Mitichrondial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection

Detalhes bibliográficos
Autor(a) principal: Stumbo, Ana Carolina
Data de Publicação: 2008
Outros Autores: Cortez, Erika, Rodrigues, Carlos Alberto, Henriques, Maria das Graças M. O., Porto, Luís Cristóvão, Barbosa, Helene S., Carvalho, Laís
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32432
Resumo: Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.
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spelling Stumbo, Ana CarolinaCortez, ErikaRodrigues, Carlos AlbertoHenriques, Maria das Graças M. O.Porto, Luís CristóvãoBarbosa, Helene S.Carvalho, Laís2019-04-10T13:58:43Z2019-04-10T13:58:43Z2008STUMBO, Ana Carolina et al. Mitochondrial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection. Cell Biology International, v. 32, p. 235-238, 2008.1065-6995https://www.arca.fiocruz.br/handle/icict/3243210.1016/j.cellbi.2007.08.031engElsevierToxoplasma gondiiCélula endotelial humanaMitocôndriasProteína HMGB1Human endothelial cellMitochondriaHMGB1Toxoplasma gondiiMitichrondial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Instituto de Biologia. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.Toxoplasma gondii is an obligate intracellular pathogen, replicating only within a specialized membrane-bounded cytoplasmic vacuole, the parasitophorous vacuole (PV), which interacts with host cell mitochondria. High mobility group box 1 (HMGB1), a known nuclear transcription factor, also may be involved in pathological conditions, whose function is to signal tissue damage. Using confocal microscopy, we have investigated the localization of HMGB1 and the mitochondria performance during interaction between human umbilical vein endothelial cells (HUVEC) and Toxoplasma. Immunofluorescence showed HMGB1 localization in HUVEC tubular mitochondria stained with Mito Tracker (MT). At 2 h post-infection, MT labeled spherical structures scattered throughout the cytoplasm and HMGB1 were still present. After 24 h of infection, long and tubular structures were localized around PVs and were double labeled by MT and HMGB1, suggesting a structural reorganization of the mitochondria over a long period of infection. For the first time, these results show there is HMGB1 in HUVEC mitochondria and that this protein could be playing a part in mitochondrial DNA events which are important for fission and fusion processes reported here during HUVEC-T. gondii infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32432/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALHeleneS_BarbosaLPorto_etal_IOC_2008.pdfHeleneS_BarbosaLPorto_etal_IOC_2008.pdfapplication/pdf562357https://www.arca.fiocruz.br/bitstream/icict/32432/2/HeleneS_BarbosaLPorto_etal_IOC_2008.pdfc7eff3721586cf8e27dd6c1c398f5b87MD52TEXTHeleneS_BarbosaLPorto_etal_IOC_2008.pdf.txtHeleneS_BarbosaLPorto_etal_IOC_2008.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Mitichrondial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection
title Mitichrondial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection
spellingShingle Mitichrondial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection
Stumbo, Ana Carolina
Toxoplasma gondii
Célula endotelial humana
Mitocôndrias
Proteína HMGB1
Human endothelial cell
Mitochondria
HMGB1
Toxoplasma gondii
title_short Mitichrondial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection
title_full Mitichrondial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection
title_fullStr Mitichrondial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection
title_full_unstemmed Mitichrondial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection
title_sort Mitichrondial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection
author Stumbo, Ana Carolina
author_facet Stumbo, Ana Carolina
Cortez, Erika
Rodrigues, Carlos Alberto
Henriques, Maria das Graças M. O.
Porto, Luís Cristóvão
Barbosa, Helene S.
Carvalho, Laís
author_role author
author2 Cortez, Erika
Rodrigues, Carlos Alberto
Henriques, Maria das Graças M. O.
Porto, Luís Cristóvão
Barbosa, Helene S.
Carvalho, Laís
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Stumbo, Ana Carolina
Cortez, Erika
Rodrigues, Carlos Alberto
Henriques, Maria das Graças M. O.
Porto, Luís Cristóvão
Barbosa, Helene S.
Carvalho, Laís
dc.subject.other.pt_BR.fl_str_mv Toxoplasma gondii
Célula endotelial humana
Mitocôndrias
Proteína HMGB1
topic Toxoplasma gondii
Célula endotelial humana
Mitocôndrias
Proteína HMGB1
Human endothelial cell
Mitochondria
HMGB1
Toxoplasma gondii
dc.subject.en.pt_BR.fl_str_mv Human endothelial cell
Mitochondria
HMGB1
Toxoplasma gondii
description Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2019-04-10T13:58:43Z
dc.date.available.fl_str_mv 2019-04-10T13:58:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv STUMBO, Ana Carolina et al. Mitochondrial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection. Cell Biology International, v. 32, p. 235-238, 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32432
dc.identifier.issn.pt_BR.fl_str_mv 1065-6995
dc.identifier.doi.none.fl_str_mv 10.1016/j.cellbi.2007.08.031
identifier_str_mv STUMBO, Ana Carolina et al. Mitochondrial localization of non-histone protein HMGB1 during human endothelial cell - Toxoplasma gondii infection. Cell Biology International, v. 32, p. 235-238, 2008.
1065-6995
10.1016/j.cellbi.2007.08.031
url https://www.arca.fiocruz.br/handle/icict/32432
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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