Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection

Detalhes bibliográficos
Autor(a) principal: Novais, Fernanda Oliveira
Data de Publicação: 2009
Outros Autores: Santiago, Rômulo Carvalho, Bafica, Andre Luiz Barbosa, Cunha, Antonio Ricardo Khouri, Afonso, Lilian Maria, Borges, Valeria de Matos, Brodskyn, Claudia Ida, Barral Netto, Manoel, Barral, Aldina Maria Prado, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/1877
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Novais, Fernanda OliveiraSantiago, Rômulo CarvalhoBafica, Andre Luiz BarbosaCunha, Antonio Ricardo KhouriAfonso, Lilian MariaBorges, Valeria de MatosBrodskyn, Claudia IdaBarral Netto, ManoelBarral, Aldina Maria PradoOliveira, Camila Indiani de2011-04-06T15:23:05Z2011-04-06T15:23:05Z2009NOVAIS, F. O. Neutrophilis and macrophages cooperate in host resistance against Leishmania braziliensis infection. The Journal of Immunology, v. 183, n. 12, p. 8088-8098, 2009.1550-6606https://www.arca.fiocruz.br/handle/icict/187710.4049/​jimmunol.0803720engNeutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Florianópolis, SC, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, EspanhaFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilNeutrophils play an active role in the control of infections caused by intracellular pathogens such as Leishmania. In the present study, we investigated the effect of neutrophil depletion at the time of Leishmania braziliensis infection of BALB/c mice and how neutrophils interact with the infected macrophage to promote parasite elimination. The in vivo depletion of neutrophils led to a significant increase in parasite load and enhanced the Th1-Th2 immune response in this experimental model of infection. BALB/c mice coinoculated with both parasites and live neutrophils displayed lower parasite burdens at the site of infection and in the draining lymph nodes. In vitro, we observed that live neutrophils significantly reduced the parasite load in L. braziliensis-infected murine macrophages, an effect not observed with Leishmania major. L. braziliensis elimination was dependent on the interaction between neutrophils and macrophages and was associated with TNF-alpha as well as superoxide production. Furthermore, cooperation between neutrophils and macrophages toward parasite elimination was also observed in experiments performed with L. braziliensis-infected human cells and, importantly, with two other New World Leishmania species. These results indicate that neutrophils play an important and previously unappreciated role in L. braziliensis infection, favoring the induction of a protective immune response.Leishmaniose Tegumentar DifusaLeishmania braziliensisImunidade InataComunicação CelularMacrófagos PeritoneaisNeutrófilos/IMinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNeutrophils and Macrophages Cooperate in Host.pdfNeutrophils and Macrophages Cooperate in Host.pdfapplication/pdf4388799https://www.arca.fiocruz.br/bitstream/icict/1877/1/Neutrophils%20and%20Macrophages%20Cooperate%20in%20Host.pdff0bddd5e7ee078ade756c94dceaa464cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/1877/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTNeutrophils and Macrophages Cooperate in Host.pdf.txtNeutrophils and Macrophages Cooperate in Host.pdf.txtExtracted texttext/plain64136https://www.arca.fiocruz.br/bitstream/icict/1877/5/Neutrophils%20and%20Macrophages%20Cooperate%20in%20Host.pdf.txtf5b4b57b83dff02e68c80f0414c877a5MD55THUMBNAILNeutrophils and Macrophages Cooperate in Host.pdf.jpgNeutrophils and Macrophages Cooperate in Host.pdf.jpgGenerated Thumbnailimage/jpeg2362https://www.arca.fiocruz.br/bitstream/icict/1877/4/Neutrophils%20and%20Macrophages%20Cooperate%20in%20Host.pdf.jpg2d9406c62f23500531077a23fd04971cMD54icict/18772023-03-15 14:33:23.957oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection
title Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection
spellingShingle Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection
Novais, Fernanda Oliveira
Leishmaniose Tegumentar Difusa
Leishmania braziliensis
Imunidade Inata
Comunicação Celular
Macrófagos Peritoneais
Neutrófilos/IM
title_short Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection
title_full Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection
title_fullStr Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection
title_full_unstemmed Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection
title_sort Neutrophils and macrophages cooperate in host resistance against Leishmania braziliensis infection
author Novais, Fernanda Oliveira
author_facet Novais, Fernanda Oliveira
Santiago, Rômulo Carvalho
Bafica, Andre Luiz Barbosa
Cunha, Antonio Ricardo Khouri
Afonso, Lilian Maria
Borges, Valeria de Matos
Brodskyn, Claudia Ida
Barral Netto, Manoel
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
author_role author
author2 Santiago, Rômulo Carvalho
Bafica, Andre Luiz Barbosa
Cunha, Antonio Ricardo Khouri
Afonso, Lilian Maria
Borges, Valeria de Matos
Brodskyn, Claudia Ida
Barral Netto, Manoel
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Novais, Fernanda Oliveira
Santiago, Rômulo Carvalho
Bafica, Andre Luiz Barbosa
Cunha, Antonio Ricardo Khouri
Afonso, Lilian Maria
Borges, Valeria de Matos
Brodskyn, Claudia Ida
Barral Netto, Manoel
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose Tegumentar Difusa
Leishmania braziliensis
Imunidade Inata
Comunicação Celular
Macrófagos Peritoneais
Neutrófilos/IM
topic Leishmaniose Tegumentar Difusa
Leishmania braziliensis
Imunidade Inata
Comunicação Celular
Macrófagos Peritoneais
Neutrófilos/IM
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2011-04-06T15:23:05Z
dc.date.available.fl_str_mv 2011-04-06T15:23:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv NOVAIS, F. O. Neutrophilis and macrophages cooperate in host resistance against Leishmania braziliensis infection. The Journal of Immunology, v. 183, n. 12, p. 8088-8098, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/1877
dc.identifier.issn.none.fl_str_mv 1550-6606
dc.identifier.doi.none.fl_str_mv 10.4049/​jimmunol.0803720
identifier_str_mv NOVAIS, F. O. Neutrophilis and macrophages cooperate in host resistance against Leishmania braziliensis infection. The Journal of Immunology, v. 183, n. 12, p. 8088-8098, 2009.
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