Cryptococcus extracellular vesicles properties and their use as vaccine platforms
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/49826 |
Resumo: | Institut Pasteur. Département de Mycologie. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. |
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Rizzo, JulianaWong, Sarah SzeWahGazi, Anastasia D.Moyrand, FrédériqueChaze, ThibaultCommere, Pierre-HenriNovault, SophieMatondo, MariettePéhau-Arnaudet, GérardReis, Flavia Coelho Garcia dosVos, MatthijnAlves, Lysangela RonalteMay, Robin C.Nimrichter, LeonardoRodrigues, Marcio LourençoAimanianda, VishukumarJanbon, Guilhem2021-11-12T19:13:45Z2021-11-12T19:13:45Z2021RIZZO, Juliana. et al. Cryptococcus extracellular vesicles properties and their use as vaccine platforms. Journal of Extracellular Vesicles, p. 1–19, 2021.2001-3078https://www.arca.fiocruz.br/handle/icict/4982610.1002/jev2.12129porInternational Society for Extracellular VesiclesCryptococcusInfecções por FungosCryptococcosisExtracellular VesiclesMycosesFungal InfectionsCriptococosisVesículas ExtracelularesMicosisInfecciones por HongosCryptococcusVésicules extracellulairesMycosesInfections fongiquesInfecção por Cryptococcus neoformansVesículas ExtracelularesMicosesCryptococcus extracellular vesicles properties and their use as vaccine platformsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstitut Pasteur. Département de Mycologie. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France.Institut Pasteur. Département de Mycologie. Unité Mycologie Moléculaire. Paris, France.Institut Pasteur. Département de Biologie Cellulaire et Infection. Ultrastructural Bio-Imaging UTechS UBI. Paris, France.Institut Pasteur. Département de Mycologie. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France.Institut Pasteur. Centre de Ressources et Recherches Technologiques. Plateforme Protéomique, Unité de Spectrométrie de Masse pour la Biologie. Paris, France.Institut Pasteur. Centre de Ressources et Recherches Technologiques. Cytometry and Biomarkers. Paris, France.Institut Pasteur. Centre de Ressources et Recherches Technologiques. Cytometry and Biomarkers. Paris, France.Institut Pasteur. Centre de Ressources et Recherches Technologiques. Plateforme Protéomique, Unité de Spectrométrie de Masse pour la Biologie. Paris, France.Institut Pasteur. Département de Biologie Cellulaire et Infection. Ultrastructural Bio-Imaging UTechS UBI. Paris, France.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Centre de Ressources et Recherches Technologiques. NanoImaging Core Facility. Paris, France.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.University of Birmingham. Institute of Microbiology and Infection and School of Biosciences. Birmingham, United Kingdom.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Département de Mycologie. Unité Mycologie Moléculaire. Paris, France.Institut Pasteur. Département de Mycologie. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France.Whereas extracellular vesicle (EV) research has become commonplace in different biomedical fields, this field of research is still in its infancy in mycology. Here we provide a robust set of data regarding the structural and compositional aspects of EVs isolated from the fungal pathogenic species Cryptococcus neoformans, C. deneoformans and C. deuterogattii. Using cutting-edge methodological approaches including cryogenic electron microscopy and cryogenic electron tomography, proteomics, and flow cytometry, we revisited cryptococcal EV features and suggest a new EV structural model, in which the vesicular lipid bilayer is covered by mannoprotein-based fibrillar decoration, bearing the capsule polysaccharide as its outer layer. About 10% of the EV population is devoid of fibrillar decoration, adding another aspect to EV diversity. By analysing EV protein cargo from the three species, we characterized the typical Cryptococcus EV proteome. It contains several membrane-bound protein families, including some Tsh proteins bearing a SUR7/PalI motif. The presence of known protective antigens on the surface of Cryptococcus EVs, resembling the morphology of encapsulated virus structures, suggested their potential as a vaccine. Indeed, mice immunized with EVs obtained from an acapsular C. neoformans mutant strain rendered a strong antibody response in mice and significantly prolonged their survival upon C. neoformans infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/49826/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALJEV2-10-e12129ok.pdfJEV2-10-e12129ok.pdfapplication/pdf5880378https://www.arca.fiocruz.br/bitstream/icict/49826/2/JEV2-10-e12129ok.pdf444f1cd866bd88ce44b540a7f86b5a40MD52icict/498262022-01-12 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| dc.title.pt_BR.fl_str_mv |
