In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Torres, Maria Celeste
Data de Publicação: 2022
Outros Autores: Di Maio, Fatima, Brown, David, Spyer, Moira, Nastouli, Eleni, Brasil, Patrícia, Filippis, Ana Maria Bispo de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/55751
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
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spelling Torres, Maria CelesteDi Maio, FatimaBrown, DavidSpyer, MoiraNastouli, EleniBrasil, PatríciaFilippis, Ana Maria Bispo de2022-11-22T17:52:24Z2022-11-22T17:52:24Z2022TORRES, Maria Celeste et al. In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil. Viruses, v. 14, n. 9, p. 1-16, 2022.1999-4915https://www.arca.fiocruz.br/handle/icict/5575110.3390/v14092006engMDPIIn Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Departament of Neonatology. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.University College London Hospitals NHS Trust. Advanced Pathogen Diagnostics Unit. London, UK.University College London Hospitals NHS Trust. Advanced Pathogen Diagnostics Unit. London, UK / University College London. UCL GOS Institute of Child Health. Department of Infection, Immunity and Inflammation. London, UK.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Chikungunya virus (CHIKV) is an arthropod-borne virus (arbovirus) transmitted by Aedes mosquitoes. The human infection usually manifests as a febrile and incapacitating arthritogenic illness, self-limiting and non-lethal. However, since 2013, CHIKV spreading through the tropics and to the Americas was accompanied by an increasing number of cases of atypical disease presentation, namely severe neuropathies and neonatal infection due to intrapartum vertical transmission. The pathophysiological mechanisms underlying these conditions have not been fully elucidated. However, arbovirus intrahost genetic diversity is thought to be linked to viral pathogenesis. To determine whether particular viral variants could be somehow associated, we analyzed the intrahost genetic diversity of CHIKV in three infected patients with neurological manifestations and three mothers infected during the intrapartum period, as well as their babies following vertical transmission. No statistically supported differences were observed for the genetic variability (nucleotide substitutions/gene length) along the genome between the groups. However, the newborn and cerebrospinal fluid samples (corresponding to virus passed through the placenta and/or the blood-brain barrier (BBB)) presented a different composition of their intrahost mutant ensembles compared to maternal or patient serum samples, even when concurrent. This finding could be consistent with the unidirectional virus transmission through these barriers, and the effect of selective bottlenecks during the transmission event. In addition, a higher proportion of defective variants (insertions/deletions and stop codons) was detected in the CSF and maternal samples and those were mainly distributed within the viral non-structural genes. Since defective viral genomes in RNA viruses are known to contribute to the outcome of acute viral infections and influence disease severity, their role in these atypical cases should be further investigated. Finally, with the in silico approach adopted, we detected no relevant non-conservative mutational pattern that could provide any hint of the pathophysiological mechanisms underlying these atypical cases. The present analysis represents a unique contribution to our understanding of the transmission events in these cases and generates hypotheses regarding underlying mechanisms, that can be explored further.Atypical manifestationsBlood–brain barrierChikungunya virusIntrahost diversityPlacentainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55751/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALIn Depth_Patricia_Brasil_etal_INI_2022.pdfIn Depth_Patricia_Brasil_etal_INI_2022.pdfapplication/pdf1489462https://www.arca.fiocruz.br/bitstream/icict/55751/2/In%20Depth_Patricia_Brasil_etal_INI_2022.pdfc3170bbe40cf803ce8e1c0dc25222b4dMD52icict/557512022-11-22 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dc.title.en_US.fl_str_mv In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil
title In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil
spellingShingle In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil
Torres, Maria Celeste
Atypical manifestations
Blood–brain barrier
Chikungunya virus
Intrahost diversity
Placenta
title_short In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil
title_full In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil
title_fullStr In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil
title_full_unstemmed In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil
title_sort In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil
author Torres, Maria Celeste
author_facet Torres, Maria Celeste
Di Maio, Fatima
Brown, David
Spyer, Moira
Nastouli, Eleni
Brasil, Patrícia
Filippis, Ana Maria Bispo de
author_role author
author2 Di Maio, Fatima
Brown, David
Spyer, Moira
Nastouli, Eleni
Brasil, Patrícia
Filippis, Ana Maria Bispo de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Torres, Maria Celeste
Di Maio, Fatima
Brown, David
Spyer, Moira
Nastouli, Eleni
Brasil, Patrícia
Filippis, Ana Maria Bispo de
dc.subject.en.en_US.fl_str_mv Atypical manifestations
Blood–brain barrier
Chikungunya virus
Intrahost diversity
Placenta
topic Atypical manifestations
Blood–brain barrier
Chikungunya virus
Intrahost diversity
Placenta
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-11-22T17:52:24Z
dc.date.available.fl_str_mv 2022-11-22T17:52:24Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TORRES, Maria Celeste et al. In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil. Viruses, v. 14, n. 9, p. 1-16, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/55751
dc.identifier.issn.en_US.fl_str_mv 1999-4915
dc.identifier.doi.none.fl_str_mv 10.3390/v14092006
identifier_str_mv TORRES, Maria Celeste et al. In Depth Viral Diversity Analysis in Atypical Neurological and Neonatal Chikungunya Infections in Rio de Janeiro, Brazil. Viruses, v. 14, n. 9, p. 1-16, 2022.
1999-4915
10.3390/v14092006
url https://www.arca.fiocruz.br/handle/icict/55751
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/55751/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/55751/2/In%20Depth_Patricia_Brasil_etal_INI_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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