Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/1877 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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Novais, Fernanda OliveiraSantiago, Rômulo CarvalhoBafica, Andre Luiz BarbosaCunha, Antonio Ricardo KhouriAfonso, Lilian MariaBorges, Valeria de MatosBrodskyn, Claudia IdaBarral Netto, ManoelBarral, Aldina Maria PradoOliveira, Camila Indiani de2011-04-06T15:23:05Z2011-04-06T15:23:05Z2009NOVAIS, F. O. Neutrophilis and macrophages cooperate in host resistance against Leishmania braziliensis infection. The Journal of Immunology, v. 183, n. 12, p. 8088-8098, 2009.1550-6606https://www.arca.fiocruz.br/handle/icict/187710.4049/jimmunol.0803720engNeutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Florianópolis, SC, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, EspanhaFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilNeutrophils play an active role in the control of infections caused by intracellular pathogens such as Leishmania. In the present study, we investigated the effect of neutrophil depletion at the time of Leishmania braziliensis infection of BALB/c mice and how neutrophils interact with the infected macrophage to promote parasite elimination. The in vivo depletion of neutrophils led to a significant increase in parasite load and enhanced the Th1-Th2 immune response in this experimental model of infection. BALB/c mice coinoculated with both parasites and live neutrophils displayed lower parasite burdens at the site of infection and in the draining lymph nodes. In vitro, we observed that live neutrophils significantly reduced the parasite load in L. braziliensis-infected murine macrophages, an effect not observed with Leishmania major. L. braziliensis elimination was dependent on the interaction between neutrophils and macrophages and was associated with TNF-alpha as well as superoxide production. Furthermore, cooperation between neutrophils and macrophages toward parasite elimination was also observed in experiments performed with L. braziliensis-infected human cells and, importantly, with two other New World Leishmania species. These results indicate that neutrophils play an important and previously unappreciated role in L. braziliensis infection, favoring the induction of a protective immune response.Leishmaniose Tegumentar DifusaLeishmania braziliensisImunidade InataComunicação CelularMacrófagos PeritoneaisNeutrófilos/IMinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNeutrophils and Macrophages Cooperate in Host.pdfNeutrophils and Macrophages Cooperate in Host.pdfapplication/pdf4388799https://www.arca.fiocruz.br/bitstream/icict/1877/1/Neutrophils%20and%20Macrophages%20Cooperate%20in%20Host.pdff0bddd5e7ee078ade756c94dceaa464cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/1877/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTNeutrophils and Macrophages Cooperate in Host.pdf.txtNeutrophils and Macrophages Cooperate in Host.pdf.txtExtracted texttext/plain64136https://www.arca.fiocruz.br/bitstream/icict/1877/5/Neutrophils%20and%20Macrophages%20Cooperate%20in%20Host.pdf.txtf5b4b57b83dff02e68c80f0414c877a5MD55THUMBNAILNeutrophils and Macrophages Cooperate in Host.pdf.jpgNeutrophils and Macrophages Cooperate in Host.pdf.jpgGenerated Thumbnailimage/jpeg2362https://www.arca.fiocruz.br/bitstream/icict/1877/4/Neutrophils%20and%20Macrophages%20Cooperate%20in%20Host.pdf.jpg2d9406c62f23500531077a23fd04971cMD54icict/18772023-03-15 14:33:23.957oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection |
title |
Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection |
spellingShingle |
Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection Novais, Fernanda Oliveira Leishmaniose Tegumentar Difusa Leishmania braziliensis Imunidade Inata Comunicação Celular Macrófagos Peritoneais Neutrófilos/IM |
title_short |
Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection |
title_full |
Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection |
title_fullStr |
Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection |
title_full_unstemmed |
Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection |
title_sort |
Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection |
author |
Novais, Fernanda Oliveira |
author_facet |
Novais, Fernanda Oliveira Santiago, Rômulo Carvalho Bafica, Andre Luiz Barbosa Cunha, Antonio Ricardo Khouri Afonso, Lilian Maria Borges, Valeria de Matos Brodskyn, Claudia Ida Barral Netto, Manoel Barral, Aldina Maria Prado Oliveira, Camila Indiani de |
author_role |
author |
author2 |
Santiago, Rômulo Carvalho Bafica, Andre Luiz Barbosa Cunha, Antonio Ricardo Khouri Afonso, Lilian Maria Borges, Valeria de Matos Brodskyn, Claudia Ida Barral Netto, Manoel Barral, Aldina Maria Prado Oliveira, Camila Indiani de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Novais, Fernanda Oliveira Santiago, Rômulo Carvalho Bafica, Andre Luiz Barbosa Cunha, Antonio Ricardo Khouri Afonso, Lilian Maria Borges, Valeria de Matos Brodskyn, Claudia Ida Barral Netto, Manoel Barral, Aldina Maria Prado Oliveira, Camila Indiani de |
dc.subject.decs.pt_BR.fl_str_mv |
Leishmaniose Tegumentar Difusa Leishmania braziliensis Imunidade Inata Comunicação Celular Macrófagos Peritoneais Neutrófilos/IM |
topic |
Leishmaniose Tegumentar Difusa Leishmania braziliensis Imunidade Inata Comunicação Celular Macrófagos Peritoneais Neutrófilos/IM |
description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2011-04-06T15:23:05Z |
dc.date.available.fl_str_mv |
2011-04-06T15:23:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NOVAIS, F. O. Neutrophilis and macrophages cooperate in host resistance against Leishmania braziliensis infection. The Journal of Immunology, v. 183, n. 12, p. 8088-8098, 2009. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/1877 |
dc.identifier.issn.none.fl_str_mv |
1550-6606 |
dc.identifier.doi.none.fl_str_mv |
10.4049/jimmunol.0803720 |
identifier_str_mv |
NOVAIS, F. O. Neutrophilis and macrophages cooperate in host resistance against Leishmania braziliensis infection. The Journal of Immunology, v. 183, n. 12, p. 8088-8098, 2009. 1550-6606 10.4049/jimmunol.0803720 |
url |
https://www.arca.fiocruz.br/handle/icict/1877 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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