Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46600 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. |
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Torres, Maria CelesteMendonça, Marcos Cesar Lima deRodrigues, Cintia Damasceno dos SantosFonseca, VagnerRibeiro, Mario SergioBrandão, Ana PaulaCunha, Rivaldo Venâncio daDias, Ana IsabelVilas Boas, Lucy SantosFelix, Alvina ClaraPereira, Maira AlvesPinto, Luzia Maria de OliveiraSakuntabhai, AnavajFilppis, Ana Maria Bispo de2021-04-09T14:27:28Z2021-04-09T14:27:28Z2021TORRES, Maria Celeste et al. Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes. Viruses, v. 13, n. 2, 349, Feb. 2021.1999-4915https://www.arca.fiocruz.br/handle/icict/4660010.3390/v13020349engMDPIVírus da DengueSorotipo 2Diversidade intrahostDengue virusSerotype 2Intrahost diversitySevere diseaseDengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), School of Laboratory Medicine and Medical Sciences, Nelson R Mandela School of Medicine, College of Health Sciences, University of KwaZulu-Natal, 4041 Durban, South Africa / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Genética Celular e Molecular. Belo Horizonte, MG, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Coordenação Geral dos Laboratórios de Saúde Pública. Brasília, DF, Brasil.Superintendência Secretaria de Vigilância em Saúde do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Laboratório Central Noel Nutels/LACEN. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Coordenação de Vigilância em Saúde e Laboratórios de Referência. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical. São Paulo, SP, Brasil.undação Ezequiel Dias/LACEN. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Functional Genetics of Infectious Diseases, Department of Global Health, Institut Pasteur. Paris, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.settings Open AccessArticle Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes by Maria Celeste Torres 1,*OrcID,Marcos Cesar Lima de Mendonça 1,Cintia Damasceno dos Santos Rodrigues 1,Vagner Fonseca 2,3,4OrcID,Mario Sergio Ribeiro 5,Ana Paula Brandão 6,Rivaldo Venâncio da Cunha 7OrcID,Ana Isabel Dias 8,Lucy Santos Vilas Boas 8,Alvina Clara Felix 8,Maira Alves Pereira 9,Luzia Maria de Oliveira Pinto 10OrcID,Anavaj Sakuntabhai 11,†,Ana Maria Bispo de Filippis 1,† andon behalf of ZikAction Consortium 1,† 1 Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, 21040-360 Rio de Janeiro, Brazil 2 KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), School of Laboratory Medicine and Medical Sciences, Nelson R Mandela School of Medicine, College of Health Sciences, University of KwaZulu-Natal, 4041 Durban, South Africa 3 Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil 4 Coordenação Geral dos Laboratórios de Saúde Pública/Secretaria de Vigilância em Saúde, Ministério da Saúde, (CGLAB/SVS-MS) Brasília, 70719-040 Distrito Federal, Brazil 5 Superintendência Secretaria de Vigilância em Saúde do Estado do Rio de Janeiro, 20031-142 Rio de Janeiro, Brazil 6 Laboratório Central Noel Nutels/LACEN, 20231-092 Rio de Janeiro, Brazil 7 Coordenação de Vigilância em Saúde e Laboratórios de Referência da Fundação Oswaldo Cruz, FIOCRUZ, 21040-360 Rio de Janeiro, Brazil 8 Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, 05403-000 São Paulo, Brazil 9 Fundação Ezequiel Dias/LACEN, 31630-903 Belo Horizonte, Brazil 10 Laboratório de Imunologia Viral, Instituto Oswaldo Cruz, Fiocruz, 21040-360 Rio de Janeiro, Brazil 11 Functional Genetics of Infectious Diseases, Department of Global Health, Institut Pasteur, 75015 Paris, France * Author to whom correspondence should be addressed. † These authors contributed equally to this work. Academic Editor: Didier Musso Viruses 2021, 13(2), 349; https://doi.org/10.3390/v13020349 Received: 27 December 2020 / Revised: 7 February 2021 / Accepted: 13 February 2021 / Published: 23 February 2021 (This article belongs to the Special Issue Endemic Arboviruses) Download PDF Browse Figures Citation Export Abstract Intrahost genetic diversity is thought to facilitate arbovirus adaptation to changing environments and hosts, and it might also be linked to viral pathogenesis. Dengue virus serotype 2 (DENV-2) has circulated in Brazil since 1990 and is associated with severe disease and explosive outbreaks. Intending to shed light on the viral determinants for severe dengue pathogenesis, we sought to analyze the DENV-2 intrahost genetic diversity in 68 patient cases clinically classified as dengue fever (n = 31), dengue with warning signs (n = 19), and severe dengue (n = 18). Unlike previous DENV intrahost diversity studies whose approaches employed PCR, here we performed viral whole-genome deep sequencing from clinical samples with an amplicon-free approach, representing the real intrahost diversity scenario. Striking differences were