Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage

Detalhes bibliográficos
Autor(a) principal: Hojo-Souza, Natália Satchiko
Data de Publicação: 2020
Outros Autores: Azevedo, Patrick Orestes de, Castro, Júlia Teixeira de, Carvalho, Andréa Teixeira de, Lieberman, Judy, Giusta, Caroline Junqueira, Gazzinelli, Ricardo Tostes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43898
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil
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spelling Hojo-Souza, Natália SatchikoAzevedo, Patrick Orestes deCastro, Júlia Teixeira deCarvalho, Andréa Teixeira deLieberman, JudyGiusta, Caroline JunqueiraGazzinelli, Ricardo Tostes2020-10-09T13:34:05Z2020-10-09T13:34:05Z2020HOJO-SOUZA, Natalia Satchiko et al. Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage. PLoS Pathog, v. 16, n. 9, e1008840, 2020. https://doi.org/10.1371/journal.ppat.10088401553-7366https://www.arca.fiocruz.br/handle/icict/4389810.1371/journal.ppat.1008840engPublic Library of ScienceContributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stageinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilProgram in Cellular and Molecular Medicine. Boston Children’s Hospital and Department of Pediatrics, Harvard Medical School. Boston, MA, United States of AmericaFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/Program in Cellular and Molecular Medicine. Boston Children’s Hospital and Department of Pediatrics, Harvard Medical School. Boston, MA, United States of AmericaFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/Division of Infectious Disease and Immunology. University of Massachusetts Medical School.Worcester, MA, United States of America/Fundação Oswaldo Cruz. Plataforma de Medicina Translacional. Ribeirão Preto, SP, BrazilP. vivax-infected Retics (iRetics) express human leukocyte antigen class I (HLA-I), are recognized by CD8+ T cells and killed by granulysin (GNLY) and granzymes. However, how Plasmodium infection induces MHC-I expression on Retics is unknown. In addition, whether GNLY helps control Plasmodium infection in vivo has not been studied. Here, we examine these questions using rodent infection with the P. yoelii 17XNL strain, which has tropism for Retics. Infection with P. yoelii caused extramedullary erythropoiesis, reticulocytosis and expansion of CD8+CD44+CD62L- IFN-γ-producing T cells that form immune synapses with iRetics. We now provide evidence that MHC-I expression by iRetic is dependent on IFN-γ-induced transcription of IRF-1, MHC-I and β2-microglobulin (β2-m) in erythroblasts. Consistently, CTLs from infected wild type (WT) mice formed immune synapses with iRetics in an IFN-γ- and MHC-I-dependent manner. When challenged with P. yoelii 17XNL, WT mice cleared parasitemia and survived, while IFN-γ KO mice remained parasitemic and all died. β2-m KO mice that do not express MHC-I and have virtually no CD8+ T cells had prolonged parasitemia, and 80% survived. Because mice do not express GNLY, GNLY-transgenic mice can be used to assess the in vivo importance of GNLY. Parasite clearance was accelerated in GNLY-transgenic mice and depletion of CD8+ T cells ablated the GNLY-mediated resistance to P. yoelii. Altogether, our results indicate that in addition to previously described mechanisms, IFN-γ promotes host resistance to the Retic-tropic P. yoelii 17XNL strain by promoting MHC-I expression on iRetics that become targets for CD8+ cytotoxic T lymphocytes and GNLY.T cellsCytotoxic T cellsPlasmodium yoeliiErythroblastsParasitic diseasesParasitemiaSpleenBloodinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/43898/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALContributions of IFN-γ and granulysin.pdfContributions of IFN-γ and granulysin.pdfapplication/pdf5199631https://www.arca.fiocruz.br/bitstream/icict/43898/2/Contributions%20of%20IFN-%ce%b3%20and%20granulysin.pdf3ca985272235a27d092713a43c11dfecMD52TEXTContributions of IFN-γ and granulysin.pdf.txtContributions of IFN-γ and 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dc.title.pt_BR.fl_str_mv Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage
title Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage
spellingShingle Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage
Hojo-Souza, Natália Satchiko
T cells
Cytotoxic T cells
Plasmodium yoelii
Erythroblasts
Parasitic diseases
Parasitemia
Spleen
Blood
title_short Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage
title_full Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage
title_fullStr Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage
title_full_unstemmed Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage
title_sort Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage
author Hojo-Souza, Natália Satchiko
author_facet Hojo-Souza, Natália Satchiko
Azevedo, Patrick Orestes de
Castro, Júlia Teixeira de
Carvalho, Andréa Teixeira de
Lieberman, Judy
Giusta, Caroline Junqueira
Gazzinelli, Ricardo Tostes
author_role author
author2 Azevedo, Patrick Orestes de
Castro, Júlia Teixeira de
Carvalho, Andréa Teixeira de
Lieberman, Judy
Giusta, Caroline Junqueira
Gazzinelli, Ricardo Tostes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Hojo-Souza, Natália Satchiko
Azevedo, Patrick Orestes de
Castro, Júlia Teixeira de
Carvalho, Andréa Teixeira de
Lieberman, Judy
Giusta, Caroline Junqueira
Gazzinelli, Ricardo Tostes
dc.subject.en.pt_BR.fl_str_mv T cells
Cytotoxic T cells
Plasmodium yoelii
Erythroblasts
Parasitic diseases
Parasitemia
Spleen
Blood
topic T cells
Cytotoxic T cells
Plasmodium yoelii
Erythroblasts
Parasitic diseases
Parasitemia
Spleen
Blood
description Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-09T13:34:05Z
dc.date.available.fl_str_mv 2020-10-09T13:34:05Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HOJO-SOUZA, Natalia Satchiko et al. Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage. PLoS Pathog, v. 16, n. 9, e1008840, 2020. https://doi.org/10.1371/journal.ppat.1008840
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43898
dc.identifier.issn.pt_BR.fl_str_mv 1553-7366
dc.identifier.doi.none.fl_str_mv 10.1371/journal.ppat.1008840
identifier_str_mv HOJO-SOUZA, Natalia Satchiko et al. Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage. PLoS Pathog, v. 16, n. 9, e1008840, 2020. https://doi.org/10.1371/journal.ppat.1008840
1553-7366
10.1371/journal.ppat.1008840
url https://www.arca.fiocruz.br/handle/icict/43898
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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