Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones

Detalhes bibliográficos
Autor(a) principal: Arenhart, Sandra
Data de Publicação: 2016
Outros Autores: Silva, José Valter Joaquim, Flores, Eduardo Furtado, Weiblen, Rudi, Gil, Laura Helena Vega Gonzales
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19299
Resumo: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brazil / Universidade Federal de Santa Maria. Centro de Ciências Rurais (CCR). Departamento de Medicina Veterinária Preventiva (DMVP). Santa Maria, RS, Brazil.
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spelling Arenhart, SandraSilva, José Valter JoaquimFlores, Eduardo FurtadoWeiblen, RudiGil, Laura Helena Vega Gonzales2017-06-05T14:48:35Z2017-06-05T14:48:35Z2016ARENHART, S. et al. Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones. Brazilian Journal of Microbiology, v. 47, n. 4, p. 993–999, out. 2016.1678-4405https://www.arca.fiocruz.br/handle/icict/1929910.1016/j.bjm.2016.07.022engUse of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clonesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brazil / Universidade Federal de Santa Maria. Centro de Ciências Rurais (CCR). Departamento de Medicina Veterinária Preventiva (DMVP). Santa Maria, RS, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brazil.Universidade Federal de Santa Maria. Centro de Ciências Rurais (CCR). Departamento de Medicina Veterinária Preventiva (DMVP). Santa Maria, RS, Brazil.Universidade Federal de Santa Maria. Centro de Ciências Rurais (CCR). Departamento de Medicina Veterinária Preventiva (DMVP). Santa Maria, RS, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brazil.The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and chi-NADL/IBSP4ncp#3). The recombinant clones were successfully recovered, resulting in viable viruses, having the kinetics of replication, focus size, and morphology similar to those of the parental virus, IBSP4ncp. In addition, the chimeric viruses remained stable for at least 10 passages in cell culture, maintaining their replication efficiency unaltered. Nucleotide sequencing revealed a few point mutations; nevertheless, the phenotype of the rescued viruses was nearly identical to that of the parental virus in all experiments. Thus, genetic stability of the chimeric clones and their phenotypic similarity to the parental virus confirm the ability of the yeast-based homologous recombination to maintain characteristics of the parental virus from which the recombinant viruses were derived. The data also support possible use of the yeast system for the manipulation of the BVDV genome.DNA ComplementarDiarreia Vírus, Bovine Viral / genéticaGenoma ViralRecombinação homólogaLeveduras / genéticaAnálise de Sequência, DNAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19299/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL27522929 2016 are-use.oa.pdf27522929 2016 are-use.oa.pdfapplication/pdf989966https://www.arca.fiocruz.br/bitstream/icict/19299/2/27522929%202016%20are-use.oa.pdfa80937a22b2590d05bdcb15aec496b74MD52TEXT27522929 2016 are-use.oa.pdf.txt27522929 2016 are-use.oa.pdf.txtExtracted texttext/plain36259https://www.arca.fiocruz.br/bitstream/icict/19299/3/27522929%202016%20are-use.oa.pdf.txt82a225e40647f7269cf1cd71e4dc12afMD53icict/192992019-04-02 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dc.title.pt_BR.fl_str_mv Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
title Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
spellingShingle Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
Arenhart, Sandra
DNA Complementar
Diarreia Vírus, Bovine Viral / genética
Genoma Viral
Recombinação homóloga
Leveduras / genética
Análise de Sequência, DNA
title_short Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
title_full Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
title_fullStr Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
title_full_unstemmed Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
title_sort Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
author Arenhart, Sandra
author_facet Arenhart, Sandra
Silva, José Valter Joaquim
Flores, Eduardo Furtado
Weiblen, Rudi
Gil, Laura Helena Vega Gonzales
author_role author
author2 Silva, José Valter Joaquim
Flores, Eduardo Furtado
Weiblen, Rudi
Gil, Laura Helena Vega Gonzales
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Arenhart, Sandra
Silva, José Valter Joaquim
Flores, Eduardo Furtado
Weiblen, Rudi
Gil, Laura Helena Vega Gonzales
dc.subject.decs.none.fl_str_mv DNA Complementar
Diarreia Vírus, Bovine Viral / genética
Genoma Viral
Recombinação homóloga
Leveduras / genética
Análise de Sequência, DNA
topic DNA Complementar
Diarreia Vírus, Bovine Viral / genética
Genoma Viral
Recombinação homóloga
Leveduras / genética
Análise de Sequência, DNA
description Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brazil / Universidade Federal de Santa Maria. Centro de Ciências Rurais (CCR). Departamento de Medicina Veterinária Preventiva (DMVP). Santa Maria, RS, Brazil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-06-05T14:48:35Z
dc.date.available.fl_str_mv 2017-06-05T14:48:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARENHART, S. et al. Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones. Brazilian Journal of Microbiology, v. 47, n. 4, p. 993–999, out. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19299
dc.identifier.issn.pt_BR.fl_str_mv 1678-4405
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.bjm.2016.07.022
identifier_str_mv ARENHART, S. et al. Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones. Brazilian Journal of Microbiology, v. 47, n. 4, p. 993–999, out. 2016.
1678-4405
10.1016/j.bjm.2016.07.022
url https://www.arca.fiocruz.br/handle/icict/19299
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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