SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/51559 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. |
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Vieira, Debora Ferreira BarretoSilva, Marcos Alexandre Nunes daAlmeida, Ana Luisa Teixeira deRasinhas, Arthur da CostaMonteiro, Maria EduardaMiranda, Milene DiasMotta, Fernando CoutoSiqueira, Marilda Agudo Mendonça Teixeira deDias, Wendell GirardArchanjo, Bráulio SoaresBozza, Patrícia T.Souza, Thiago Moreno L.Dias, Suelen Silva GomesSoares, Vinicius CardosoBarth, Ortrud Monika2022-03-04T19:19:38Z2022-03-04T19:19:38Z2022VIEIRA, Debora ferreira Barreto et al. SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System. Viruses, v. 14, 201, p. 1 - 21, Jan. 2022.1999-4915https://www.arca.fiocruz.br/handle/icict/5155910.3390/v14020201engMDPISARS-CoV-2.Células Vero-E6Estudos ultraestruturaisMorfogêneseMicroscopia eletrônica de transmissãoMicroscopia eletrônica de varreduraModelagem de microscopia eletrônica 3DSARS-CoV-2.Vero-E6 cellsUltrastructural studiesMorphogenesisTransmission electron microscopyScanning electron microscopy3D electron microscopy modelingSARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro Systeminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Microscopia Eletrônica Rudolph Barth. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Núcleo de Laboratórios de Microscopia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia em Inovação em Doenças da População Negligenciadas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Programa de Imunologia e Inflamação. Rio de Janeiro, RJ, Brasil..Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has impacted public health and the world economy and fueled a worldwide race to approve therapeutic and prophylactic agents, but so far there are no specific antiviral drugs. Understanding the biology of the virus is the first step in structuring strategies to combat it, and in this context several studies have been conducted with the aim of understanding the replication mechanism of SARS-CoV-2 in vitro systems. In this work, studies using transmission and scanning electron microscopy and 3D electron microscopy modeling were performed with the goal of characterizing the morphogenesis of SARS-CoV-2 in Vero-E6 cells. Several ultrastructural changes were observed—such as syncytia formation, cytoplasmic membrane projections, lipid droplets accumulation, proliferation of doublemembrane vesicles derived from the rough endoplasmic reticulum, and alteration of mitochondria. The entry of the virus into cells occurred through endocytosis. Viral particles were observed attached to the cell membrane and in various cellular compartments, and extrusion of viral progeny took place by exocytosis. These findings allow us to infer that Vero-E6 cells are highly susceptible to SARS-CoV-2 infection as described in the literature and their replication cycle is similar to that described with SARS-CoV and MERS-CoV in vitro models.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/51559/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDeboraVieira_OrtudBarth_etal_IOC_2022.pdfDeboraVieira_OrtudBarth_etal_IOC_2022.pdfapplication/pdf17144803https://www.arca.fiocruz.br/bitstream/icict/51559/2/DeboraVieira_OrtudBarth_etal_IOC_2022.pdf2eafd769c9aadc35aebfc8ca0dabd4daMD52icict/515592023-09-05 10:26:21.239oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:26:21Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.pt_BR.fl_str_mv |
SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System |
| title |
SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System |
| spellingShingle |
SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System Vieira, Debora Ferreira Barreto SARS-CoV-2. Células Vero-E6 Estudos ultraestruturais Morfogênese Microscopia eletrônica de transmissão Microscopia eletrônica de varredura Modelagem de microscopia eletrônica 3D SARS-CoV-2. Vero-E6 cells Ultrastructural studies Morphogenesis Transmission electron microscopy Scanning electron microscopy 3D electron microscopy modeling |
| title_short |
SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System |
| title_full |
SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System |
| title_fullStr |
SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System |
| title_full_unstemmed |
SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System |
| title_sort |
SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System |
| author |
Vieira, Debora Ferreira Barreto |
| author_facet |
Vieira, Debora Ferreira Barreto Silva, Marcos Alexandre Nunes da Almeida, Ana Luisa Teixeira de Rasinhas, Arthur da Costa Monteiro, Maria Eduarda Miranda, Milene Dias Motta, Fernando Couto Siqueira, Marilda Agudo Mendonça Teixeira de Dias, Wendell Girard Archanjo, Bráulio Soares Bozza, Patrícia T. Souza, Thiago Moreno L. Dias, Suelen Silva Gomes Soares, Vinicius Cardoso Barth, Ortrud Monika |
| author_role |
author |
| author2 |
Silva, Marcos Alexandre Nunes da Almeida, Ana Luisa Teixeira de Rasinhas, Arthur da Costa Monteiro, Maria Eduarda Miranda, Milene Dias Motta, Fernando Couto Siqueira, Marilda Agudo Mendonça Teixeira de Dias, Wendell Girard Archanjo, Bráulio Soares Bozza, Patrícia T. Souza, Thiago Moreno L. Dias, Suelen Silva Gomes Soares, Vinicius Cardoso Barth, Ortrud Monika |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vieira, Debora Ferreira Barreto Silva, Marcos Alexandre Nunes da Almeida, Ana Luisa Teixeira de Rasinhas, Arthur da Costa Monteiro, Maria Eduarda Miranda, Milene Dias Motta, Fernando Couto Siqueira, Marilda Agudo Mendonça Teixeira de Dias, Wendell Girard Archanjo, Bráulio Soares Bozza, Patrícia T. Souza, Thiago Moreno L. Dias, Suelen Silva Gomes Soares, Vinicius Cardoso Barth, Ortrud Monika |
| dc.subject.other.pt_BR.fl_str_mv |
SARS-CoV-2. Células Vero-E6 Estudos ultraestruturais Morfogênese Microscopia eletrônica de transmissão Microscopia eletrônica de varredura Modelagem de microscopia eletrônica 3D |
| topic |
SARS-CoV-2. Células Vero-E6 Estudos ultraestruturais Morfogênese Microscopia eletrônica de transmissão Microscopia eletrônica de varredura Modelagem de microscopia eletrônica 3D SARS-CoV-2. Vero-E6 cells Ultrastructural studies Morphogenesis Transmission electron microscopy Scanning electron microscopy 3D electron microscopy modeling |
| dc.subject.en.pt_BR.fl_str_mv |
SARS-CoV-2. Vero-E6 cells Ultrastructural studies Morphogenesis Transmission electron microscopy Scanning electron microscopy 3D electron microscopy modeling |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. |
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2022 |
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2022-03-04T19:19:38Z |
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2022-03-04T19:19:38Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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VIEIRA, Debora ferreira Barreto et al. SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System. Viruses, v. 14, 201, p. 1 - 21, Jan. 2022. |
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https://www.arca.fiocruz.br/handle/icict/51559 |
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1999-4915 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/v14020201 |
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VIEIRA, Debora ferreira Barreto et al. SARS-CoV-2: Ultrastructural Characterization of Morphogenesis in an In Vitro System. Viruses, v. 14, 201, p. 1 - 21, Jan. 2022. 1999-4915 10.3390/v14020201 |
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