Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production

Detalhes bibliográficos
Autor(a) principal: Albuquerque, Cassiano Felippe Gonçalves de
Data de Publicação: 2013
Outros Autores: Burth, Patrícia, Silva, Adriana Ribeiro, Moraes, Isabel Matos Medeiros de, Oliveira, Flora Magno de Jesus, Santelli, Ricardo Erthal, Freire, Aline Soares, Bozza, Patrícia Torres, Younes-Ibrahim, Mauricio, Faria Neto, Hugo Caire de Castro, Faria, Mauro Velho de Castro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12615
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.
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spelling Albuquerque, Cassiano Felippe Gonçalves deBurth, PatríciaSilva, Adriana RibeiroMoraes, Isabel Matos Medeiros deOliveira, Flora Magno de JesusSantelli, Ricardo ErthalFreire, Aline SoaresBozza, Patrícia TorresYounes-Ibrahim, MauricioFaria Neto, Hugo Caire de CastroFaria, Mauro Velho de Castro2016-01-26T12:03:05Z2016-01-26T12:03:05Z2013ALBUQUERQUE, Cassiano Felippe Gonçalves de; et al. Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production. Journal of Inflammation, v.10, n.34, 10p, 2013.https://www.arca.fiocruz.br/handle/icict/1261510.1186/1476-9255-10-341476-9255engBioMed CentralOleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid productioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense.Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Química Analítica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Química Analítica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Mèdicas. Departamento de Medicina Interna. Rio de Janeiro, RJ, Brasil.Background: Acute respiratory distress syndrome (ARDS) can emerge from certain pathologies, such as sepsis, fat embolism and leptospirosis, in which the levels of unesterified fatty acids are increased in the patient’s plasma. ARDS is characterized by edema formation, and edema resolution occurs mainly due to the pneumocyte Na/K-ATPase activity. As previously described, increased oleic acid (OA) plasma concentrations induce lung injury by interfering with sodium transport. The first aim of this study was to develop a radioactivity-free assay to detect Na,K-ATPase activity ex vivo using a model of OA-induced lung injury in mice. We also investigated the relationship between Na/K-ATPase inhibition and OA-induced lung injury using ouabain-induced lung injury as a comparison, because of the well-described effect of ouabain as a Na/K-ATPase inhibitor. Methods: We developed a Na/K-ATPase assay based on the capture of non-radioactive Rb+ ions by mice lung tissue in the absence or presence of ouabain, a specific Na/K-ATPase inhibitor. Rb+ incorporation into the lung was measured by inductively coupled plasma-optical emission spectrometry (ICP-OES) after lung tissue mineralization. Na/K-ATPase activity was considered as the difference between Rb+ incorporation in the absence and in the presence of ouabain. Bronchoalveolar lavage fluid was collected for lung injury assessment. For this assessment, cell counting, lipid body enumeration and lipid mediator concentrations were measured. Histological analyses were used to determinate lung pathology. Whole body plethysmographic analysis was performed to assay lung function. Results: The lung Na/K-ATPase activity of mice was completely inhibited by an OA dose of 10 μmol, an effect also obtained with 10-3 μmol of ouabain, as demonstrated by the decreased Rb+ incorporation in the lungs. The same OA dose induced lung edema and inflammation with cell influx, lipid body formation, and leukotriene B4 (LTB4) and prostaglandin E2 (PGE2) production. Ouabain also induced lung inflammation, as detected by histological examinations. As far as we know, this is the first time that ouabain-induced lung injury was shown. Both OA and ouabain induced functional lung pathology in mice simultaneously