Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16257 |
Resumo: | Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Nacional de Desenvolvimento Científico e Tecnol ogico (CNPq) |
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Siedler, Bianca SicaRoloff, Bárbara CoutoSá, Gizele L. deNeis, AlessandraConceição, Fabrício RochedoHartwig, Daiane DrawnzBorsuk, SibeleDellagostin, Odir AntonioCampos, Fabrício SRoehe, Paulo MichelHartleben, Claudia PMcBride, Alan John Alexander2016-10-17T14:15:45Z2016-10-17T14:15:45Z2016SIEDLER, B. S. et al. Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle. Protein Expression and Purification, v. 130, p. 21e27, 2016.1046-5928https://www.arca.fiocruz.br/handle/icict/1625710.1016/j.pep.2016.09.018Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Nacional de Desenvolvimento Científico e Tecnol ogico (CNPq)Universidade Federal de Pelotas. Núcleo de Biotecnologia - CDTec. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Núcleo de Biotecnologia - CDTec. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Núcleo de Biotecnologia - CDTec. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Núcleo de Biotecnologia - CDTec. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Núcleo de Biotecnologia - CDTec. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Instituto de Biologia. Departamento de Microbiologia e Parasitologia. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Núcleo de Biotecnologia - CDTec. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Núcleo de Biotecnologia - CDTec. Pelotas, RS, BrasilUniversidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas e da Saúde. Porto Alegre, RS, BrasilUniversidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas e da Saúde. Porto Alegre, RS, BrasilUniversidade Federal de Pelotas. Núcleo de Biotecnologia - CDTec. Pelotas, RS, BrasilUniversidade Federal de Pelotas. Núcleo de Biotecnologia - CDTec. Pelotas, RS, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilBovine herpesvirus (BoHV) glycoprotein E (gE) is a non-essential envelope glycoprotein and the deletion of gE has been used to develop BoHV-1 and BoHV-5 differential vaccine strains. The DIVA (Differentiation of Infected from Vaccinated Animals) strategy, using marker vaccines based on gE-negative BoHV strains, allows the identification of vaccinated or infected animals in immunoassays designed to detect anti-gE antibodies. In this study a codon optimized synthetic sequence of gE containing highly conserved regions from BoHV-1 and BoHV-5 was expressed in Pichia pastoris. Following expression, the recombinant gE (rgE) was secreted and purified from the culture medium. The rgE was identified by Western blotting (WB) using sera from cattle naturally infected with BoHV-1 and/or BoHV-5, or sera from bovines experimentally infected with wild-type BoHV-5. Sera collected from cattle vaccinated with a BoHV-5 gI/gE/US9¯ marker vaccine failed to recognise rgE. Expression of rgE, based on a sequence containing highly conserved regions from BoHV-1 and BoHV-5, in P. pastoris enabled the production of large quantities of rgE suitable for use in immunoassays for the differentiation vaccinated or infected cattle.engElsevierVacinaRinotraqueíte infecciosa bovinaVulvovaginite pustulosa infecciosaBalanopostite pustulosa infecciosaEncefalite herpética bovinaImunoensaioAnimaisImmunoassayDifferential vaccineInfectious bovine rhinotracheitisInfectious pustular vulvovaginitisInfectious pustular balanoposthitisBovine herpetic encephalitisSecretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle |
| title |
Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle |
| spellingShingle |
Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle Siedler, Bianca Sica Vacina Rinotraqueíte infecciosa bovina Vulvovaginite pustulosa infecciosa Balanopostite pustulosa infecciosa Encefalite herpética bovina Imunoensaio Animais Immunoassay Differential vaccine Infectious bovine rhinotracheitis Infectious pustular vulvovaginitis Infectious pustular balanoposthitis Bovine herpetic encephalitis |
| title_short |
Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle |
| title_full |
Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle |
| title_fullStr |
Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle |
| title_full_unstemmed |
Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle |
| title_sort |
Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle |
| author |
Siedler, Bianca Sica |
| author_facet |
Siedler, Bianca Sica Roloff, Bárbara Couto Sá, Gizele L. de Neis, Alessandra Conceição, Fabrício Rochedo Hartwig, Daiane Drawnz Borsuk, Sibele Dellagostin, Odir Antonio Campos, Fabrício S Roehe, Paulo Michel Hartleben, Claudia P McBride, Alan John Alexander |
| author_role |
author |
| author2 |
Roloff, Bárbara Couto Sá, Gizele L. de Neis, Alessandra Conceição, Fabrício Rochedo Hartwig, Daiane Drawnz Borsuk, Sibele Dellagostin, Odir Antonio Campos, Fabrício S Roehe, Paulo Michel Hartleben, Claudia P McBride, Alan John Alexander |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Siedler, Bianca Sica Roloff, Bárbara Couto Sá, Gizele L. de Neis, Alessandra Conceição, Fabrício Rochedo Hartwig, Daiane Drawnz Borsuk, Sibele Dellagostin, Odir Antonio Campos, Fabrício S Roehe, Paulo Michel Hartleben, Claudia P McBride, Alan John Alexander |
| dc.subject.other.pt_BR.fl_str_mv |
Vacina Rinotraqueíte infecciosa bovina Vulvovaginite pustulosa infecciosa Balanopostite pustulosa infecciosa Encefalite herpética bovina Imunoensaio Animais |
| topic |
Vacina Rinotraqueíte infecciosa bovina Vulvovaginite pustulosa infecciosa Balanopostite pustulosa infecciosa Encefalite herpética bovina Imunoensaio Animais Immunoassay Differential vaccine Infectious bovine rhinotracheitis Infectious pustular vulvovaginitis Infectious pustular balanoposthitis Bovine herpetic encephalitis |
| dc.subject.en.pt_BR.fl_str_mv |
Immunoassay Differential vaccine Infectious bovine rhinotracheitis Infectious pustular vulvovaginitis Infectious pustular balanoposthitis Bovine herpetic encephalitis |
| description |
Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Nacional de Desenvolvimento Científico e Tecnol ogico (CNPq) |
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2016 |
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2016-10-17T14:15:45Z |
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2016-10-17T14:15:45Z |
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2016 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SIEDLER, B. S. et al. Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle. Protein Expression and Purification, v. 130, p. 21e27, 2016. |
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https://www.arca.fiocruz.br/handle/icict/16257 |
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1046-5928 |
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10.1016/j.pep.2016.09.018 |
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SIEDLER, B. S. et al. Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle. Protein Expression and Purification, v. 130, p. 21e27, 2016. 1046-5928 10.1016/j.pep.2016.09.018 |
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Elsevier |
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Elsevier |
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