Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44157 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia; Rio de Janeiro, RJ, Brasil. |
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Queiroz, Adriana O.Legey, Ana P.Xavier, Samanta C. C.Jansen, Ana M.2020-10-23T20:39:44Z2020-10-23T20:39:44Z2001QUEIROZ, Adriana O. et al. Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 7, p. 965-972, Oct. 2001.0074-0276https://www.arca.fiocruz.br/handle/icict/4415710.1590/S0074-027620010007000141678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Trypanosoma evansiRatos WistarReconhecimento antigênicoTeste indireto de anticorpos fluorescentesTrypanosoma evansiWistar ratsAntigenic recognitionIndirect fluorescent antibody testSpecific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia; Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia; Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia; Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia; Rio de Janeiro, RJ, Brasil.“Mal de Cadeiras”, an enzootic disease caused by Trypanosoma evansi, is one of the most important trypanosomiases in the Brazilian Pantanal region. The disease affects mainly horses, which are widely used in extensive cattle production, an activity of greatest economical significance for the region. The parasite also infects sylvan (coatis and capybaras) and domestic (dogs) animals, respectively considered wild and domestic reservoirs of T. evansi. For a better understanding of the interaction of T. evansi with its rodent host, we evaluated the differences in the specific antibody level patterns and in the parasitic peptides recognition patterns of experimentally infected Wistar rats. The rats experimentally infected with T. evansi isolates obtained from coatis, dogs and horses were submitted to indirect immunofluorescence test (IgM e IgG) and Western blotting. The serological titers for IgM and IgG ranged between 1:40 and 1:160. The most recognized polypeptide profiles were in a range of 17 and 74 kDa. Our data suggest that the humoral immune response in Wistar rats is not sufficient for granting an effective control of T. evansi infections.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44157/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAdrianaQueiroz_SamantaXavier_etal_IOC_2001.pdfAdrianaQueiroz_SamantaXavier_etal_IOC_2001.pdfapplication/pdf149403https://www.arca.fiocruz.br/bitstream/icict/44157/2/AdrianaQueiroz_SamantaXavier_etal_IOC_2001.pdf086f8540777ffd1794bd2448ebf014aaMD52TEXTAdrianaQueiroz_SamantaXavier_etal_IOC_2001.pdf.txtAdrianaQueiroz_SamantaXavier_etal_IOC_2001.pdf.txtExtracted texttext/plain29796https://www.arca.fiocruz.br/bitstream/icict/44157/3/AdrianaQueiroz_SamantaXavier_etal_IOC_2001.pdf.txtdacb42b2dacd3a7a8bf8d366600ab64fMD53icict/441572020-10-24 02:07:00.176oai:www.arca.fiocruz.br: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ório 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dc.title.pt_BR.fl_str_mv |
Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi |
title |
Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi |
spellingShingle |
Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi Queiroz, Adriana O. Trypanosoma evansi Ratos Wistar Reconhecimento antigênico Teste indireto de anticorpos fluorescentes Trypanosoma evansi Wistar rats Antigenic recognition Indirect fluorescent antibody test |
title_short |
Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi |
title_full |
Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi |
title_fullStr |
Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi |
title_full_unstemmed |
Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi |
title_sort |
Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi |
author |
Queiroz, Adriana O. |
author_facet |
Queiroz, Adriana O. Legey, Ana P. Xavier, Samanta C. C. Jansen, Ana M. |
author_role |
author |
author2 |
Legey, Ana P. Xavier, Samanta C. C. Jansen, Ana M. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Queiroz, Adriana O. Legey, Ana P. Xavier, Samanta C. C. Jansen, Ana M. |
dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma evansi Ratos Wistar Reconhecimento antigênico Teste indireto de anticorpos fluorescentes |
topic |
Trypanosoma evansi Ratos Wistar Reconhecimento antigênico Teste indireto de anticorpos fluorescentes Trypanosoma evansi Wistar rats Antigenic recognition Indirect fluorescent antibody test |
dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma evansi Wistar rats Antigenic recognition Indirect fluorescent antibody test |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia; Rio de Janeiro, RJ, Brasil. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001 |
dc.date.accessioned.fl_str_mv |
2020-10-23T20:39:44Z |
dc.date.available.fl_str_mv |
2020-10-23T20:39:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
QUEIROZ, Adriana O. et al. Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 7, p. 965-972, Oct. 2001. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/44157 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762001000700014 |
dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
identifier_str_mv |
QUEIROZ, Adriana O. et al. Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 7, p. 965-972, Oct. 2001. 0074-0276 10.1590/S0074-02762001000700014 1678-8060 |
url |
https://www.arca.fiocruz.br/handle/icict/44157 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
collection |
Repositório Institucional da FIOCRUZ (ARCA) |
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