Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients

Detalhes bibliográficos
Autor(a) principal: Marin, Anelis Maria
Data de Publicação: 2022
Outros Autores: Mattar, Sibelle Botogosque, Amatuzzi, Rafaela Ferreira, Chammas, Roger, Uno, Miyuki, Zanette, Dalila Luciola, Aoki, Mateus Nóbrega
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54660
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.
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spelling Marin, Anelis MariaMattar, Sibelle BotogosqueAmatuzzi, Rafaela FerreiraChammas, RogerUno, MiyukiZanette, Dalila LuciolaAoki, Mateus Nóbrega2022-08-16T14:36:57Z2022-08-16T14:36:57Z2022MARIN, Anelis Maria et al. Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients. Biomolecules, v. 12, n. 769, p. 1–33, 2022.2218-273Xhttps://www.arca.fiocruz.br/handle/icict/5466010.3390/biom12060769porMDPIPlasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Faculdade de Medicina da Universidade de São Paulo. Departamento de Radiologia e Oncologia. Centro de Investigação Translacional em Oncologia. São Paulo, SP, Brasil. / Faculdade de Medicina da São Paulo. Hospital das Clínicas. São Paulo, SP, Brasil. / Instituto do Câncer do Estado de São Paulo. São Paulo, SP, Brasil.Faculdade de Medicina da Universidade de São Paulo. Departamento de Radiologia e Oncologia. Centro de Investigação Translacional em Oncologia. São Paulo, SP, Brasil. / Faculdade de Medicina da São Paulo. Hospital das Clínicas. São Paulo, SP, Brasil. / Instituto do Câncer do Estado de São Paulo. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Pancreatic cancer represents one of the leading causes of oncological death worldwide. A combination of pancreatic cancer aggressiveness and late diagnosis are key factors leading to a low survival rate and treatment inefficiency, and early diagnosis is pursued as a critical factor for pancreatic cancer. In this context, plasma microRNAs are emerging as promising players due to their non-invasive and practical usage in oncological diagnosis and prognosis. Recent studies have showed some miRNAs associated with pancreatic cancer subtypes, or with stages of the disease. Here we demonstrate plasma exosome-derived microRNA expression in pancreatic cancer patients and healthy individuals from Brazilian patients. Using plasma of 65 pancreatic cancer patients and 78 healthy controls, plasma exosomes were isolated and miRNAs miR-27b, miR-125b-3p, miR-122-5p, miR-21-5p, miR-221-3p, miR-19b, and miR-205-5p were quantified by RT-qPCR. We found that miR125b-3p, miR-122-5p, and miR-205-5p were statistically overexpressed in the plasma exosomes of pancreatic cancer patients compared to healthy controls. Moreover, miR-205-5p was significantly overexpressed in European descendants, in patients with tumor progression and in those who died from the disease, and diagnostic ability by ROC curve was 0.86. Therefore, we demonstrate that these three microRNAs are potential plasma exosome-derived non-invasive biomarkers for the diagnosis and prognosis of Brazilian pancreatic cancer, demonstrating the importance of different populations and epidemiological bias.MicroRNAsExosomePancreatic NeoplasmsBrazilMicroARNsExosomaMicroRNAsExossomoNeoplasias PancreáticasBrasilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/54660/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALbiomolecules-12-00769ok.pdfbiomolecules-12-00769ok.pdfapplication/pdf1070829https://www.arca.fiocruz.br/bitstream/icict/54660/2/biomolecules-12-00769ok.pdf47715ba960f72578a9a15ca1e772637fMD52icict/546602022-08-16 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dc.title.pt_BR.fl_str_mv Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients
title Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients
spellingShingle Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients
Marin, Anelis Maria
MicroRNAs
Exosome
Pancreatic Neoplasms
Brazil
MicroARNs
Exosoma
MicroRNAs
Exossomo
Neoplasias Pancreáticas
Brasil
title_short Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients
title_full Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients
title_fullStr Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients
title_full_unstemmed Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients
title_sort Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients
author Marin, Anelis Maria
author_facet Marin, Anelis Maria
Mattar, Sibelle Botogosque
Amatuzzi, Rafaela Ferreira
Chammas, Roger
Uno, Miyuki
Zanette, Dalila Luciola
Aoki, Mateus Nóbrega
author_role author
author2 Mattar, Sibelle Botogosque
Amatuzzi, Rafaela Ferreira
Chammas, Roger
Uno, Miyuki
Zanette, Dalila Luciola
Aoki, Mateus Nóbrega
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marin, Anelis Maria
Mattar, Sibelle Botogosque
Amatuzzi, Rafaela Ferreira
Chammas, Roger
Uno, Miyuki
Zanette, Dalila Luciola
Aoki, Mateus Nóbrega
dc.subject.en.pt_BR.fl_str_mv MicroRNAs
Exosome
Pancreatic Neoplasms
Brazil
topic MicroRNAs
Exosome
Pancreatic Neoplasms
Brazil
MicroARNs
Exosoma
MicroRNAs
Exossomo
Neoplasias Pancreáticas
Brasil
dc.subject.es.pt_BR.fl_str_mv MicroARNs
Exosoma
dc.subject.decs.pt_BR.fl_str_mv MicroRNAs
Exossomo
Neoplasias Pancreáticas
Brasil
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-08-16T14:36:57Z
dc.date.available.fl_str_mv 2022-08-16T14:36:57Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARIN, Anelis Maria et al. Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients. Biomolecules, v. 12, n. 769, p. 1–33, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54660
dc.identifier.issn.pt_BR.fl_str_mv 2218-273X
dc.identifier.doi.none.fl_str_mv 10.3390/biom12060769
identifier_str_mv MARIN, Anelis Maria et al. Plasma exosome-derived microRNAs as potential diagnostic and prognostic biomarkers in brazilian pancreatic cancer patients. Biomolecules, v. 12, n. 769, p. 1–33, 2022.
2218-273X
10.3390/biom12060769
url https://www.arca.fiocruz.br/handle/icict/54660
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/54660/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/54660/2/biomolecules-12-00769ok.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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