Na/K-ATPase assay in the intact mice lung subjected to perfusion
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10544 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
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Albuquerque, Cassiano Felippe Gonçalves deBurth, PatríciaSilva, Adriana RibeiroMoraes, Isabel Matos Medeiros deOliveira, Flora Magno de JesusSantelli, Ricardo ErthalFreire, Aline SoaresIbrahim, Mauricio YounesFaria Neto, Hugo Caire de CastroFaria, Mauro Velho de Castro2015-05-27T13:39:50Z2015-05-27T13:39:50Z2014ALBUQUERQUE, Cassiano Felippe G. et al. Na/K-ATPase assay in the intact mice lung subjected to perfusion. BMC Research Notes, v.7:798, 6p, 2014.https://www.arca.fiocruz.br/handle/icict/1054410.1186/1756-0500-7-798engBioMed CentralPulmãoLungNa/K-ATPaseInductively coupled plasma optical emission spectrometerAcute respiratory distress syndrome (ARDS)pneumocyte Na/K-ATPaseNa/K-ATPase assay in the intact mice lung subjected to perfusioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Bio-Manguinhos. Departamento de Reativos para Diagnóstico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Bio-Manguinhos. Departamento de Reativos para Diagnóstico. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Química Analítica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Química Analítica. Rio de Janeiro, RJ, Brasil.Among the characteristics of acute respiratory distress syndrome (ARDS) is edema formation and its resolution depends on pneumocyte Na/K-ATPase activity. Increased concentration of oleic acid (OA) in plasma induces lung injury by targeting Na/K-ATPase and, thus, interfering in sodium transport. FINDINGS: Presently, we adapted a radioactivity-free assay to detect Na/K-ATPase activity in perfused lung mice, comparing the inhibitory effect of ouabain and OA. We managed to perfuse only the lung, avoiding the systemic loss of rubidium. Rb+ incorporation into lung was measured by inductively coupled plasma optical emission spectrometry (ICP OES) technique, after lung tissue digestion. Na/K-ATPase activity was the difference between Rb+ incorporation with or without ouabain. Lung Na/K-ATPase was completely inhibited by perfusion with ouabain. However, OA caused a partial inhibition. CONCLUSIONS: In the present work the amount of incorporated Rb+ was greater than seen in our previous report, showing that the present technique is trustworthy. This new proposed assay may allow researchers to study the importance of Na/K-ATPase activity in lung pathophysiology.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10544/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALadriana_silvaetal_IOC_2014.pdfapplication/pdf350633https://www.arca.fiocruz.br/bitstream/icict/10544/2/adriana_silvaetal_IOC_2014.pdf5135d467f38c8b0fb3cbe9daca32f22bMD52TEXTadriana_silvaetal_IOC_2014.pdf.txtadriana_silvaetal_IOC_2014.pdf.txtExtracted texttext/plain26300https://www.arca.fiocruz.br/bitstream/icict/10544/3/adriana_silvaetal_IOC_2014.pdf.txt6a538758536892a94bc75765be83be3dMD53icict/105442022-06-24 12:17:31.845oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:31Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Na/K-ATPase assay in the intact mice lung subjected to perfusion |
| title |
Na/K-ATPase assay in the intact mice lung subjected to perfusion |
| spellingShingle |
Na/K-ATPase assay in the intact mice lung subjected to perfusion Albuquerque, Cassiano Felippe Gonçalves de Pulmão Lung Na/K-ATPase Inductively coupled plasma optical emission spectrometer Acute respiratory distress syndrome (ARDS) pneumocyte Na/K-ATPase |
| title_short |
Na/K-ATPase assay in the intact mice lung subjected to perfusion |
| title_full |
Na/K-ATPase assay in the intact mice lung subjected to perfusion |
| title_fullStr |
Na/K-ATPase assay in the intact mice lung subjected to perfusion |
| title_full_unstemmed |
Na/K-ATPase assay in the intact mice lung subjected to perfusion |
| title_sort |
Na/K-ATPase assay in the intact mice lung subjected to perfusion |
| author |
Albuquerque, Cassiano Felippe Gonçalves de |
| author_facet |
Albuquerque, Cassiano Felippe Gonçalves de Burth, Patrícia Silva, Adriana Ribeiro Moraes, Isabel Matos Medeiros de Oliveira, Flora Magno de Jesus Santelli, Ricardo Erthal Freire, Aline Soares Ibrahim, Mauricio Younes Faria Neto, Hugo Caire de Castro Faria, Mauro Velho de Castro |
| author_role |
author |
| author2 |
Burth, Patrícia Silva, Adriana Ribeiro Moraes, Isabel Matos Medeiros de Oliveira, Flora Magno de Jesus Santelli, Ricardo Erthal Freire, Aline Soares Ibrahim, Mauricio Younes Faria Neto, Hugo Caire de Castro Faria, Mauro Velho de Castro |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Albuquerque, Cassiano Felippe Gonçalves de Burth, Patrícia Silva, Adriana Ribeiro Moraes, Isabel Matos Medeiros de Oliveira, Flora Magno de Jesus Santelli, Ricardo Erthal Freire, Aline Soares Ibrahim, Mauricio Younes Faria Neto, Hugo Caire de Castro Faria, Mauro Velho de Castro |
| dc.subject.other.pt_BR.fl_str_mv |
Pulmão |
| topic |
Pulmão Lung Na/K-ATPase Inductively coupled plasma optical emission spectrometer Acute respiratory distress syndrome (ARDS) pneumocyte Na/K-ATPase |
| dc.subject.en.pt_BR.fl_str_mv |
Lung Na/K-ATPase Inductively coupled plasma optical emission spectrometer Acute respiratory distress syndrome (ARDS) pneumocyte Na/K-ATPase |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2014 |
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2014 |
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2015-05-27T13:39:50Z |
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2015-05-27T13:39:50Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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ALBUQUERQUE, Cassiano Felippe G. et al. Na/K-ATPase assay in the intact mice lung subjected to perfusion. BMC Research Notes, v.7:798, 6p, 2014. |
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https://www.arca.fiocruz.br/handle/icict/10544 |
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10.1186/1756-0500-7-798 |
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ALBUQUERQUE, Cassiano Felippe G. et al. Na/K-ATPase assay in the intact mice lung subjected to perfusion. BMC Research Notes, v.7:798, 6p, 2014. 10.1186/1756-0500-7-798 |
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BioMed Central |
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BioMed Central |
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