High genetic diversity and antimicrobial resistance in Escherichia coli highlight Arapaima gigas (Pisces: Arapaimidae) as a reservoir of quinolone-resistant strains in Brazilian Amazon rivers

• Autor(a) principal: Lima, Luciana Sampaio
• Data de Publicação: 2022
• Outros Autores: Proietti Junior, Aldo Aparecido, Rodrigues, Yan Corrêa, Vieira, Marcelo Cleyton da Silva, Lima, Luana Nepomuceno Gondim Costa, Souza, Cintya de Oliveira, Gonçalves, Verônica Dias, Lima, Marcelo de Oliveira, Rodrigues, Dália dos Prazeres, Lima, Karla Valéria Batista
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: http://patua.iec.gov.br//handle/iec/4541
• topic: Peixes / anatomia & histologia; Escherichia coli / patogenicidade; Ecossistema Amazônico; Arapirama gigas; Quinolonas; Resistência Microbiana a Medicamentos

The increasing prevalence of multi-drug resistant (MDR) Escherichia coli in distinct ecological niches, comprising water sources and food-producing animals, such as fish species, has been widely reported. In the present study, quinolone-resistant E. coli isolates from Arapirama gigas, a major fish species in the Brazilian Amazon rivers and fish farms, were characterized regarding their antimicrobial susceptibility, virulence, and genetic diversity. A total of forty (40) specimens of A. gigas, including 20 farmed and 20 wild fish, were included. Thirty-four quinolone-resistant E. coli isolates were phenotypically tested by broth microdilution, while resistance and virulence genes were detected by PCR. Molecular epidemiology and genetic relatedness were analyzed by MLST and PFGE typing. The majority of isolates were classified as MDR and detected harboring blaCTX-M, qnrA and qnrB genes. Enterotoxigenic E. coli pathotype (ETEC) isolates were presented in low prevalence among farmed animals. MLST and PFGE genotyping revealed a wide genetic background, including the detection of internationally spread clones. The obtained data point out A. gigas as a reservoir in Brazilian Amazon aquatic ecosystems and warns of the interference of AMR strains in wildlife and environmental matrices.