Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7340 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil |
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Andrade, Lídia Maria deGeraldo, Jony MarquesGonçalves, Osvaldo XavierLeite, Miguel T. T.Catarina, Anderson M.Guimarães, Melissa MonteiroLeme, Adriana Franco PaesYokoo, SamiMachado, Carlos RobertoRajão, Matheus AndradeCarvalho, Sandhra MariaGomes, Dawidson AssisAguiar, Carla JeaneFagundes, Elaine Maria de SouzaZani, Carlos LeomarResende, Rodrigo RibeiroMartins Filho, Olindo AssisLeite, Maria de Fátima2014-02-19T16:27:35Z2014-02-19T16:27:35Z2012ANDRADE, Lídia Maria et al. Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy. Journal of Cancer Science & Therapy. 2012, vol.04, pp. 131-139.1948-5956https://www.arca.fiocruz.br/handle/icict/7340engOMICS GroupNucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilSão Francisco Radiotherapy Institute. Belo Horizonte, MG, BrazilSão Francisco Radiotherapy Institute. Belo Horizonte, MG, BrazilSão Francisco Radiotherapy Institute. Belo Horizonte, MG, BrazilSão Francisco Radiotherapy Institute. Belo Horizonte, MG, BrazilFederal University of Vales do Jequitinhonha and Mucurí. Diamantina,MG, BrazilNational Laboratory of Biosciences. Campinas, SP, BrazilNational Laboratory of Biosciences. Campinas, SP, BrazilFederal University of Minas Gerais. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. School of Engineering. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Department of Physiology and Biophysics. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Department of Physiology and Biophysics. Belo Horizonte, MG, BrazilRené Rachou Research Center. Belo Horizonte,MG, BrazilFederal University of Minas Gerais. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. René Rachou Research Center. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil/Howard Hughes Medical Institute. USABackground: Calcium (Ca2+) signaling within the nucleus is known to play a crucial role in cell proliferation. The aim of this study was to investigate whether nuclear Ca2+ buffering could improve the antitumor effect of X-rays therapy on Human Squamous Cell Carcinoma (HSCC). Methods: For these purpose, we developed an experimental protocol that simulated clinical radiotherapy and prevented bystander effects of irradiation. HSCC, A431 cell line, was submitted to 10Gy cumulative X-rays therapy alone (XR Cd 10Gy) or in association with the strategy that selectively buffer nuclear Ca 2+ (Ca 2+ n) signaling. Results: Upon Ca 2+ n buffering, A431 cell proliferation rate decreased significantly as compared to control. Cell cycle analysis showed that association of Ca2+ n buffering with XR Cd 10Gy increased the percentage of A431 cells at G 2 /M and did not increase nuclear/mitochondrial DNA damages. Nonetheless, Ca 2+ n buffering prevented the increase of the radioresistance-related biomarker ADAM-17 expression and EGFR activation induced by irradiation. Furthermore, the association therapy almost completely abolished cell survival fraction even using approximately half of the X-rays cumulative dose Conclusions: Nuclear Ca 2+ buffering sensitizes human squamous cell carcinoma to X - rays irradiation treatment.Human squamous cell carcinomaNuclear calcium bufferingX-rays irradiationA431 cellsHead and neck tumorinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALMethylation of ASC, a Pro-apoptotic Gene Associates with NALP1, Inhibits Apoptosis in Human Colon Cancer - JCST-04-131.pdf.pdfMethylation of ASC, a Pro-apoptotic Gene Associates with NALP1, Inhibits Apoptosis in Human Colon Cancer - JCST-04-131.pdf.pdfapplication/pdf2490709https://www.arca.fiocruz.br/bitstream/icict/7340/1/Methylation%20of%20ASC%2c%20a%20Pro-apoptotic%20Gene%20Associates%20with%20NALP1%2c%20Inhibits%20Apoptosis%20in%20Human%20Colon%20Cancer%20-%20JCST-04-131.pdf.pdf37241b1945fb96047c2b5565a08b9671MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7340/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTMethylation of ASC, a Pro-apoptotic Gene Associates with NALP1, Inhibits Apoptosis in Human Colon Cancer - JCST-04-131.pdf.pdf.txtMethylation of ASC, a Pro-apoptotic Gene Associates with NALP1, Inhibits Apoptosis in Human Colon Cancer - JCST-04-131.pdf.pdf.txtExtracted