Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23937 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-470453 / 2012-5). |
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Kassar, Telissa C.Magalhães, TerezaS., José V. J.Carvalho, Amanda G. O.Silva, Andréa N. M. R. daQueiroz, Sabrina R. A.Bertani, Giovani R.Gil, Laura H. V. G.2018-01-17T14:47:40Z2018-01-17T14:47:40Z2017KASSAR, T. C. et al. Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase. Anais da Academia Brasileira de Ciencias, v. 89, n. 3 Suppl, p. 2119–2130, 2017.1678-2690https://www.arca.fiocruz.br/handle/icict/2393710.1590/0001-3765201720160196Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-470453 / 2012-5).Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Bioquímica. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct.engGaussia luciferaserecombinação homóloga em levedurasgene repórtervírus da febre amarelaGaussia luciferasehomologous recombination in yeastreporter geneyellow fever virusfebre amarelaEstudos TransversaisBrasilepidemiologiasaúde públicaConstruction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23937/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALIAM - Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase.pdfIAM - Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase.pdfapplication/pdf1744508https://www.arca.fiocruz.br/bitstream/icict/23937/2/IAM%20-%20Construction%20and%20characterization%20of%20a%20recombinant%20yellow%20fever%20virus%20stably%20expressing%20Gaussia%20luciferase.pdfda44e9a7e3b2c7e91aa652ad0b067eeaMD52TEXTIAM - Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase.pdf.txtIAM - Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase.pdf.txtExtracted texttext/plain39190https://www.arca.fiocruz.br/bitstream/icict/23937/3/IAM%20-%20Construction%20and%20characterization%20of%20a%20recombinant%20yellow%20fever%20virus%20stably%20expressing%20Gaussia%20luciferase.pdf.txta464e1d4b182c7ac93a414eb06ffb7f3MD53icict/239372018-08-15 02:10:08.535oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-08-15T05:10:08Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.pt_BR.fl_str_mv |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| title |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| spellingShingle |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase Kassar, Telissa C. Gaussia luciferase recombinação homóloga em leveduras gene repórter vírus da febre amarela Gaussia luciferase homologous recombination in yeast reporter gene yellow fever virus febre amarela Estudos Transversais Brasil epidemiologia saúde pública |
| title_short |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| title_full |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| title_fullStr |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| title_full_unstemmed |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| title_sort |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| author |
Kassar, Telissa C. |
| author_facet |
Kassar, Telissa C. Magalhães, Tereza S., José V. J. Carvalho, Amanda G. O. Silva, Andréa N. M. R. da Queiroz, Sabrina R. A. Bertani, Giovani R. Gil, Laura H. V. G. |
| author_role |
author |
| author2 |
Magalhães, Tereza S., José V. J. Carvalho, Amanda G. O. Silva, Andréa N. M. R. da Queiroz, Sabrina R. A. Bertani, Giovani R. Gil, Laura H. V. G. |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Kassar, Telissa C. Magalhães, Tereza S., José V. J. Carvalho, Amanda G. O. Silva, Andréa N. M. R. da Queiroz, Sabrina R. A. Bertani, Giovani R. Gil, Laura H. V. G. |
| dc.subject.other.pt_BR.fl_str_mv |
Gaussia luciferase recombinação homóloga em leveduras gene repórter vírus da febre amarela |
| topic |
Gaussia luciferase recombinação homóloga em leveduras gene repórter vírus da febre amarela Gaussia luciferase homologous recombination in yeast reporter gene yellow fever virus febre amarela Estudos Transversais Brasil epidemiologia saúde pública |
| dc.subject.en.pt_BR.fl_str_mv |
Gaussia luciferase homologous recombination in yeast reporter gene yellow fever virus |
| dc.subject.decs.pt_BR.fl_str_mv |
febre amarela Estudos Transversais Brasil epidemiologia saúde pública |
| description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-470453 / 2012-5). |
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2017 |
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2017 |
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2018-01-17T14:47:40Z |
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2018-01-17T14:47:40Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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KASSAR, T. C. et al. Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase. Anais da Academia Brasileira de Ciencias, v. 89, n. 3 Suppl, p. 2119–2130, 2017. |
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https://www.arca.fiocruz.br/handle/icict/23937 |
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1678-2690 |
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10.1590/0001-3765201720160196 |
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KASSAR, T. C. et al. Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase. Anais da Academia Brasileira de Ciencias, v. 89, n. 3 Suppl, p. 2119–2130, 2017. 1678-2690 10.1590/0001-3765201720160196 |
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