Proteome profiling of human cutaneous leishmaniasis lesion.

Detalhes bibliográficos
Autor(a) principal: Santos, Claire da Silva
Data de Publicação: 2015
Outros Autores: Attarha, Sanaz, Saini, Ravi Kanth, Boaventura, Viviane, Costa, Jackson Mauricio Lopes, Cunha, Antonio Ricardo Khouri, Barral Netto, Manoel, Brodskyn, Claudia Ida, Souchelnytskyi, Serhiy
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14081
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil
id CRUZ_3eec39cc890141ff19a086801dd718a2
oai_identifier_str oai:www.arca.fiocruz.br:icict/14081
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Santos, Claire da SilvaAttarha, SanazSaini, Ravi KanthBoaventura, VivianeCosta, Jackson Mauricio LopesCunha, Antonio Ricardo KhouriBarral Netto, ManoelBrodskyn, Claudia IdaSouchelnytskyi, Serhiy2016-05-03T18:40:03Z2016-05-03T18:40:03Z2015SANTOS, C. S. et al. Proteome profiling of human cutaneous leishmaniasis lesion. Journal of Investigative Dermatology, v. 135, n. 2, p. 400-410, 2015.1523-1747https://www.arca.fiocruz.br/handle/icict/1408110.1038/jid.2014.396engElsevierProteome profiling of human cutaneous leishmaniasis lesion.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, BrasilKarolinska Institutet. Department of Oncology-Pathology. Stockholm, SwedenKarolinska Institutet. Department of Oncology-Pathology. Stockholm, SwedenUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilKarolinska Institutet. Department of Oncology-Pathology. Stockholm, SwedenIn this study, we used proteomics and biological network analysis to evaluate the potential biological processes and components present in the identified proteins of biopsies from cutaneous leishmaniasis (CL) patients infected by Leishmania braziliensis in comparison with normal skin. We identified 59 proteins differently expressed in samples from infected and normal skin. Biological network analysis employing identified proteins showed the presence of networks that may be involved in the cell death mediated by cytotoxic T lymphocytes. After immunohistochemical analyses, the expression of caspase-9, caspase-3, and granzyme B was validated in the tissue and positively correlated with the lesion size in CL patients. In conclusion, this work identified differentially expressed proteins in the inflammatory site of CL, revealed enhanced expression of caspase-9, and highlighted mechanisms associated with the progression of tissue damage observed in lesions.Leishmaniose Cutânea/metabolismoProteomaCaspase 3/análiseCaspase 9/análiseGranzimas/análiseHumanosImuno-HistoquímicaLeishmaniose Cutânea/patologiaPele/patologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14081/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos CS Proteome....pdfSantos CS Proteome....pdfapplication/pdf4508679https://www.arca.fiocruz.br/bitstream/icict/14081/2/Santos%20CS%20Proteome....pdf3b64f66a0f8908480ba80e497e9f4f98MD52TEXTSantos CS Proteome....pdf.txtSantos CS Proteome....pdf.txtExtracted texttext/plain49624https://www.arca.fiocruz.br/bitstream/icict/14081/3/Santos%20CS%20Proteome....pdf.txt7438a09954a1d10338aef4b53987ce4aMD53icict/140812023-03-15 14:34:00.6oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Proteome profiling of human cutaneous leishmaniasis lesion.
title Proteome profiling of human cutaneous leishmaniasis lesion.
spellingShingle Proteome profiling of human cutaneous leishmaniasis lesion.
Santos, Claire da Silva
Leishmaniose Cutânea/metabolismo
Proteoma
Caspase 3/análise
Caspase 9/análise
Granzimas/análise
Humanos
Imuno-Histoquímica
Leishmaniose Cutânea/patologia
Pele/patologia
title_short Proteome profiling of human cutaneous leishmaniasis lesion.
title_full Proteome profiling of human cutaneous leishmaniasis lesion.
title_fullStr Proteome profiling of human cutaneous leishmaniasis lesion.
title_full_unstemmed Proteome profiling of human cutaneous leishmaniasis lesion.
title_sort Proteome profiling of human cutaneous leishmaniasis lesion.
author Santos, Claire da Silva
author_facet Santos, Claire da Silva
Attarha, Sanaz
Saini, Ravi Kanth
Boaventura, Viviane
Costa, Jackson Mauricio Lopes
Cunha, Antonio Ricardo Khouri
Barral Netto, Manoel
Brodskyn, Claudia Ida
Souchelnytskyi, Serhiy
author_role author
author2 Attarha, Sanaz
Saini, Ravi Kanth
Boaventura, Viviane
Costa, Jackson Mauricio Lopes
Cunha, Antonio Ricardo Khouri
Barral Netto, Manoel
Brodskyn, Claudia Ida
Souchelnytskyi, Serhiy
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Claire da Silva
Attarha, Sanaz
Saini, Ravi Kanth
Boaventura, Viviane
Costa, Jackson Mauricio Lopes
Cunha, Antonio Ricardo Khouri
Barral Netto, Manoel
Brodskyn, Claudia Ida
Souchelnytskyi, Serhiy
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose Cutânea/metabolismo
Proteoma
Caspase 3/análise
Caspase 9/análise
Granzimas/análise
Humanos
Imuno-Histoquímica
Leishmaniose Cutânea/patologia
Pele/patologia
topic Leishmaniose Cutânea/metabolismo
Proteoma
Caspase 3/análise
Caspase 9/análise
Granzimas/análise
Humanos
Imuno-Histoquímica
Leishmaniose Cutânea/patologia
Pele/patologia
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-05-03T18:40:03Z
dc.date.available.fl_str_mv 2016-05-03T18:40:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, C. S. et al. Proteome profiling of human cutaneous leishmaniasis lesion. Journal of Investigative Dermatology, v. 135, n. 2, p. 400-410, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14081
dc.identifier.issn.none.fl_str_mv 1523-1747
dc.identifier.doi.none.fl_str_mv 10.1038/jid.2014.396
identifier_str_mv SANTOS, C. S. et al. Proteome profiling of human cutaneous leishmaniasis lesion. Journal of Investigative Dermatology, v. 135, n. 2, p. 400-410, 2015.
1523-1747
10.1038/jid.2014.396
url https://www.arca.fiocruz.br/handle/icict/14081
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/14081/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/14081/2/Santos%20CS%20Proteome....pdf
https://www.arca.fiocruz.br/bitstream/icict/14081/3/Santos%20CS%20Proteome....pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
3b64f66a0f8908480ba80e497e9f4f98
7438a09954a1d10338aef4b53987ce4a
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324806944292864

Registros relacionados