A simple method for human peripheral blood monocyte Isolation

Detalhes bibliográficos
Autor(a) principal: Almeida, Marcos C de
Data de Publicação: 2000
Outros Autores: Silva, Alan C, Barral, Aldina, Barral Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/25587
https://dx.doi.org/10.1590/S0074-02762000000200014
Resumo: We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.
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spelling A simple method for human peripheral blood monocyte IsolationMonócitosPercollIsolamento gradienteWe describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.Em processamentoInstituto Oswaldo Cruz, Ministério da Saúde2017-12-07T04:32:47Z2017-12-07T04:32:47Z2000info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMem. Inst. Oswaldo Cruz,v.95,n.2,p.221-223,2000http://repositorio.unb.br/handle/10482/25587https://dx.doi.org/10.1590/S0074-02762000000200014Almeida, Marcos C deSilva, Alan CBarral, AldinaBarral Netto, Manoelinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T19:04:10Zoai:repositorio.unb.br:10482/25587Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:10Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv A simple method for human peripheral blood monocyte Isolation
title A simple method for human peripheral blood monocyte Isolation
spellingShingle A simple method for human peripheral blood monocyte Isolation
Almeida, Marcos C de
Monócitos
Percoll
Isolamento gradiente
title_short A simple method for human peripheral blood monocyte Isolation
title_full A simple method for human peripheral blood monocyte Isolation
title_fullStr A simple method for human peripheral blood monocyte Isolation
title_full_unstemmed A simple method for human peripheral blood monocyte Isolation
title_sort A simple method for human peripheral blood monocyte Isolation
author Almeida, Marcos C de
author_facet Almeida, Marcos C de
Silva, Alan C
Barral, Aldina
Barral Netto, Manoel
author_role author
author2 Silva, Alan C
Barral, Aldina
Barral Netto, Manoel
author2_role author
author
author
dc.contributor.author.fl_str_mv Almeida, Marcos C de
Silva, Alan C
Barral, Aldina
Barral Netto, Manoel
dc.subject.por.fl_str_mv Monócitos
Percoll
Isolamento gradiente
topic Monócitos
Percoll
Isolamento gradiente
description We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.
publishDate 2000
dc.date.none.fl_str_mv 2000
2017-12-07T04:32:47Z
2017-12-07T04:32:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Mem. Inst. Oswaldo Cruz,v.95,n.2,p.221-223,2000
http://repositorio.unb.br/handle/10482/25587
https://dx.doi.org/10.1590/S0074-02762000000200014
identifier_str_mv Mem. Inst. Oswaldo Cruz,v.95,n.2,p.221-223,2000
url http://repositorio.unb.br/handle/10482/25587
https://dx.doi.org/10.1590/S0074-02762000000200014
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
_version_ 1814508258192261120

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