High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers

Detalhes bibliográficos
Autor(a) principal: Lima, Luciana Sampaio
Data de Publicação: 2022
Outros Autores: Proietti-Junior, Aldo Aparecido, Rodrigues, Yan Corrêa, Vieira, Marcelo Cleyton da Silva, Lima, Luana Nepomuceno Gondim Costa, Souza, Cintya de Oliveira, Gonçalves, Verônica Dias, Lima, Marcelo de Oliveira, Rodrigues, Dália dos Prazeres, Lima, Karla Valéria Batista
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/53547
Resumo: Universidade Federal do Amapá. Faculdade de Ciências Farmacêuticas. Macapá, AP, Brasil.
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spelling Lima, Luciana SampaioProietti-Junior, Aldo AparecidoRodrigues, Yan CorrêaVieira, Marcelo Cleyton da SilvaLima, Luana Nepomuceno Gondim CostaSouza, Cintya de OliveiraGonçalves, Verônica DiasLima, Marcelo de OliveiraRodrigues, Dália dos PrazeresLima, Karla Valéria Batista2022-06-27T20:16:06Z2022-06-27T20:16:06Z2022LIMA, Luciana Sampaio et al. High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers. Microorganisms, v. 10, 808, p. 1 - 16, Apr. 2022.2076-2607https://www.arca.fiocruz.br/handle/icict/5354710.3390/ microorganisms10040808engMDPIResistência a quinolonasResistência antimicrobianaDiversidade genéticaEpidemiologia molecularAnimais produtores de alimentosPirarucuRegião amazônicaQuinolone-resistanceAntimicrobial resistanceESBLsPMQRGenetic diversityMolecular epidemiologyFood-producing animalsPirarucuAmazon regionHigh Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Riversinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Amapá. Faculdade de Ciências Farmacêuticas. Macapá, AP, Brasil.Universidade Federal do Amapá. Faculdade de Ciências Farmacêuticas. Macapá, AP, Brasil.Ministério da Saúde. Instituto Evandro Chagas. Secretaria de Vigilância em Saúde. Seção de Bacteriologia e Micologia. Ananindeua, PA, Brasil.Ministério da Saúde. Instituto Evandro Chagas. Secretaria de Vigilância em Saúde. Seção de Bacteriologia e Micologia. Ananindeua, PA, Brasil.Ministério da Saúde. Instituto Evandro Chagas. Secretaria de Vigilância em Saúde. Seção de Bacteriologia e Micologia. Ananindeua, PA, Brasil.Ministério da Saúde. Instituto Evandro Chagas. Secretaria de Vigilância em Saúde. Seção de Bacteriologia e Micologia. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Instituto Evandro Chagas. Secretaria de Vigilância em Saúde. Seção do Meio Ambiente. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ, Brasil.The increasing prevalence of multi-drug resistant (MDR) Escherichia coli in distinct ecological niches, comprising water sources and food-producing animals, such as fish species, has been widely reported. In the present study, quinolone-resistant E. coli isolates from Arapirama gigas, a major fish species in the Brazilian Amazon rivers and fish farms, were characterized regarding their antimicrobial susceptibility, virulence, and genetic diversity. A total of forty (40) specimens of A. gigas, including 20 farmed and 20 wild fish, were included. Thirty-four quinolone-resistant E. coli isolates were phenotypically tested by broth microdilution, while resistance and virulence genes were detected by PCR. Molecular epidemiology and genetic relatedness were analyzed by MLST and PFGE typing. The majority of isolates were classified as MDR and detected harboring blaCTX-M, qnrA and qnrB genes. Enterotoxigenic E. coli pathotype (ETEC) isolates were presented in low prevalence among farmed animals. MLST and PFGE genotyping revealed a wide genetic background, including the detection of internationally spread clones. The obtained data point out A. gigas as a reservoir in Brazilian Amazon aquatic ecosystems and warns of the interference of AMR strains in wildlife and environmental matrices.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/53547/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDaliaPRodrigues_VeronicaGonçalves_etal_IOC_2022.pdfDaliaPRodrigues_VeronicaGonçalves_etal_IOC_2022.pdfapplication/pdf835481https://www.arca.fiocruz.br/bitstream/icict/53547/2/DaliaPRodrigues_VeronicaGon%c3%a7alves_etal_IOC_2022.pdfc43b4d1bba22395b8468b1f61194dc00MD52icict/535472022-06-27 17:16:06.693oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-27T20:16:06Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.pt_BR.fl_str_mv High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers
