A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11679 |
Resumo: | La Jolla Bioengineering Institute. Center for Malaria Research. San Diego, California, USA. |
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Ong, Peng KaiMeays, DianaFrangos, John A.Carvalho, Leonardo2015-09-21T17:25:11Z2015-09-21T17:25:11Z2013ONG, Peng Kai; et al. A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria. Microcirculation, v.20, p. 394–404, 2013.1549-8719https://www.arca.fiocruz.br/handle/icict/1167910.1111/micc.12034engWiley Online LibraryCrânioCerebroarteriolar responsesCranial window superfusionEndothelial nitric oxide synthaseExperimental cerebral malariaPlasmodium berghei ANKAPlasmodium bergheiMaláriaVeias CerebraisMurinaA Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malariainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLa Jolla Bioengineering Institute. Center for Malaria Research. San Diego, California, USA.La Jolla Bioengineering Institute. Center for Malaria Research. San Diego, California, USA.La Jolla Bioengineering Institute. Center for Malaria Research. San Diego, California, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Objective: The acute implantation of a cranial window for studying cerebroarteriolar reactivity in living animals involves a highly surgically invasive craniotomy procedure at the time of experimentation, which limits its application in severely ill animals such as in the experimental murine model of cerebral malaria (ECM). To overcome this problem, a chronic window implantation scheme was designed and implemented. Methods: A partial craniotomy is first performed by creating a skull bone flap in the healthy mice, which are then left to recover for one to two weeks, followed by infection to induce ECM. Uninfected animals are utilized as control. When cranial superfusion is needed, the bone flap is retracted and window implantation completed by assembling a perfusion chamber for compound delivery to the exposed brain surface. The presurgical step is intended to minimize surgical trauma on the day of experimentation. Results: Chronic preparations in uninfected mice exhibited remarkably improved stability over acute ones by significantly reducing periarteriolar tissue damage and enhancing cerebroarteriolar dilator responses. The chronic scheme was successfully implemented in ECM mice, which unveiled novel preliminary insights into impaired cerebroarteriolar reactivity and eNOS dysfunction. Conclusion: The chronic scheme presents an innovative approach for advancing our mechanistic understanding on cerebrovascular dysfunction in ECM.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11679/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALleonardo_carvalho_etal_IOC_2013.pdfapplication/pdf542422https://www.arca.fiocruz.br/bitstream/icict/11679/2/leonardo_carvalho_etal_IOC_2013.pdff7c72603e9ddc2ca14284cf9fd704bf0MD52TEXTleonardo_carvalho_etal_IOC_2013.pdf.txtleonardo_carvalho_etal_IOC_2013.pdf.txtExtracted texttext/plain53841https://www.arca.fiocruz.br/bitstream/icict/11679/3/leonardo_carvalho_etal_IOC_2013.pdf.txt58dc161c845dd1eac5d51a48b493ccebMD53icict/116792022-06-24 13:08:57.109oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:08:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria |
| title |
A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria |
| spellingShingle |
A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria Ong, Peng Kai Crânio Cerebroarteriolar responses Cranial window superfusion Endothelial nitric oxide synthase Experimental cerebral malaria Plasmodium berghei ANKA Plasmodium berghei Malária Veias Cerebrais Murina |
| title_short |
A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria |
| title_full |
A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria |
| title_fullStr |
A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria |
| title_full_unstemmed |
A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria |
| title_sort |
A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria |
| author |
Ong, Peng Kai |
| author_facet |
Ong, Peng Kai Meays, Diana Frangos, John A. Carvalho, Leonardo |
| author_role |
author |
| author2 |
Meays, Diana Frangos, John A. Carvalho, Leonardo |
| author2_role |
author author author |
| dc.contributor.author.fl_str_mv |
Ong, Peng Kai Meays, Diana Frangos, John A. Carvalho, Leonardo |
| dc.subject.other.pt_BR.fl_str_mv |
Crânio |
| topic |
Crânio Cerebroarteriolar responses Cranial window superfusion Endothelial nitric oxide synthase Experimental cerebral malaria Plasmodium berghei ANKA Plasmodium berghei Malária Veias Cerebrais Murina |
| dc.subject.en.pt_BR.fl_str_mv |
Cerebroarteriolar responses Cranial window superfusion Endothelial nitric oxide synthase Experimental cerebral malaria Plasmodium berghei ANKA |
| dc.subject.decs.pt_BR.fl_str_mv |
Plasmodium berghei Malária Veias Cerebrais Murina |
| description |
La Jolla Bioengineering Institute. Center for Malaria Research. San Diego, California, USA. |
| publishDate |
2013 |
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2013 |
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2015-09-21T17:25:11Z |
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2015-09-21T17:25:11Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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ONG, Peng Kai; et al. A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria. Microcirculation, v.20, p. 394–404, 2013. |
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https://www.arca.fiocruz.br/handle/icict/11679 |
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1549-8719 |
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10.1111/micc.12034 |
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ONG, Peng Kai; et al. A Chronic Scheme of Cranial Window Preparation to Study Pial Vascular Reactivity in Murine Cerebral Malaria. Microcirculation, v.20, p. 394–404, 2013. 1549-8719 10.1111/micc.12034 |
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Wiley Online Library |
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