:57Z2010CESTARI, Igor; RAMIREZ, Marcel I. Inefficient Complement System Clearance of Trypanosoma cruzi Metacyclic Trypomastigotes Enables Resistant Strains to Invade Eukaryotic Cells. Plos One, v.5, n.3, e9721, 11p, 2010.1932-6203https://www.arca.fiocruz.br/handle/icict/2098010.1371/journal.pone.0009721engPublic Library of ScienceTrypanosoma cruziCélulas EucarióticasInfecçãoDoença de ChagasTrypanosoma cruziEukaryotic CellsT. cruzi strainscomplement systemT. cruzi infectionChagas diseaseInefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil.The complement system is the main arm of the vertebrate innate immune system against pathogen infection. For the protozoan Trypanosoma cruzi, the causative agent of Chagas disease, subverting the complement system and invading the host cells is crucial to succeed in infection. However, little attention has focused on whether the complement system can effectively control T. cruzi infection. To address this question, we decided to analyse: 1) which complement pathways are activated by T. cruzi using strains isolated from different hosts, 2) the capacity of these strains to resist the complement-mediated killing at nearly physiological conditions, and 3) whether the complement system could limit or control T. cruzi invasion of eukaryotic cells. The complement activating molecules C1q, C3, mannan-binding lectin and ficolins bound to all strains analysed; however, C3b and C4b deposition assays revealed that T. cruzi activates mainly the lectin and alternative complement pathways in non-immune human serum. Strikingly, we detected that metacyclic trypomastigotes of some T. cruzi strains were highly susceptible to complement-mediated killing in non-immune serum, while other strains were resistant. Furthermore, the rate of parasite invasion in eukaryotic cells was decreased by non-immune serum. Altogether, these results establish that the complement system recognizes T. cruzi metacyclic trypomastigotes, resulting in killing of susceptible strains. The complement system, therefore, acts as a physiological barrier which resistant strains have to evade for successful host infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20980/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALigor_cestari_etal_IOC_2010.pdfigor_cestari_etal_IOC_2010.pdfapplication/pdf753593https://www.arca.fiocruz.br/bitstream/icict/20980/2/igor_cestari_etal_IOC_2010.pdfccd763efd1a75665ddfb73a4a3d16964MD52TEXTigor_cestari_etal_IOC_2010.pdf.txtigor_cestari_etal_IOC_2010.pdf.txtExtracted texttext/plain55174https://www.arca.fiocruz.br/bitstream/icict/20980/3/igor_cestari_etal_IOC_2010.pdf.txtd4f532d3a8a2a0d84a05e63fcb753e44MD53icict/209802018-08-15 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dc.title.pt_BR.fl_str_mv Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells
title Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells
spellingShingle Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells
Cestari, Igor
Trypanosoma cruzi
Células Eucarióticas
Infecção
Doença de Chagas
Trypanosoma cruzi
Eukaryotic Cells
T. cruzi strains
complement system
T. cruzi infection
Chagas disease
title_short Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells
title_full Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells
title_fullStr Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells
title_full_unstemmed Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells
title_sort Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells
author Cestari, Igor
author_facet Cestari, Igor
Ramirez, Marcel I
author_role author
author2 Ramirez, Marcel I
author2_role author
dc.contributor.author.fl_str_mv Cestari, Igor
Ramirez, Marcel I
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Células Eucarióticas
Infecção
Doença de Chagas
topic Trypanosoma cruzi
Células Eucarióticas
Infecção
Doença de Chagas
Trypanosoma cruzi
Eukaryotic Cells
T. cruzi strains
complement system
T. cruzi infection
Chagas disease
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Eukaryotic Cells
T. cruzi strains
complement system
T. cruzi infection
Chagas disease
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2017-09-12T17:40:57Z
dc.date.available.fl_str_mv 2017-09-12T17:40:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CESTARI, Igor; RAMIREZ, Marcel I. Inefficient Complement System Clearance of Trypanosoma cruzi Metacyclic Trypomastigotes Enables Resistant Strains to Invade Eukaryotic Cells. Plos One, v.5, n.3, e9721, 11p, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20980
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0009721
identifier_str_mv CESTARI, Igor; RAMIREZ, Marcel I. Inefficient Complement System Clearance of Trypanosoma cruzi Metacyclic Trypomastigotes Enables Resistant Strains to Invade Eukaryotic Cells. Plos One, v.5, n.3, e9721, 11p, 2010.
1932-6203
10.1371/journal.pone.0009721
url https://www.arca.fiocruz.br/handle/icict/20980
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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