title High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers
spellingShingle High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers
Lima, Luciana Sampaio
Resistência a quinolonas
Resistência antimicrobiana
Diversidade genética
Epidemiologia molecular
Animais produtores de alimentos
Pirarucu
Região amazônica
Quinolone-resistance
Antimicrobial resistance
ESBLs
PMQR
Genetic diversity
Molecular epidemiology
Food-producing animals
Pirarucu
Amazon region
title_short High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers
title_full High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers
title_fullStr High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers
title_full_unstemmed High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers
title_sort High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers
author Lima, Luciana Sampaio
author_facet Lima, Luciana Sampaio
Proietti-Junior, Aldo Aparecido
Rodrigues, Yan Corrêa
Vieira, Marcelo Cleyton da Silva
Lima, Luana Nepomuceno Gondim Costa
Souza, Cintya de Oliveira
Gonçalves, Verônica Dias
Lima, Marcelo de Oliveira
Rodrigues, Dália dos Prazeres
Lima, Karla Valéria Batista
author_role author
author2 Proietti-Junior, Aldo Aparecido
Rodrigues, Yan Corrêa
Vieira, Marcelo Cleyton da Silva
Lima, Luana Nepomuceno Gondim Costa
Souza, Cintya de Oliveira
Gonçalves, Verônica Dias
Lima, Marcelo de Oliveira
Rodrigues, Dália dos Prazeres
Lima, Karla Valéria Batista
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, Luciana Sampaio
Proietti-Junior, Aldo Aparecido
Rodrigues, Yan Corrêa
Vieira, Marcelo Cleyton da Silva
Lima, Luana Nepomuceno Gondim Costa
Souza, Cintya de Oliveira
Gonçalves, Verônica Dias
Lima, Marcelo de Oliveira
Rodrigues, Dália dos Prazeres
Lima, Karla Valéria Batista
dc.subject.other.pt_BR.fl_str_mv Resistência a quinolonas
Resistência antimicrobiana
Diversidade genética
Epidemiologia molecular
Animais produtores de alimentos
Pirarucu
Região amazônica
topic Resistência a quinolonas
Resistência antimicrobiana
Diversidade genética
Epidemiologia molecular
Animais produtores de alimentos
Pirarucu
Região amazônica
Quinolone-resistance
Antimicrobial resistance
ESBLs
PMQR
Genetic diversity
Molecular epidemiology
Food-producing animals
Pirarucu
Amazon region
dc.subject.en.pt_BR.fl_str_mv Quinolone-resistance
Antimicrobial resistance
ESBLs
PMQR
Genetic diversity
Molecular epidemiology
Food-producing animals
Pirarucu
Amazon region
description Universidade Federal do Amapá. Faculdade de Ciências Farmacêuticas. Macapá, AP, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-06-27T20:16:06Z
dc.date.available.fl_str_mv 2022-06-27T20:16:06Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Luciana Sampaio et al. High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers. Microorganisms, v. 10, 808, p. 1 - 16, Apr. 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/53547
dc.identifier.issn.pt_BR.fl_str_mv 2076-2607
dc.identifier.doi.none.fl_str_mv 10.3390/ microorganisms10040808
identifier_str_mv LIMA, Luciana Sampaio et al. High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers. Microorganisms, v. 10, 808, p. 1 - 16, Apr. 2022.
2076-2607
10.3390/ microorganisms10040808
url https://www.arca.fiocruz.br/handle/icict/53547
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/53547/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/53547/2/DaliaPRodrigues_VeronicaGon%c3%a7alves_etal_IOC_2022.pdf
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