Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts

Detalhes bibliográficos
Autor(a) principal: Barbosa, Alynne da Silva
Data de Publicação: 2016
Outros Autores: Bastos, Otilio Machado Pereira, Uchôa, Claudia Maria Antunes, Pissinatti, Alcides, Bastos, Augusto César Machado Pereira, Souza, Igo Vieira de, Dib, Laís Verdan, Azevedo, Eduarda Peixoto, Siqueira, Mayara Perlingeiro de, Cardozo, Matheus Lessa, Amendoeira, Maria Regina Reis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17873
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ. Brasil.
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spelling Barbosa, Alynne da SilvaBastos, Otilio Machado PereiraUchôa, Claudia Maria AntunesPissinatti, AlcidesBastos, Augusto César Machado PereiraSouza, Igo Vieira deDib, Laís VerdanAzevedo, Eduarda PeixotoSiqueira, Mayara Perlingeiro deCardozo, Matheus LessaAmendoeira, Maria Regina Reis2017-02-21T14:44:40Z2017-02-21T14:44:40Z2016BARBOSA, Alynne da Silva; et al. Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts. Braz. J. Vet. Parasitol., v. 25, n. 3, p. 286-292, jul.-set. 2016.0103-846Xhttps://www.arca.fiocruz.br/handle/icict/1787310.1590/S1984-296120160441984-2961engFCAV/UNESP - Departamento de Patologia VeterináriaProtozoárioCistosTécnica parasitológicaDiagnóstico laboratorialBalantidium coliBalantidium coliparasitological techniquelaboratory diagnosiscystsComparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cystsComparação de cinco técnicas parasitológicas para diagnóstico laboratorial de cistos de Balantidium coliinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ. Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Instituto Estadual do Ambiente. Centro de Primatologia do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Serviço de Criação de Primatas não humanos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ. Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ. Brasil.Balantidium coli is a protozoon that can cause dysentery in humans, pigs and nonhuman primates, with zoonotic potential. In the literature, there is still little information on the effectiveness of different laboratory techniques for diagnosing this disease. This study compared and evaluated the performance of the Lutz, modified Ritchie, Faust, modified Sheather and direct examination techniques for detecting cysts of this protozoon. Between 2012 and 2014, 1905 fecal samples were collected from captive animals in the state of Rio de Janeiro. Of these, 790 were obtained from the rectum of pigs and 1115 from enclosures occupied by nonhuman primates. B. coli cysts were most evident through direct examination (22.4% of the samples) and the Lutz technique (21%). Fair agreement (Kappa = 0.41; p < 0.05) was observed only between direct examination and Lutz. The flotation techniques (Faust and modified Sheather) did not show good recovery of cysts. A statistically significant difference (p < 0.05) in the frequency of cysts between pigs and nonhuman primates could only be observed through direct examination and the Lutz technique. The most efficient method for diagnosing this parasitosis was seen to an association between direct examination and the spontaneous sedimentation technique.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17873/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALalynne_barbosa_etal_IOC-2016.pdfalynne_barbosa_etal_IOC-2016.pdfapplication/pdf1407725https://www.arca.fiocruz.br/bitstream/icict/17873/2/alynne_barbosa_etal_IOC-2016.pdf65dc501fc626ea83a1e8e27adcbb84d5MD52TEXTalynne_barbosa_etal_IOC-2016.pdf.txtalynne_barbosa_etal_IOC-2016.pdf.txtExtracted texttext/plain34902https://www.arca.fiocruz.br/bitstream/icict/17873/3/alynne_barbosa_etal_IOC-2016.pdf.txt905b3512426551b13492354346716246MD53icict/178732018-08-15 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dc.title.pt_BR.fl_str_mv Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts
dc.title.alternative.pt_BR.fl_str_mv Comparação de cinco técnicas parasitológicas para diagnóstico laboratorial de cistos de Balantidium coli
title Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts
spellingShingle Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts
Barbosa, Alynne da Silva
Protozoário
Cistos
Técnica parasitológica
Diagnóstico laboratorial
Balantidium coli
Balantidium coli
parasitological technique
laboratory diagnosis
cysts
title_short Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts
title_full Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts
title_fullStr Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts
title_full_unstemmed Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts
title_sort Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts
author Barbosa, Alynne da Silva
author_facet Barbosa, Alynne da Silva
Bastos, Otilio Machado Pereira
Uchôa, Claudia Maria Antunes
Pissinatti, Alcides
Bastos, Augusto César Machado Pereira
Souza, Igo Vieira de
Dib, Laís Verdan
Azevedo, Eduarda Peixoto
Siqueira, Mayara Perlingeiro de
Cardozo, Matheus Lessa
Amendoeira, Maria Regina Reis
author_role author
author2 Bastos, Otilio Machado Pereira
Uchôa, Claudia Maria Antunes
Pissinatti, Alcides
Bastos, Augusto César Machado Pereira
Souza, Igo Vieira de
Dib, Laís Verdan
Azevedo, Eduarda Peixoto
Siqueira, Mayara Perlingeiro de
Cardozo, Matheus Lessa
Amendoeira, Maria Regina Reis
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, Alynne da Silva
Bastos, Otilio Machado Pereira
Uchôa, Claudia Maria Antunes
Pissinatti, Alcides
Bastos, Augusto César Machado Pereira
Souza, Igo Vieira de
Dib, Laís Verdan
Azevedo, Eduarda Peixoto
Siqueira, Mayara Perlingeiro de
Cardozo, Matheus Lessa
Amendoeira, Maria Regina Reis
dc.subject.other.pt_BR.fl_str_mv Protozoário
Cistos
Técnica parasitológica
Diagnóstico laboratorial
Balantidium coli
topic Protozoário
Cistos
Técnica parasitológica
Diagnóstico laboratorial
Balantidium coli
Balantidium coli
parasitological technique
laboratory diagnosis
cysts
dc.subject.en.pt_BR.fl_str_mv Balantidium coli
parasitological technique
laboratory diagnosis
cysts
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ. Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-02-21T14:44:40Z
dc.date.available.fl_str_mv 2017-02-21T14:44:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA, Alynne da Silva; et al. Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts. Braz. J. Vet. Parasitol., v. 25, n. 3, p. 286-292, jul.-set. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17873
dc.identifier.issn.pt_BR.fl_str_mv 0103-846X
dc.identifier.doi.none.fl_str_mv 10.1590/S1984-29612016044
dc.identifier.eissn.none.fl_str_mv 1984-2961
identifier_str_mv BARBOSA, Alynne da Silva; et al. Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts. Braz. J. Vet. Parasitol., v. 25, n. 3, p. 286-292, jul.-set. 2016.
0103-846X
10.1590/S1984-29612016044
1984-2961
url https://www.arca.fiocruz.br/handle/icict/17873
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv FCAV/UNESP - Departamento de Patologia Veterinária
publisher.none.fl_str_mv FCAV/UNESP - Departamento de Patologia Veterinária
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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