α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/51455 |
Resumo: | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil |
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Cunha, Gisele Macêdo Rodrigues daNogueira, Denise SilvaChunga, Juan Atilio JimenezGalvão, Lucia Maria da CunhaChiari, EglerBrito, Carlos Ramon NascimentoSoares, Rodrigo Pedro PintoNogueira, Paula MonalisaFujiwara, Ricardo ToshioHincapie, RobertChaves, Carlos-SanhuezaOliveira, Fabricio Marcus SilvaMarques, Alexandre Ferreira2022-02-24T12:48:57Z2022-02-24T12:48:57Z2021CUNHA, Gisele Macêdo Rodrigues da et al. α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model. PLoS Negl Trop Dis., v. 15, n. 7, e0009613, 2021. doi: 10.1371/journal.pntd.00096131935-2735https://www.arca.fiocruz.br/handle/icict/5145510.1371/journal.pntd.0009613engPublic Library of Scienceα-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidad San Martin de Porres. Lima, PeruUniversidad Nacional Mayor de San Marcos. Faculdad de Ciencias Biologicas. Escuela Profesional de Microbiología y Parasitología. Laboratorio de Parasitología en Fauna Silvestre y Zoonosis. Lima, PeruUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Departamento de Análises Clínicas e Toxicológicas. Natal, RN, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilSchool of Chemistry and Biochemistry. School of Biological Sciences. Georgia Institute of Technology. Atlanta, GA, United States of AmericaSchool of Chemistry and Biochemistry. School of Biological Sciences. Georgia Institute of Technology. Atlanta, GA, United States of AmericaSchool of Chemistry and Biochemistry. School of Biological Sciences. Georgia Institute of Technology. Atlanta, GA, United States of AmericaUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilChagas disease, caused by the protozoan parasite Trypanosoma cruzi, is a significant endemic infectious disease in Latin America and is spreading in the U.S. and Europe with the presence of its insect transmission vector. No approved vaccine against Chagas disease exists. We describe a vaccine candidate based on a carbohydrate found on the T. cruzi cell surface, linked in the vaccine to a virus-like particle that provides a strong and focused immune response. Mice were immunized and challenged with the Trypanosoma cruzi parasites from two strains (Y and Colombian). Vaccination conferred substantial protection of mice against infection, compared with the unvaccinated group. Vaccinated animals presented low parasitemia, increased production of pro-inflammatory cytokines IL-12 and IFN-gamma, decreased cardiomyocyte damage, and rapid clearance of parasite nests from heart tissue. These effects were especially significant at time points modeling chronic disease, an important consideration for this pathogen. We, therefore, believe this is a valuable path to pursue in the development of vaccines against Chagas disease. Chagas disease, caused by the parasite Trypanosoma cruzi, is considered endemic in more than 20 countries but lacks both an approved vaccine and limited treatment for its chronic stage. Chronic infection is most harmful to human health because of long-term parasitic infection of the heart. Here we show that immunization with a virus-like particle vaccine displaying a high density of the immunogenic alpha-Gal trisaccharide (Q beta-alpha Gal) induced several beneficial effects concerning acute and chronic T. cruzi infection in alpha 1,3-galactosyltransferase knockout mice. Approximately 60% of these animals were protected from initial infection with high parasite loads. Vaccinated animals also produced high anti-alpha Gal IgG antibody titers, improved IFN-gamma and IL-12 cytokine production, and controlled parasitemia in the acute phase at 8 days post-infection (dpi) for the Y strain and 22 dpi for the Colombian strain. In the chronic stage of infection (36 and 190 dpi, respectively), all of the vaccinated group survived, showing significantly decreased heart inflammation and clearance of amastigote nests from the heart tissue.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51455/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALα-Gal immunization positively impacts.pdfα-Gal immunization positively impacts.pdfapplication/pdf6451343https://www.arca.fiocruz.br/bitstream/icict/51455/2/%ce%b1-Gal%20immunization%20positively%20impacts.pdf436e12e6396d8b7a385e32d99fa90fecMD52icict/514552022-02-24 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| dc.title.pt_BR.fl_str_mv |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model |
| title |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model |
| spellingShingle |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model Cunha, Gisele Macêdo Rodrigues da |
| title_short |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model |
| title_full |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model |
| title_fullStr |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model |
| title_full_unstemmed |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model |
| title_sort |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model |
| author |
Cunha, Gisele Macêdo Rodrigues da |
| author_facet |
Cunha, Gisele Macêdo Rodrigues da Nogueira, Denise Silva Chunga, Juan Atilio Jimenez Galvão, Lucia Maria da Cunha Chiari, Egler Brito, Carlos Ramon Nascimento Soares, Rodrigo Pedro Pinto Nogueira, Paula Monalisa Fujiwara, Ricardo Toshio Hincapie, Robert Chaves, Carlos-Sanhueza Oliveira, Fabricio Marcus Silva Marques, Alexandre Ferreira |
| author_role |
author |
| author2 |
Nogueira, Denise Silva Chunga, Juan Atilio Jimenez Galvão, Lucia Maria da Cunha Chiari, Egler Brito, Carlos Ramon Nascimento Soares, Rodrigo Pedro Pinto Nogueira, Paula Monalisa Fujiwara, Ricardo Toshio Hincapie, Robert Chaves, Carlos-Sanhueza Oliveira, Fabricio Marcus Silva Marques, Alexandre Ferreira |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cunha, Gisele Macêdo Rodrigues da Nogueira, Denise Silva Chunga, Juan Atilio Jimenez Galvão, Lucia Maria da Cunha Chiari, Egler Brito, Carlos Ramon Nascimento Soares, Rodrigo Pedro Pinto Nogueira, Paula Monalisa Fujiwara, Ricardo Toshio Hincapie, Robert Chaves, Carlos-Sanhueza Oliveira, Fabricio Marcus Silva Marques, Alexandre Ferreira |
| description |
Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil |
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2021 |
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2021 |
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2022-02-24T12:48:57Z |
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2022-02-24T12:48:57Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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CUNHA, Gisele Macêdo Rodrigues da et al. α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model. PLoS Negl Trop Dis., v. 15, n. 7, e0009613, 2021. doi: 10.1371/journal.pntd.0009613 |
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https://www.arca.fiocruz.br/handle/icict/51455 |
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1935-2735 |
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10.1371/journal.pntd.0009613 |
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CUNHA, Gisele Macêdo Rodrigues da et al. α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model. PLoS Negl Trop Dis., v. 15, n. 7, e0009613, 2021. doi: 10.1371/journal.pntd.0009613 1935-2735 10.1371/journal.pntd.0009613 |
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