Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction

Detalhes bibliográficos
Autor(a) principal: Queiroz, Fábio Ribeiro
Data de Publicação: 2017
Outros Autores: Silva, Luciana Maria, Jeremias, Wander de Jesus, Babá, Élio Hideo, Caldeira, Roberta Lima, Coelho, Paulo Marcos Zech, Gomes, Matheus de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25986
Resumo: Fundação Ezequiel Dias. Serviço de Biologia Celular do Departamento de Pesquisas e Desenvolvimento. Belo Horizonte, MG, Brasil.
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spelling Queiroz, Fábio RibeiroSilva, Luciana MariaJeremias, Wander de JesusBabá, Élio HideoCaldeira, Roberta LimaCoelho, Paulo Marcos ZechGomes, Matheus de Souza2018-04-20T16:49:56Z2018-04-20T16:49:56Z2017QUEIROZ, Fábio Ribeiro et al. Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction. PLoS One, v. 12, n. 7, p. 1-20, 2017.1932-6203https://www.arca.fiocruz.br/handle/icict/2598610.1371/journal.pone.0181483engPublic Library of ScienceroRNAsSchistosoma mansoniroRNAsSchistosoma mansoniGene expressionSmall interfering RNAsPhylogenetic analysisInvertebrate genomicsParasitic diseasesParasitic life cyclesDifferential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interactioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Ezequiel Dias. Serviço de Biologia Celular do Departamento de Pesquisas e Desenvolvimento. Belo Horizonte, MG, Brasil.Fundação Ezequiel Dias. Serviço de Biologia Celular do Departamento de Pesquisas e Desenvolvimento. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Biologia do Schistosoma mansoni e sua interação com hospedeiro. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisa em Biologia do Schistosoma mansoni e sua Interação com o Hospedeiro. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Biologia do Schistosoma mansoni e sua interação com hospedeiro. Belo Horizonte, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brasil.The World Health Organization (WHO) estimates that approximately 240 million people in 78 countries require treatment for schistosomiasis, an endemic disease caused by trematodes of the genus Schistosoma. In Brazil, Schistosoma mansoni is the only species representative of the genus whose passage through an invertebrate host, snails of the genus Biomphalaria, is obligatory before infecting a mammalian host, including humans. The availability of the genome and transcriptome of B. glabrata makes studying the regulation of gene expression, particularly the regulation of miRNA and piRNA processing pathway genes, possible. This might assist in better understanding the biology of B. glabrata as well as its relationship to the parasite S. mansoni. Some aspects of this interaction are still poorly explored, including the participation of non-coding small RNAs, such as miRNAs and piRNAs, with lengths varying from 18 to 30 nucleotides in mature form, which are potent regulators of gene expression. Using bioinformatics tools and quantitative PCR, we characterized and validated the miRNA and piRNA processing pathway genes in B. glabrata. In silico analyses showed that genes involved in miRNA and piRNA pathways were highly conserved in protein domain distribution, catalytic site residue conservation and phylogenetic analysis. Our study showed differential expression of putative Argonaute, Drosha, Piwi, Exportin-5 and Tudor genes at different snail developmental stages and during infection with S. mansoni, suggesting that the machinery is required for miRNA and piRNA processing in B. glabrata at all stages. These data suggested that the silencing pathway mediated by miRNAs and piRNAs can interfere in snail biology throughout the life cycle of the snail, thereby influencing the B. glabrata/S. mansoni interaction. Further studies are needed to confirm the participation of the small RNA processing pathway proteins in the parasite/host relationship, mainly the effective participation of small RNAs in regulating their target genes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/25986/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALDifferential expression of small RNA pathway genes associated with the Biomphalaria glabrata_Schistosoma mansoni interaction.pdfDifferential expression of small RNA pathway genes associated with the Biomphalaria glabrata_Schistosoma mansoni interaction.pdfapplication/pdf8714171https://www.arca.fiocruz.br/bitstream/icict/25986/2/Differential%20expression%20of%20small%20RNA%20pathway%20genes%20associated%20with%20the%20Biomphalaria%20glabrata_Schistosoma%20mansoni%20interaction.pdf6fa9ae3325e0a0aae95c142e8464d1bfMD52TEXTDifferential expression of small RNA pathway genes associated with the Biomphalaria glabrata_Schistosoma mansoni interaction.pdf.txtDifferential expression of small RNA pathway genes associated with the Biomphalaria glabrata_Schistosoma mansoni interaction.pdf.txtExtracted texttext/plain68722https://www.arca.fiocruz.br/bitstream/icict/25986/3/Differential%20expression%20of%20small%20RNA%20pathway%20genes%20associated%20with%20the%20Biomphalaria%20glabrata_Schistosoma%20mansoni%20interaction.pdf.txt537f0a034ddfe2b87bb60bec0afb18b6MD53icict/259862019-06-19 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dc.title.pt_BR.fl_str_mv Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction
title Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction
spellingShingle Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction
Queiroz, Fábio Ribeiro
roRNAs
Schistosoma mansoni
roRNAs
Schistosoma mansoni
Gene expression
Small interfering RNAs
Phylogenetic analysis
Invertebrate genomics
Parasitic diseases
Parasitic life cycles
title_short Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction
title_full Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction
title_fullStr Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction
title_full_unstemmed Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction
title_sort Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction
author Queiroz, Fábio Ribeiro
author_facet Queiroz, Fábio Ribeiro
Silva, Luciana Maria
Jeremias, Wander de Jesus
Babá, Élio Hideo
Caldeira, Roberta Lima
Coelho, Paulo Marcos Zech
Gomes, Matheus de Souza
author_role author
author2 Silva, Luciana Maria
Jeremias, Wander de Jesus
Babá, Élio Hideo
Caldeira, Roberta Lima
Coelho, Paulo Marcos Zech
Gomes, Matheus de Souza
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Queiroz, Fábio Ribeiro
Silva, Luciana Maria
Jeremias, Wander de Jesus
Babá, Élio Hideo
Caldeira, Roberta Lima
Coelho, Paulo Marcos Zech
Gomes, Matheus de Souza
dc.subject.other.pt_BR.fl_str_mv roRNAs
Schistosoma mansoni
topic roRNAs
Schistosoma mansoni
roRNAs
Schistosoma mansoni
Gene expression
Small interfering RNAs
Phylogenetic analysis
Invertebrate genomics
Parasitic diseases
Parasitic life cycles
dc.subject.en.pt_BR.fl_str_mv roRNAs
Schistosoma mansoni
Gene expression
Small interfering RNAs
Phylogenetic analysis
Invertebrate genomics
Parasitic diseases
Parasitic life cycles
description Fundação Ezequiel Dias. Serviço de Biologia Celular do Departamento de Pesquisas e Desenvolvimento. Belo Horizonte, MG, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-04-20T16:49:56Z
dc.date.available.fl_str_mv 2018-04-20T16:49:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv QUEIROZ, Fábio Ribeiro et al. Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction. PLoS One, v. 12, n. 7, p. 1-20, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25986
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0181483
identifier_str_mv QUEIROZ, Fábio Ribeiro et al. Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction. PLoS One, v. 12, n. 7, p. 1-20, 2017.
1932-6203
10.1371/journal.pone.0181483
url https://www.arca.fiocruz.br/handle/icict/25986
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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