texttext/plain9https://www.arca.fiocruz.br/bitstream/icict/7340/5/Methylation%20of%20ASC%2c%20a%20Pro-apoptotic%20Gene%20Associates%20with%20NALP1%2c%20Inhibits%20Apoptosis%20in%20Human%20Colon%20Cancer%20-%20JCST-04-131.pdf.pdf.txt33f4f15a16a9843faf6a25d4f387b6fdMD55THUMBNAILMethylation of ASC, a Pro-apoptotic Gene Associates with NALP1, Inhibits Apoptosis in Human Colon Cancer - JCST-04-131.pdf.pdf.jpgMethylation of ASC, a Pro-apoptotic Gene Associates with NALP1, Inhibits Apoptosis in Human Colon Cancer - JCST-04-131.pdf.pdf.jpgGenerated Thumbnailimage/jpeg2076https://www.arca.fiocruz.br/bitstream/icict/7340/4/Methylation%20of%20ASC%2c%20a%20Pro-apoptotic%20Gene%20Associates%20with%20NALP1%2c%20Inhibits%20Apoptosis%20in%20Human%20Colon%20Cancer%20-%20JCST-04-131.pdf.pdf.jpg4b6f8ec1b8d908150bf88e640850d934MD54icict/73402018-04-06 08:43:55.992oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:55Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy |
| title |
Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy |
| spellingShingle |
Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy Andrade, Lídia Maria de Human squamous cell carcinoma Nuclear calcium buffering X-rays irradiation A431 cells Head and neck tumor |
| title_short |
Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy |
| title_full |
Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy |
| title_fullStr |
Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy |
| title_full_unstemmed |
Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy |
| title_sort |
Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy |
| author |
Andrade, Lídia Maria de |
| author_facet |
Andrade, Lídia Maria de Geraldo, Jony Marques Gonçalves, Osvaldo Xavier Leite, Miguel T. T. Catarina, Anderson M. Guimarães, Melissa Monteiro Leme, Adriana Franco Paes Yokoo, Sami Machado, Carlos Roberto Rajão, Matheus Andrade Carvalho, Sandhra Maria Gomes, Dawidson Assis Aguiar, Carla Jeane Fagundes, Elaine Maria de Souza Zani, Carlos Leomar Resende, Rodrigo Ribeiro Martins Filho, Olindo Assis Leite, Maria de Fátima |
| author_role |
author |
| author2 |
Geraldo, Jony Marques Gonçalves, Osvaldo Xavier Leite, Miguel T. T. Catarina, Anderson M. Guimarães, Melissa Monteiro Leme, Adriana Franco Paes Yokoo, Sami Machado, Carlos Roberto Rajão, Matheus Andrade Carvalho, Sandhra Maria Gomes, Dawidson Assis Aguiar, Carla Jeane Fagundes, Elaine Maria de Souza Zani, Carlos Leomar Resende, Rodrigo Ribeiro Martins Filho, Olindo Assis Leite, Maria de Fátima |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Andrade, Lídia Maria de Geraldo, Jony Marques Gonçalves, Osvaldo Xavier Leite, Miguel T. T. Catarina, Anderson M. Guimarães, Melissa Monteiro Leme, Adriana Franco Paes Yokoo, Sami Machado, Carlos Roberto Rajão, Matheus Andrade Carvalho, Sandhra Maria Gomes, Dawidson Assis Aguiar, Carla Jeane Fagundes, Elaine Maria de Souza Zani, Carlos Leomar Resende, Rodrigo Ribeiro Martins Filho, Olindo Assis Leite, Maria de Fátima |
| dc.subject.en.pt_BR.fl_str_mv |
Human squamous cell carcinoma Nuclear calcium buffering X-rays irradiation A431 cells Head and neck tumor |
| topic |
Human squamous cell carcinoma Nuclear calcium buffering X-rays irradiation A431 cells Head and neck tumor |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil |
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2012 |
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2012 |
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2014-02-19T16:27:35Z |
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2014-02-19T16:27:35Z |
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info:eu-repo/semantics/article |
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ANDRADE, Lídia Maria et al. Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy. Journal of Cancer Science & Therapy. 2012, vol.04, pp. 131-139. |
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https://www.arca.fiocruz.br/handle/icict/7340 |
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1948-5956 |
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ANDRADE, Lídia Maria et al. Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy. Journal of Cancer Science & Therapy. 2012, vol.04, pp. 131-139. 1948-5956 |
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