Proteome profiling of human cutaneous Leishmaniasis lesion
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8708 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
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Santos, Claire da SilvaAttarha, SanazSaini, Ravi KanthBoaventura, Viviane SampaioCosta, Jackson Mauricio LopesCunha, Antonio Ricardo KhouriBarral Netto, ManoelBrodskyn, Claudia IdaSouchelnytskyi, Serhiy2014-10-31T18:56:48Z2014-10-31T18:56:48Z2014SANTOS, C. S. et al. Proteome profiling of human cutaneous Leishmaniasis lesion. Journal of Investigative Dermatology, oct. 2014.0022-202Xhttps://www.arca.fiocruz.br/handle/icict/870810.1038/jid.2014.396engNature Publishing GroupProcessos biológicosLeishmaniose cutâneaLeishmania braziliensisBiópsiaInflamaçãoCaspase 9Proteome profiling of human cutaneous Leishmaniasis lesioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilKarolinska Institutet. Department of Oncology-Pathology. Stockholm, SwedenKarolinska Institutet. Department of Oncology-Pathology. Stockholm, SwedenUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilKarolinska Institutet. Department of Oncology-Pathology. Stockholm, SwedenIn this study, we used proteomics and biological network analysis to evaluate the potential biological processes and components present in the identified proteins of biopsies from cutaneous leishmaniasis (CL) patients infected by Leishmania braziliensis in comparison with normal skin. We identified 59 proteins differently expressed in samples from infected and normal skin. Biological network analysis employing identified proteins showed the presence of networks that may be involved in the cell death mediated by cytotoxic T lymphocytes. After immunohistochemical analyses, the expression of caspase-9, caspase-3, and granzyme B was validated in the tissue and positively correlated with the lesion size in CL patients. In conclusion, this work identified differentially expressed proteins in the inflammatory site of CL, revealed enhanced expression of caspase-9, and highlighted mechanisms associated with the progression of tissue damage observed in lesions.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8708/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALSantos CS Proteome....pdfSantos CS Proteome....pdfapplication/pdf2759836https://www.arca.fiocruz.br/bitstream/icict/8708/2/Santos%20CS%20Proteome....pdf3f21ab5e83f2f123f27989a7c07d9bc9MD52TEXTSantos CS Proteome....pdf.txtSantos CS Proteome....pdf.txtExtracted texttext/plain49515https://www.arca.fiocruz.br/bitstream/icict/8708/3/Santos%20CS%20Proteome....pdf.txt98dff1f0177834033ca7fb3a4f362d47MD53icict/87082023-03-15 14:32:59.786oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Proteome profiling of human cutaneous Leishmaniasis lesion |
| title |
Proteome profiling of human cutaneous Leishmaniasis lesion |
| spellingShingle |
Proteome profiling of human cutaneous Leishmaniasis lesion Santos, Claire da Silva Processos biológicos Leishmaniose cutânea Leishmania braziliensis Biópsia Inflamação Caspase 9 |
| title_short |
Proteome profiling of human cutaneous Leishmaniasis lesion |
| title_full |
Proteome profiling of human cutaneous Leishmaniasis lesion |
| title_fullStr |
Proteome profiling of human cutaneous Leishmaniasis lesion |
| title_full_unstemmed |
Proteome profiling of human cutaneous Leishmaniasis lesion |
| title_sort |
Proteome profiling of human cutaneous Leishmaniasis lesion |
| author |
Santos, Claire da Silva |
| author_facet |
Santos, Claire da Silva Attarha, Sanaz Saini, Ravi Kanth Boaventura, Viviane Sampaio Costa, Jackson Mauricio Lopes Cunha, Antonio Ricardo Khouri Barral Netto, Manoel Brodskyn, Claudia Ida Souchelnytskyi, Serhiy |
| author_role |
author |
| author2 |
Attarha, Sanaz Saini, Ravi Kanth Boaventura, Viviane Sampaio Costa, Jackson Mauricio Lopes Cunha, Antonio Ricardo Khouri Barral Netto, Manoel Brodskyn, Claudia Ida Souchelnytskyi, Serhiy |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Claire da Silva Attarha, Sanaz Saini, Ravi Kanth Boaventura, Viviane Sampaio Costa, Jackson Mauricio Lopes Cunha, Antonio Ricardo Khouri Barral Netto, Manoel Brodskyn, Claudia Ida Souchelnytskyi, Serhiy |
| dc.subject.other.pt_BR.fl_str_mv |
Processos biológicos Leishmaniose cutânea Leishmania braziliensis Biópsia Inflamação Caspase 9 |
| topic |
Processos biológicos Leishmaniose cutânea Leishmania braziliensis Biópsia Inflamação Caspase 9 |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
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2014 |
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2014-10-31T18:56:48Z |
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2014-10-31T18:56:48Z |
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2014 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SANTOS, C. S. et al. Proteome profiling of human cutaneous Leishmaniasis lesion. Journal of Investigative Dermatology, oct. 2014. |
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https://www.arca.fiocruz.br/handle/icict/8708 |
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0022-202X |
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10.1038/jid.2014.396 |
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SANTOS, C. S. et al. Proteome profiling of human cutaneous Leishmaniasis lesion. Journal of Investigative Dermatology, oct. 2014. 0022-202X 10.1038/jid.2014.396 |
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Nature Publishing Group |
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