A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures

Detalhes bibliográficos
Autor(a) principal: Costa, Eliane Veiga da
Data de Publicação: 2012
Outros Autores: Campos, Renata de Mendonça, Tavares, Fernando Neto, Grégio, Cátia Regina Valério, Burlandy, Fernanda Marcicano, Silva, Edson Elias da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15325
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.
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spelling Costa, Eliane Veiga daCampos, Renata de MendonçaTavares, Fernando NetoGrégio, Cátia Regina ValérioBurlandy, Fernanda MarcicanoSilva, Edson Elias da2016-08-23T11:42:36Z2016-08-23T11:42:36Z2012COSTA, Eliane Veiga da; et al. A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures. Mem Inst Oswaldo Cruz, v.107, n.5, p.698-701, Aug. 2012.1678-8080https://www.arca.fiocruz.br/handle/icict/1532510.1590/S0074-02762012000500022engFundação Oswaldo CruzPoliomieliteVacina AntipólioReação em Cadeia da PolimeraseDiagnostic techniques and proceduresVP1 proteinPoliovirusRT-PCR methodA RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixturesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Outbreaks caused by vaccine-derived polioviruses are challenging the final eradication of paralytic poliomyelitis. Therefore, the surveillance of the acute flaccid paralysis cases based on poliovirus isolation and characterization remains an essential activity. Due to the use of trivalent oral poliovirus vaccine (OPV), mixtures containing more than one serotype of Sabin-related polioviruses are frequently isolated from clinical samples. Because each poliovirus isolate needs to be individually analyzed, we designed polymerase chain reaction primers that can selectively distinguish and amplify a genomic segment of the three Sabin-related poliovirus serotypes present in mixtures, thus, optimizing the diagnosis and providing prompt information to support epidemiologic actions.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15325/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALeliane_costa_etal_IOC_2012.pdfeliane_costa_etal_IOC_2012.pdfapplication/pdf344712https://www.arca.fiocruz.br/bitstream/icict/15325/2/eliane_costa_etal_IOC_2012.pdfcb7628396969b77fb2e7e73d0b7e36aeMD52TEXTeliane_costa_etal_IOC_2012.pdf.txteliane_costa_etal_IOC_2012.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures
title A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures
spellingShingle A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures
Costa, Eliane Veiga da
Poliomielite
Vacina Antipólio
Reação em Cadeia da Polimerase
Diagnostic techniques and procedures
VP1 protein
Poliovirus
RT-PCR method
title_short A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures
title_full A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures
title_fullStr A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures
title_full_unstemmed A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures
title_sort A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures
author Costa, Eliane Veiga da
author_facet Costa, Eliane Veiga da
Campos, Renata de Mendonça
Tavares, Fernando Neto
Grégio, Cátia Regina Valério
Burlandy, Fernanda Marcicano
Silva, Edson Elias da
author_role author
author2 Campos, Renata de Mendonça
Tavares, Fernando Neto
Grégio, Cátia Regina Valério
Burlandy, Fernanda Marcicano
Silva, Edson Elias da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Eliane Veiga da
Campos, Renata de Mendonça
Tavares, Fernando Neto
Grégio, Cátia Regina Valério
Burlandy, Fernanda Marcicano
Silva, Edson Elias da
dc.subject.other.pt_BR.fl_str_mv Poliomielite
Vacina Antipólio
Reação em Cadeia da Polimerase
topic Poliomielite
Vacina Antipólio
Reação em Cadeia da Polimerase
Diagnostic techniques and procedures
VP1 protein
Poliovirus
RT-PCR method
dc.subject.en.pt_BR.fl_str_mv Diagnostic techniques and procedures
VP1 protein
Poliovirus
RT-PCR method
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2016-08-23T11:42:36Z
dc.date.available.fl_str_mv 2016-08-23T11:42:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Eliane Veiga da; et al. A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures. Mem Inst Oswaldo Cruz, v.107, n.5, p.698-701, Aug. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15325
dc.identifier.issn.pt_BR.fl_str_mv 1678-8080
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762012000500022
identifier_str_mv COSTA, Eliane Veiga da; et al. A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures. Mem Inst Oswaldo Cruz, v.107, n.5, p.698-701, Aug. 2012.
1678-8080
10.1590/S0074-02762012000500022
url https://www.arca.fiocruz.br/handle/icict/15325
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/15325/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/15325/2/eliane_costa_etal_IOC_2012.pdf
https://www.arca.fiocruz.br/bitstream/icict/15325/3/eliane_costa_etal_IOC_2012.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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