A simple method for human peripheral blood monocyte isolation
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/6232 |
Resumo: | Universidade de Brasília. Faculdade de Ciências Sociais. Departamento de Patologia. Brasília, DF, Brasil |
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Almeida, Marcos Cerqueira deSilva, Alan CBarral, Aldina Maria PradoBarral Netto, Manoel2013-01-22T21:12:43Z2013-01-22T21:12:43Z2000ALMEIDA, M. C. de et al. A simple method for human peripheral blood monocyte isolation. Memórias do Instituto Oswaldo Cruz, v. 95, n. 2, p. 221-223, mar.-apr. 2000.0074-0276https://www.arca.fiocruz.br/handle/icict/6232engA simple method for human peripheral blood monocyte isolationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Brasília. Faculdade de Ciências Sociais. Departamento de Patologia. Brasília, DF, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilWe describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.MonocytesPercollGradientIsolationSeparação Celular/métodosCentrifugação com Gradiente deConcentração/métodosMonócitosHumanosResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAlmeida, Marcos C. A simple method....pdfAlmeida, Marcos C. 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dc.title.pt_BR.fl_str_mv |
A simple method for human peripheral blood monocyte isolation |
title |
A simple method for human peripheral blood monocyte isolation |
spellingShingle |
A simple method for human peripheral blood monocyte isolation Almeida, Marcos Cerqueira de Monocytes Percoll Gradient Isolation Separação Celular/métodos Centrifugação com Gradiente de Concentração/métodos Monócitos Humanos Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. |
title_short |
A simple method for human peripheral blood monocyte isolation |
title_full |
A simple method for human peripheral blood monocyte isolation |
title_fullStr |
A simple method for human peripheral blood monocyte isolation |
title_full_unstemmed |
A simple method for human peripheral blood monocyte isolation |
title_sort |
A simple method for human peripheral blood monocyte isolation |
author |
Almeida, Marcos Cerqueira de |
author_facet |
Almeida, Marcos Cerqueira de Silva, Alan C Barral, Aldina Maria Prado Barral Netto, Manoel |
author_role |
author |
author2 |
Silva, Alan C Barral, Aldina Maria Prado Barral Netto, Manoel |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Almeida, Marcos Cerqueira de Silva, Alan C Barral, Aldina Maria Prado Barral Netto, Manoel |
dc.subject.en.pt_BR.fl_str_mv |
Monocytes Percoll Gradient Isolation |
topic |
Monocytes Percoll Gradient Isolation Separação Celular/métodos Centrifugação com Gradiente de Concentração/métodos Monócitos Humanos Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. |
dc.subject.decs.pt_BR.fl_str_mv |
Separação Celular/métodos Centrifugação com Gradiente de Concentração/métodos Monócitos Humanos Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. |
description |
Universidade de Brasília. Faculdade de Ciências Sociais. Departamento de Patologia. Brasília, DF, Brasil |
publishDate |
2000 |
dc.date.issued.fl_str_mv |
2000 |
dc.date.accessioned.fl_str_mv |
2013-01-22T21:12:43Z |
dc.date.available.fl_str_mv |
2013-01-22T21:12:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALMEIDA, M. C. de et al. A simple method for human peripheral blood monocyte isolation. Memórias do Instituto Oswaldo Cruz, v. 95, n. 2, p. 221-223, mar.-apr. 2000. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/6232 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
identifier_str_mv |
ALMEIDA, M. C. de et al. A simple method for human peripheral blood monocyte isolation. Memórias do Instituto Oswaldo Cruz, v. 95, n. 2, p. 221-223, mar.-apr. 2000. 0074-0276 |
url |
https://www.arca.fiocruz.br/handle/icict/6232 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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