Cryptococcus extracellular vesicles properties and their use as vaccine platforms |
| title |
Cryptococcus extracellular vesicles properties and their use as vaccine platforms |
| spellingShingle |
Cryptococcus extracellular vesicles properties and their use as vaccine platforms Rizzo, Juliana Cryptococcus Infecções por Fungos Cryptococcosis Extracellular Vesicles Mycoses Fungal Infections Criptococosis Vesículas Extracelulares Micosis Infecciones por Hongos Cryptococcus Vésicules extracellulaires Mycoses Infections fongiques Infecção por Cryptococcus neoformans Vesículas Extracelulares Micoses |
| title_short |
Cryptococcus extracellular vesicles properties and their use as vaccine platforms |
| title_full |
Cryptococcus extracellular vesicles properties and their use as vaccine platforms |
| title_fullStr |
Cryptococcus extracellular vesicles properties and their use as vaccine platforms |
| title_full_unstemmed |
Cryptococcus extracellular vesicles properties and their use as vaccine platforms |
| title_sort |
Cryptococcus extracellular vesicles properties and their use as vaccine platforms |
| author |
Rizzo, Juliana |
| author_facet |
Rizzo, Juliana Wong, Sarah SzeWah Gazi, Anastasia D. Moyrand, Frédérique Chaze, Thibault Commere, Pierre-Henri Novault, Sophie Matondo, Mariette Péhau-Arnaudet, Gérard Reis, Flavia Coelho Garcia dos Vos, Matthijn Alves, Lysangela Ronalte May, Robin C. Nimrichter, Leonardo Rodrigues, Marcio Lourenço Aimanianda, Vishukumar Janbon, Guilhem |
| author_role |
author |
| author2 |
Wong, Sarah SzeWah Gazi, Anastasia D. Moyrand, Frédérique Chaze, Thibault Commere, Pierre-Henri Novault, Sophie Matondo, Mariette Péhau-Arnaudet, Gérard Reis, Flavia Coelho Garcia dos Vos, Matthijn Alves, Lysangela Ronalte May, Robin C. Nimrichter, Leonardo Rodrigues, Marcio Lourenço Aimanianda, Vishukumar Janbon, Guilhem |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Rizzo, Juliana Wong, Sarah SzeWah Gazi, Anastasia D. Moyrand, Frédérique Chaze, Thibault Commere, Pierre-Henri Novault, Sophie Matondo, Mariette Péhau-Arnaudet, Gérard Reis, Flavia Coelho Garcia dos Vos, Matthijn Alves, Lysangela Ronalte May, Robin C. Nimrichter, Leonardo Rodrigues, Marcio Lourenço Aimanianda, Vishukumar Janbon, Guilhem |
| dc.subject.other.pt_BR.fl_str_mv |
Cryptococcus Infecções por Fungos |
| topic |
Cryptococcus Infecções por Fungos Cryptococcosis Extracellular Vesicles Mycoses Fungal Infections Criptococosis Vesículas Extracelulares Micosis Infecciones por Hongos Cryptococcus Vésicules extracellulaires Mycoses Infections fongiques Infecção por Cryptococcus neoformans Vesículas Extracelulares Micoses |
| dc.subject.en.pt_BR.fl_str_mv |
Cryptococcosis Extracellular Vesicles Mycoses Fungal Infections |
| dc.subject.es.pt_BR.fl_str_mv |
Criptococosis Vesículas Extracelulares Micosis Infecciones por Hongos |
| dc.subject.fr.pt_BR.fl_str_mv |
Cryptococcus Vésicules extracellulaires Mycoses Infections fongiques |
| dc.subject.decs.pt_BR.fl_str_mv |
Infecção por Cryptococcus neoformans Vesículas Extracelulares Micoses |
| description |
Institut Pasteur. Département de Mycologie. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. |
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2021 |
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2021-11-12T19:13:45Z |
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2021-11-12T19:13:45Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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RIZZO, Juliana. et al. Cryptococcus extracellular vesicles properties and their use as vaccine platforms. Journal of Extracellular Vesicles, p. 1–19, 2021. |
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https://www.arca.fiocruz.br/handle/icict/49826 |
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2001-3078 |
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10.1002/jev2.12129 |
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RIZZO, Juliana. et al. Cryptococcus extracellular vesicles properties and their use as vaccine platforms. Journal of Extracellular Vesicles, p. 1–19, 2021. 2001-3078 10.1002/jev2.12129 |
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International Society for Extracellular Vesicles |
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International Society for Extracellular Vesicles |
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