detected in the viral population structure between the three clinical categories, which appear to be driven mainly by different infection times and selection pressures, rather than being linked with the clinical outcome itself. Diversity in the NS2B gene, however, showed to be constrained, irrespective of clinical outcome and infection time. Finally, 385 non-synonymous intrahost single-nucleotide variants located along the viral polyprotein, plus variants located in the untranslated regions, were consistently identified among the samples. Of them, 124 were exclusively or highly detected among cases with warning signs and among severe cases. However, there was no variant that by itself appeared to characterize the cases of greater severity, either due to its low intrahost frequency or the conservative effect on amino acid substitution. Although further studies are necessary to determine their real effect on viral proteins, this heightens the possibility of epistatic interactions. The present analysis represents an initial effort to correlate DENV-2 genetic diversity to its pathogenic potential and thus contribute to understanding the virus’s dynamics within its human host.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALMariaCelesteTorres_AnaMFilippis_etal_IOC_2021.pdfMariaCelesteTorres_AnaMFilippis_etal_IOC_2021.pdfapplication/pdf5395613https://www.arca.fiocruz.br/bitstream/icict/46600/2/MariaCelesteTorres_AnaMFilippis_etal_IOC_2021.pdf53651fa5cc6513971ef6b597923e22deMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46600/1/license.txt5a560609d32a3863062d77ff32785d58MD51TEXTMariaCelesteTorres_AnaMFilippis_etal_IOC_2021.pdf.txtMariaCelesteTorres_AnaMFilippis_etal_IOC_2021.pdf.txtExtracted 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Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes |
| title |
Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes |
| spellingShingle |
Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes Torres, Maria Celeste Vírus da Dengue Sorotipo 2 Diversidade intrahost Dengue virus Serotype 2 Intrahost diversity Severe disease |
| title_short |
Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes |
| title_full |
Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes |
| title_fullStr |
Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes |
| title_full_unstemmed |
Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes |
| title_sort |
Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes |
| author |
Torres, Maria Celeste |
| author_facet |
Torres, Maria Celeste Mendonça, Marcos Cesar Lima de Rodrigues, Cintia Damasceno dos Santos Fonseca, Vagner Ribeiro, Mario Sergio Brandão, Ana Paula Cunha, Rivaldo Venâncio da Dias, Ana Isabel Vilas Boas, Lucy Santos Felix, Alvina Clara Pereira, Maira Alves Pinto, Luzia Maria de Oliveira Sakuntabhai, Anavaj Filppis, Ana Maria Bispo de |
| author_role |
author |
| author2 |
Mendonça, Marcos Cesar Lima de Rodrigues, Cintia Damasceno dos Santos Fonseca, Vagner Ribeiro, Mario Sergio Brandão, Ana Paula Cunha, Rivaldo Venâncio da Dias, Ana Isabel Vilas Boas, Lucy Santos Felix, Alvina Clara Pereira, Maira Alves Pinto, Luzia Maria de Oliveira Sakuntabhai, Anavaj Filppis, Ana Maria Bispo de |
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author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Torres, Maria Celeste Mendonça, Marcos Cesar Lima de Rodrigues, Cintia Damasceno dos Santos Fonseca, Vagner Ribeiro, Mario Sergio Brandão, Ana Paula Cunha, Rivaldo Venâncio da Dias, Ana Isabel Vilas Boas, Lucy Santos Felix, Alvina Clara Pereira, Maira Alves Pinto, Luzia Maria de Oliveira Sakuntabhai, Anavaj Filppis, Ana Maria Bispo de |
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Vírus da Dengue Sorotipo 2 Diversidade intrahost |
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Vírus da Dengue Sorotipo 2 Diversidade intrahost Dengue virus Serotype 2 Intrahost diversity Severe disease |
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Dengue virus Serotype 2 Intrahost diversity Severe disease |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. |
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2021-04-09T14:27:28Z |
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TORRES, Maria Celeste et al. Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes. Viruses, v. 13, n. 2, 349, Feb. 2021. |
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https://www.arca.fiocruz.br/handle/icict/46600 |
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1999-4915 |
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10.3390/v13020349 |
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TORRES, Maria Celeste et al. Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes. Viruses, v. 13, n. 2, 349, Feb. 2021. 1999-4915 10.3390/v13020349 |
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