Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells

Detalhes bibliográficos
Autor(a) principal: Vieira, Debora Ferreira Barreto
Data de Publicação: 2021
Outros Autores: Silva, Marcos Alexandre Nunes da, Garcia, Cristiana Couto, Miranda, Milene Dias, Matos, Aline da Rocha, Caetano, Braulia Costa, Resende, Paola Cristina, Motta, Fernando Couto, Siqueira, Marilda Agudo Mendonça Teixeira de, Dias, Wendell Girard, Archanjo, Bráulio Soares, Barth, Ortrud Monika
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46249
Resumo: A Rede Genômica Fiocruz é formada por especialistas de todas as unidades da Fundação no país e de institutos parceiros que se empenham diariamente em gerar dados mais robustos sobre o comportamento do SARS-Cov-2 e contribuir para um melhor preparo do país no enfrentamento da pandemia em termos de diagnóstico mais precisos e vacinas eficazes. Saiba mais sobre a Rede Genômica Fiocruz em: http://www.genomahcov.fiocruz.br/
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spelling Vieira, Debora Ferreira BarretoSilva, Marcos Alexandre Nunes daGarcia, Cristiana CoutoMiranda, Milene DiasMatos, Aline da RochaCaetano, Braulia CostaResende, Paola CristinaMotta, Fernando CoutoSiqueira, Marilda Agudo Mendonça Teixeira deDias, Wendell GirardArchanjo, Bráulio SoaresBarth, Ortrud Monika2021-03-03T20:36:54Z2021-03-03T20:36:54Z2021VIEIRA, Debora Ferreira Barreto et al. Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200443, p. 1-6, 2021.0074-0276https://www.arca.fiocruz.br/handle/icict/4624910.1590/0074-027602004431678-8060A Rede Genômica Fiocruz é formada por especialistas de todas as unidades da Fundação no país e de institutos parceiros que se empenham diariamente em gerar dados mais robustos sobre o comportamento do SARS-Cov-2 e contribuir para um melhor preparo do país no enfrentamento da pandemia em termos de diagnóstico mais precisos e vacinas eficazes. Saiba mais sobre a Rede Genômica Fiocruz em: http://www.genomahcov.fiocruz.br/Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Microscopia Eletrônica Rudolph Barth. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Núcleo de Laboratórios de Microscopia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.BACKGROUND The coronaviruses (CoVs) called the attention of the world for causing outbreaks of severe acute respiratory syndrome (SARS-CoV), in Asia in 2002-03, and respiratory disease in the Middle East (MERS-CoV), in 2012. In December 2019, yet again a new coronavirus (SARS-CoV-2) first identified in Wuhan, China, was associated with a severe respiratory infection, known today as COVID-19. This new virus quickly spread throughout China and 30 additional countries. As result, the World Health Organization (WHO) elevated the status of the COVID-19 outbreak from emergency of international concern to pandemic on March 11, 2020. The impact of COVID-19 on public health and economy fueled a worldwide race to approve therapeutic and prophylactic agents, but so far, there are no specific antiviral drugs or vaccines available. In current scenario, the development of in vitro systems for viral mass production and for testing antiviral and vaccine candidates proves to be an urgent matter. OBJECTIVE The objective of this paper is study the biology of SARS-CoV-2 in Vero-E6 cells at the ultrastructural level. METHODS In this study, we documented, by transmission electron microscopy and real-time reverse transcription polymerase chain reaction (RT-PCR), the infection of Vero-E6 cells with SARS-CoV-2 samples isolated from Brazilian patients. FINDINGS The infected cells presented cytopathic effects and SARS-CoV-2 particles were observed attached to the cell surface and inside cytoplasmic vesicles. The entry of the virus into cells occurred through the endocytic pathway or by fusion of the viral envelope with the cell membrane. Assembled nucleocapsids were verified inside rough endoplasmic reticulum cisterns (RER). Viral maturation seemed to occur by budding of viral particles from the RER into smooth membrane vesicles. MAIN CONCLUSIONS Therefore, the susceptibility of Vero-E6 cells to SARS-CoV-2 infection and the viral pathway inside the cells were demonstrated by ultrastructural analysis.engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.SARS-CoV-2Células Vero-E6MorfologiaMorfogêneseRede Genômica FiocruzGENOMAHCOVSARS-CoV-2Vero-E6 cellsMorphologyMorphogenesisMorphology and morphogenesis of SARS-CoV-2 in Vero-E6 cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells
title Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells
spellingShingle Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells
Vieira, Debora Ferreira Barreto
SARS-CoV-2
Células Vero-E6
Morfologia
Morfogênese
Rede Genômica Fiocruz
GENOMAHCOV
SARS-CoV-2
Vero-E6 cells
Morphology
Morphogenesis
title_short Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells
title_full Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells
title_fullStr Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells
title_full_unstemmed Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells
title_sort Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells
author Vieira, Debora Ferreira Barreto
author_facet Vieira, Debora Ferreira Barreto
Silva, Marcos Alexandre Nunes da
Garcia, Cristiana Couto
Miranda, Milene Dias
Matos, Aline da Rocha
Caetano, Braulia Costa
Resende, Paola Cristina
Motta, Fernando Couto
Siqueira, Marilda Agudo Mendonça Teixeira de
Dias, Wendell Girard
Archanjo, Bráulio Soares
Barth, Ortrud Monika
author_role author
author2 Silva, Marcos Alexandre Nunes da
Garcia, Cristiana Couto
Miranda, Milene Dias
Matos, Aline da Rocha
Caetano, Braulia Costa
Resende, Paola Cristina
Motta, Fernando Couto
Siqueira, Marilda Agudo Mendonça Teixeira de
Dias, Wendell Girard
Archanjo, Bráulio Soares
Barth, Ortrud Monika
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Debora Ferreira Barreto
Silva, Marcos Alexandre Nunes da
Garcia, Cristiana Couto
Miranda, Milene Dias
Matos, Aline da Rocha
Caetano, Braulia Costa
Resende, Paola Cristina
Motta, Fernando Couto
Siqueira, Marilda Agudo Mendonça Teixeira de
Dias, Wendell Girard
Archanjo, Bráulio Soares
Barth, Ortrud Monika
dc.subject.other.pt_BR.fl_str_mv SARS-CoV-2
Células Vero-E6
Morfologia
Morfogênese
Rede Genômica Fiocruz
GENOMAHCOV
topic SARS-CoV-2
Células Vero-E6
Morfologia
Morfogênese
Rede Genômica Fiocruz
GENOMAHCOV
SARS-CoV-2
Vero-E6 cells
Morphology
Morphogenesis
dc.subject.en.pt_BR.fl_str_mv SARS-CoV-2
Vero-E6 cells
Morphology
Morphogenesis
description A Rede Genômica Fiocruz é formada por especialistas de todas as unidades da Fundação no país e de institutos parceiros que se empenham diariamente em gerar dados mais robustos sobre o comportamento do SARS-Cov-2 e contribuir para um melhor preparo do país no enfrentamento da pandemia em termos de diagnóstico mais precisos e vacinas eficazes. Saiba mais sobre a Rede Genômica Fiocruz em: http://www.genomahcov.fiocruz.br/
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-03-03T20:36:54Z
dc.date.available.fl_str_mv 2021-03-03T20:36:54Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIEIRA, Debora Ferreira Barreto et al. Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200443, p. 1-6, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46249
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760200443
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv VIEIRA, Debora Ferreira Barreto et al. Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200443, p. 1-6, 2021.
0074-0276
10.1590/0074-02760200443
1678-8060
url https://www.arca.fiocruz.br/handle/icict/46249
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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