Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells

Detalhes bibliográficos
Autor(a) principal: Carvalho, J. l.
Data de Publicação: 2020
Outros Autores: Miranda, M., Fialho, A. K., Faria Neto, Hugo Caire C., Anatriello, E., Keller, A. C., Aimbire, Flavio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44782
Resumo: Universidade Federal de São Paulo. Departamento de Ciência e Tecnologia. São José dos Campos, SP, Brasil.
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spelling Carvalho, J. l.Miranda, M.Fialho, A. K.Faria Neto, Hugo Caire C.Anatriello, E.Keller, A. C.Aimbire, Flavio2020-12-10T14:54:02Z2020-12-10T14:54:02Z2020CARVALHO, J. L. et al. Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells. Plos One, v. 15, n. 4, 0225560, 18p, Apr. 2020.1932-6203https://www.arca.fiocruz.br/handle/icict/4478210.1371/journal.pone.0225560engPublic Library of ScienceLactobacillus rhamnosus probióticoAlimentação oralRhamnosus probióticoFumaça do cigarroCamundongos C57Bl / 6:Marcadores inflamatóriosCélulas epiteliais brônquicasHumanosProbiotic Lactobacillus rhamnosusCOPDCigarette smokeC57Bl/6 miceInflammatory markersHuman bronchialEpithelial cellsOral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Departamento de Ciência e Tecnologia. São José dos Campos, SP, Brasil.Universidade Federal de São Paulo. Departamento de Ciência e Tecnologia. São José dos Campos, SP, Brasil.Universidade Federal de São Paulo. Departamento de Ciência e Tecnologia. São José dos Campos, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Paulo. Departamento de Ciência e Tecnologia. São José dos Campos, SP, Brasil.Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Departamento de Ciência e Tecnologia. São José dos Campos, SP, Brasil.COPD is a prevalent lung disease with significant impacts on public health. Affected airways exhibit pulmonary neutrophilia and consequent secretion of pro-inflammatory cytokines and proteases, which result in lung emphysema. Probiotics act as nonspecific modulators of the innate immune system that improve several inflammatory responses. To investigate the effect of Lactobacillus rhamnosus (Lr) on cigarette smoke (CS)-induced COPD C57Bl/6 mice were treated with Lr during the week before COPD induction and three times/week until euthanasia. For in vitro assays, murine bronchial epithelial cells as well as human bronchial epithelial cells exposed to cigarette smoke extract during 24 hours were treated with Lr 1 hour before CSE addition. Lr treatment attenuated the inflammatory response both in the airways and lung parenchyma, reducing inflammatory cells infiltration and the production of pro-inflammatory cytokines and chemokines. Also, Lr-treated mice presented with lower metalloproteases in lung tissue and lung remodeling. In parallel to the reduction in the expression of TLR2, TLR4, TLR9, STAT3, and NF-κB in lung tissue, Lr increased the levels of IL-10 as well as SOCS3 and TIMP1/2, indicating the induction of an anti-inflammatory environment. Similarly, murine bronchial epithelial cells as well as human bronchial epithelial cells (BEAS) exposed to CSE produced pro-inflammatory cytokines and chemokines, which were inhibited by Lr treatment in association with the production of anti-inflammatory molecules. Moreover, the presence of Lr also modulated the expression of COPD-associated transcription found into BALF of COPD mice group, i.e., Lr downregulated expression of NF-κB and STAT3, and inversely upregulated increased expression of SOCS3. Thus, our findings indicate that Lr modulates the balance between pro- and anti-inflammatory cytokines in human bronchial epithelial cells upon CS exposure and it can be a useful tool to improve the lung inflammatory response associated with COPD.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44782/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALHugoCastro_FariaNeto_etal_IOC_2020.pdfHugoCastro_FariaNeto_etal_IOC_2020.pdfapplication/pdf2471174https://www.arca.fiocruz.br/bitstream/icict/44782/2/HugoCastro_FariaNeto_etal_IOC_2020.pdf491046c2e62cd8cbe06337cdcecc0cfeMD52TEXTHugoCastro_FariaNeto_etal_IOC_2020.pdf.txtHugoCastro_FariaNeto_etal_IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells
title Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells
spellingShingle Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells
Carvalho, J. l.
Lactobacillus rhamnosus probiótico
Alimentação oral
Rhamnosus probiótico
Fumaça do cigarro
Camundongos C57Bl / 6:
Marcadores inflamatórios
Células epiteliais brônquicas
Humanos
Probiotic Lactobacillus rhamnosus
COPD
Cigarette smoke
C57Bl/6 mice
Inflammatory markers
Human bronchial
Epithelial cells
title_short Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells
title_full Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells
title_fullStr Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells
title_full_unstemmed Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells
title_sort Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells
author Carvalho, J. l.
author_facet Carvalho, J. l.
Miranda, M.
Fialho, A. K.
Faria Neto, Hugo Caire C.
Anatriello, E.
Keller, A. C.
Aimbire, Flavio
author_role author
author2 Miranda, M.
Fialho, A. K.
Faria Neto, Hugo Caire C.
Anatriello, E.
Keller, A. C.
Aimbire, Flavio
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, J. l.
Miranda, M.
Fialho, A. K.
Faria Neto, Hugo Caire C.
Anatriello, E.
Keller, A. C.
Aimbire, Flavio
dc.subject.other.pt_BR.fl_str_mv Lactobacillus rhamnosus probiótico
Alimentação oral
Rhamnosus probiótico
Fumaça do cigarro
Camundongos C57Bl / 6:
Marcadores inflamatórios
Células epiteliais brônquicas
Humanos
topic Lactobacillus rhamnosus probiótico
Alimentação oral
Rhamnosus probiótico
Fumaça do cigarro
Camundongos C57Bl / 6:
Marcadores inflamatórios
Células epiteliais brônquicas
Humanos
Probiotic Lactobacillus rhamnosus
COPD
Cigarette smoke
C57Bl/6 mice
Inflammatory markers
Human bronchial
Epithelial cells
dc.subject.en.pt_BR.fl_str_mv Probiotic Lactobacillus rhamnosus
COPD
Cigarette smoke
C57Bl/6 mice
Inflammatory markers
Human bronchial
Epithelial cells
description Universidade Federal de São Paulo. Departamento de Ciência e Tecnologia. São José dos Campos, SP, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-12-10T14:54:02Z
dc.date.available.fl_str_mv 2020-12-10T14:54:02Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv CARVALHO, J. L. et al. Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells. Plos One, v. 15, n. 4, 0225560, 18p, Apr. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44782
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0225560
identifier_str_mv CARVALHO, J. L. et al. Oral feeding with probiotic Lactobacillus rhamnosus attenuates cigarette smoke-induced COPD in C57Bl/6 mice: Relevance to inflammatory markers in human bronchial epithelial cells. Plos One, v. 15, n. 4, 0225560, 18p, Apr. 2020.
1932-6203
10.1371/journal.pone.0225560
url https://www.arca.fiocruz.br/handle/icict/44782
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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