Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection

Detalhes bibliográficos
Autor(a) principal: Martins, Sharon de Toledo
Data de Publicação: 2018
Outros Autores: Kuczera, Diogo, Lötvall, Jan, Bordignon, Juliano, Alves, Lysangela Ronalte
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30014
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
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spelling Martins, Sharon de ToledoKuczera, DiogoLötvall, JanBordignon, JulianoAlves, Lysangela Ronalte2018-11-13T15:30:12Z2018-11-13T15:30:12Z2018MARTINS, Sharon de T. et al. Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection. Frontiers in Microbiology, v. 9, n. 1792, p. 1-19, 2018.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/3001410.3389/fmicb.2018.017921664-302XporFrontiers MediaCharacterization of dendritic cell-derived extracellular vesicles during dengue virus infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Krefting Research Centre. University of Gothenburg, Gothenburg, Sweden.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.The dengue virus (DENV), transmitted by Aedes spp. mosquitoes, is one of the most important arboviral infections in the world. Dengue begins as a febrile condition, and in certain patients, it can evolve severe clinical outcomes, such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The reasons why certain patients develop DHF or DSS have not been thoroughly elucidated to date, and both patient and viral factors have been implicated. Previous work has shown that a severe immune dysfunction involving dendritic cells and T cells plays a key role in increasing the disease severity, especially in secondary heterologous infections. Extracellular vesicles (EVs) are membranous particles that are secreted by several cell types involved in homeostatic and pathological processes. Secretion of EVs by infected cells can enhance immune responses or favor viral evasion. In this study, we compare the molecular content of EVs that are secreted by human primary dendritic cells under different conditions: uninfected or infected with DENV3 strains isolated from patients with different infection phenotypes (a severe case involving DSS and a mild case). Human monocyte-derived dendritic cells (mdDCs) were infected with the dengue virus strains DENV3 5532 (severe) or DENV3 290 (mild), and the EVs were isolated. The presence of cup-shaped EVs was confirmed by electron microscopy and immunostaining with CD9, CD81, and CD83. The RNA content from the mdDC-infected cells contained several mRNAs and miRNAs related to immune responses compared to the EVs from mock-infected mdDCs. A number of these RNAs were detected exclusively during infection with DENV3 290 or DENV3 5532. This result suggests that the differential immune modulation of mdDCs by dengue strains can be achieved through the EV pathway. Additionally, we observed an association of EVs with DENV-infectious particles that seem to be protected from antibodies targeting the DENV envelope protein. We also showed that EVs derived from cells treated with IFN alpha have a protective effect against DENV infection in other cells. These results suggested that during DENV infection, the EV pathway could be exploited to favor viral viability, although immune mechanisms to counteract viral infection can also involve DC-derived EVs.Extracellular VesiclesInfectionNeglected DiseasesInfecciónEnfermedades DesatendidasVesículas ExtracelularesDengueInfecçãoDoenças Negligenciadasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/30014/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALfmicb-09-017.pdffmicb-09-017.pdfapplication/pdf6158033https://www.arca.fiocruz.br/bitstream/icict/30014/2/fmicb-09-017.pdf81b081bdc3768d3be0a5ca51a4c69984MD52TEXTfmicb-09-017.pdf.txtfmicb-09-017.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection
title Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection
spellingShingle Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection
Martins, Sharon de Toledo
Extracellular Vesicles
Infection
Neglected Diseases
Infección
Enfermedades Desatendidas
Vesículas Extracelulares
Dengue
Infecção
Doenças Negligenciadas
title_short Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection
title_full Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection
title_fullStr Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection
title_full_unstemmed Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection
title_sort Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection
author Martins, Sharon de Toledo
author_facet Martins, Sharon de Toledo
Kuczera, Diogo
Lötvall, Jan
Bordignon, Juliano
Alves, Lysangela Ronalte
author_role author
author2 Kuczera, Diogo
Lötvall, Jan
Bordignon, Juliano
Alves, Lysangela Ronalte
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Martins, Sharon de Toledo
Kuczera, Diogo
Lötvall, Jan
Bordignon, Juliano
Alves, Lysangela Ronalte
dc.subject.en.pt_BR.fl_str_mv Extracellular Vesicles
Infection
Neglected Diseases
topic Extracellular Vesicles
Infection
Neglected Diseases
Infección
Enfermedades Desatendidas
Vesículas Extracelulares
Dengue
Infecção
Doenças Negligenciadas
dc.subject.es.pt_BR.fl_str_mv Infección
Enfermedades Desatendidas
dc.subject.decs.pt_BR.fl_str_mv Vesículas Extracelulares
Dengue
Infecção
Doenças Negligenciadas
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-11-13T15:30:12Z
dc.date.available.fl_str_mv 2018-11-13T15:30:12Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINS, Sharon de T. et al. Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection. Frontiers in Microbiology, v. 9, n. 1792, p. 1-19, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30014
dc.identifier.issn.pt_BR.fl_str_mv 1664-302X
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2018.01792
dc.identifier.eissn.none.fl_str_mv 1664-302X
identifier_str_mv MARTINS, Sharon de T. et al. Characterization of dendritic cell-derived extracellular vesicles during dengue virus infection. Frontiers in Microbiology, v. 9, n. 1792, p. 1-19, 2018.
1664-302X
10.3389/fmicb.2018.01792
url https://www.arca.fiocruz.br/handle/icict/30014
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/30014/1/license.txt
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