with inhibition of the lung Na/K-ATPase activity. Conclusions: We developed a new non-radioactive assay to quantified Na/K-ATPase in vivo. OA and ouabain inhibited in vivo Na/K-ATPase activity in the lungs and induced lung injury. Our data reinforce the idea that Na/K-ATPase inhibitors may worsen lung injury in specific pathological conditions.Oleic acidNa/K-ATPaseLungLipid bodyPGELTBÁcido OleicoProstaglandinas EPulmãoEicosanoidesLeucócitosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12615/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALcassiano_albuquerque_etal_IOC_2013.pdfcassiano_albuquerque_etal_IOC_2013.pdfapplication/pdf2143360https://www.arca.fiocruz.br/bitstream/icict/12615/2/cassiano_albuquerque_etal_IOC_2013.pdf8f5e5674d7c469c0ea089e8c27de22edMD52TEXTcassiano_albuquerque_etal_IOC_2013.pdf.txtcassiano_albuquerque_etal_IOC_2013.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production
title Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production
spellingShingle Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production
Albuquerque, Cassiano Felippe Gonçalves de
Oleic acid
Na/K-ATPase
Lung
Lipid body
PGE
LTB
Ácido Oleico
Prostaglandinas E
Pulmão
Eicosanoides
Leucócitos
title_short Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production
title_full Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production
title_fullStr Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production
title_full_unstemmed Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production
title_sort Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production
author Albuquerque, Cassiano Felippe Gonçalves de
author_facet Albuquerque, Cassiano Felippe Gonçalves de
Burth, Patrícia
Silva, Adriana Ribeiro
Moraes, Isabel Matos Medeiros de
Oliveira, Flora Magno de Jesus
Santelli, Ricardo Erthal
Freire, Aline Soares
Bozza, Patrícia Torres
Younes-Ibrahim, Mauricio
Faria Neto, Hugo Caire de Castro
Faria, Mauro Velho de Castro
author_role author
author2 Burth, Patrícia
Silva, Adriana Ribeiro
Moraes, Isabel Matos Medeiros de
Oliveira, Flora Magno de Jesus
Santelli, Ricardo Erthal
Freire, Aline Soares
Bozza, Patrícia Torres
Younes-Ibrahim, Mauricio
Faria Neto, Hugo Caire de Castro
Faria, Mauro Velho de Castro
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Albuquerque, Cassiano Felippe Gonçalves de
Burth, Patrícia
Silva, Adriana Ribeiro
Moraes, Isabel Matos Medeiros de
Oliveira, Flora Magno de Jesus
Santelli, Ricardo Erthal
Freire, Aline Soares
Bozza, Patrícia Torres
Younes-Ibrahim, Mauricio
Faria Neto, Hugo Caire de Castro
Faria, Mauro Velho de Castro
dc.subject.en.pt_BR.fl_str_mv Oleic acid
Na/K-ATPase
Lung
Lipid body
PGE
LTB
topic Oleic acid
Na/K-ATPase
Lung
Lipid body
PGE
LTB
Ácido Oleico
Prostaglandinas E
Pulmão
Eicosanoides
Leucócitos
dc.subject.decs.pt_BR.fl_str_mv Ácido Oleico
Prostaglandinas E
Pulmão
Eicosanoides
Leucócitos
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-01-26T12:03:05Z
dc.date.available.fl_str_mv 2016-01-26T12:03:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALBUQUERQUE, Cassiano Felippe Gonçalves de; et al. Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production. Journal of Inflammation, v.10, n.34, 10p, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12615
dc.identifier.doi.none.fl_str_mv 10.1186/1476-9255-10-34
dc.identifier.eissn.none.fl_str_mv 1476-9255
identifier_str_mv ALBUQUERQUE, Cassiano Felippe Gonçalves de; et al. Oleic acid inhibits lung Na/K-ATPase in mice and induces injury with lipid body formation in leukocytes and eicosanoid production. Journal of Inflammation, v.10, n.34, 10p, 2013.
10.1186/1476-9255-10-34
1476-9255
url https://www.arca.fiocruz.br/handle/icict/12615
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/12615/2/cassiano_albuquerque_etal_IOC_2013.pdf
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